Insecticidal Effects of the Annonaceous Acetogenin Squamocin and the Acetogenin Fraction of Seeds of Rollinia occidentalis on Soybean and Corn Pests

Diego Tolosa¹, José Ruiz Hidalgo¹, Pedro Eugenio Sal¹, Susana Popich², Alicia Bardón^1,3, Adriana Neske^1*

¹Instituto de Química Orgánica, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Ayacucho, Argentina.
²Universidad Nacional de Chilecito, Chilecito, Argentina.
³INQUINOA-CONICET, Tucumán, Argentina.

DOI: 10.4236/jacen.2014.34019                

Abstract

A treatment based on the acetogenin fraction of the methanol extract of Rollinia occidentalis seeds was applied to soybean crops for three consecutive years. In relation to the control population, the treatment reduced the population of Anticarsia gemmatalis, Rachiplusia nu, Pseudoplusia includens, Loxostege bifidalis and Spodoptera frugiperda to 52% and 65% after 48 h of application at concentrations of 500 and 750 μg/mL respectively, while low toxic effects were detected on natural enemies. The extract treatment at 500 μg/mL and a solution of the annonaceous acetogenin, squamocin, at 50 and 100 μg/mL, were also applied to a corn field to produce 75%, 93% and 100% mortality rates on the population of S. frugiperda, respectively, after 72 h of application. In addition, damages caused by lepidopterans in treated crops were lower than those observed in non treated fields, evaluated by residual biomass. This statement is based on data from trials with the commercially available insecticides lufenuron and cypermethrin.

Keywords

Annonaceous Acetogenins, Polyphagous Lepidopteran, Soybean Crops, Corn Crops, Spodoptera frugiperda

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Alkofahi, A., Rupprecht, J.K., Anderson, J.E., McLaughlin, J.L., Mikolajczak, K. and Scout, B. (1989) Search for New Pesticides from Higher Plants. In: Arnason, J.T., Philogene, B. and Morand, A., Eds., Insecticides of Plant Origin, ACS, Washington DC, 25-43. http://dx.doi.org/10.1021/bk-1989-0387.ch003
[2]	Alali, F., Liu, X. and Mc Laughlin, J.L. (1999) Annonaceous Acetogenins: Recent Progress. Journal of Natural Products, 62, 504-540. http://dx.doi.org/10.1021/np980406d
[3]	Guadaño, A., Gutiérrez, C., de La Peña, E., Cortes, D. and González-Coloma, A. (2000) Insecticidal and Mutagenic Evaluation of Two Annonaceous Acetogenins. Journal of Natural Products, 63, 773-776. http://dx.doi.org/10.1021/np990328+
[4]	Hincapié Llanos, C.A., Lopera Arango, D. and Ceballos Giraldo, M. (2008) Insecticidal Activity of Annona muricata (Annonaceae) Seed Extracts on Sitophilus zeamais (Coleoptera: Curculionidae). Revista Colombiana de Entomología, 34, 76-82.
[5]	álvarez Colom, O., Neske, A., Popich, S. and Bardón, A. (2007) Toxic Effects of Annonaceous Acetogenins from Annona cherimolia (Magnoliales: Annonaceae) on Spodoptera frugiperda (Lepidoptera: Noctuidae). Journal of Pest Science, 80, 63-67. http://dx.doi.org/10.1007/s10340-006-0149-2
[6]	álvarez Colom, O., Barrachina, I., Ayala Mingol, I., González Mas, M.C., Moya Sanz, P., Neske, A. and Bardón, A. (2008) Toxic Effects of Annonaceous Acetogenins on Oncopeltus fasciatus. Journal of Pest Science, 81, 85-89. http://dx.doi.org/10.1007/s10340-007-0189-2
[7]	álvarez Colom, O., Salvatore, A., Willink, E., Ordóñez, R., Isla, M.I., Neske, A. and Bardón, A. (2010) Insecticidal, Mutagenic and Genotoxic Evaluation of Annonaceous Acetogenins. Natural Product Communications, 5, 391-394.
[8]	Di Toto Blessing, L., álvarez Colom, O., Popich, S., Neske, A. and Bardón, A. (2010) Antifeedant and Toxic Effects of Acetogenins from Annona montana on Spodoptera frugiperda. Journal of Pest Science, 83, 307-310. http://dx.doi.org/10.1007/s10340-010-0299-0
[9]	Marenco, R.J., Foster, R.E. and Sanchez, C.A. (1992) Sweet Corn Response to Fall Armyworm (Lepidoptera: Noctuidae) Damage during Vegetative Growth. Journal of Economic Entomology, 85, 1285-1292.
[10]	Kumar, H. and Mihn, J.A. (2002) Fall Armyworm (Lepidoptera: Noctuidae), Southwestern Corn Borer (Lepidoptera: Pyralidae) and Sugarcane Borer (Lepidoptera: Pyralidae) Damage and Grain Yield of Four Maize Hybrids in Relation to Four Tillage Systems. Crop Protection, 21, 121-128. http://dx.doi.org/10.1016/S0261-2194(01)00071-0
[11]	Clavijo, S. and Pérez Greiner, G. (2000) Protección y sanidad vegetal (capítulo 6), Insectos plagas del maíz (sección 2). In: Fontana Nieves, H. and González Narváez, C., Eds., Fundación Polar, Caracas, 345-361.
[12]	Murillo, A. (1991) Distribución, importancia y manejo del complejo Spodoptera en Colombia. Memorias Seminario Spodoptera frugiperda (El gusano cogollero) en sorgo, maíz y otros cultivos. In: Zuluaga, J.L. and Muñoz, G., Eds., Calí, Colombia, 15-23.
[13]	Sparks, A.N. (1979) A Review of the Biology of the Fall Armyworm. Florida Entomologist, 62, 82-87. http://dx.doi.org/10.2307/3494083
[14]	Murúa, M.G., Virla, E.G. and Defagó, V. (2003) Evaluación de cuatro dietas artificiales para la cría de Spodoptera frugiperda (Lep.: Noctuidae) destinada a mantener poblaciones experimentales de himenópteros parasitoides. Boletín de Sanidad Vegetal de Plagas, 29, 43-51.
[15]	Murúa, M.G. and Virla, E. (2004) Population Parameters of Spodoptera frugiperda (Smith) (Lep.: Noctuidae) Fed on Corn and Two Predominant Grasess in Tucumán (Argentina). Acta Zoológica Mexicana, 20, 199-210.
[16]	Tolosa, D., álvarez Colom, O., Bardón, A. and Neske, A. (2012) Insecticidal Effects of Annonaceous Acetogenins from Rollinia occidentalis Seeds Extract on Spodoptera frugiperda. Natural Product Communications, 7, 1645-1646.
[17]	Kawasu, K., Alcántara, J. and Kobayashi, A. (1989) Isolation and Structure of Neoannonin, a Novel Insecticidal Compound from the Seeds of Annona squamosa. Agricultural and Biological Chemistry, 53, 2719-2722. http://dx.doi.org/10.1271/bbb1961.53.2719