Modulation Possibilities of the Tissue Wettability, Marker of the Physical Modifications Produced by Senescence versus Photo Senescence at Tegument Level


We used an experimental model involving white Westar rats (young and old) who were subjected to precise doses of ultraviolet radiation (UV). The experiment aims to examine the influence of epidermis potential protection factors in photo senescence versus senescence. We have studied the in vivo effect of some natural polyphones extracted from black grapes seeds and zinc aspartate and in vitro effect of AED (deuterium depleted water). The substances were administered by intragastric gavages, at two days intervals for two weeks prior to UV irradiation and during the entire period of the experiment (28 days). In the end, we determined the degree of hydrophilicity of the skin, in vitro, by measuring the contact angle value, which was inversely proportional to the hydrophilicity of the tissue (the angle formed at the contact between a liquid and a solid surface). Using this method we found a large variability depending on the hydrophilicity of the epidermis (abdomen and back), the age of the animal and UV/non UV irradiation. The maintenance of the epidermis fragments in AED (for 6 hours) increased significantly the tissue wet ability degree both in the young rats subject to UV and in the senescent ones subject to UV (photo senescence).

Share and Cite:

Badescu, L. , Cotrutz, C. , Lupusoru, R. , Diaconescu, B. , Ciocoiu, M. and Badescu, M. (2014) Modulation Possibilities of the Tissue Wettability, Marker of the Physical Modifications Produced by Senescence versus Photo Senescence at Tegument Level. Journal of Biomedical Science and Engineering, 7, 1049-1055. doi: 10.4236/jbise.2014.713102.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Cang, S., Fisher, G.J. and Voorhees, J.J. (2007) Photoaging; Therapy, Pathogenesis, and Prevention. Archives of Dermatology, 133, 1280-1284.
[2] Bissett, D.L., Majeti, S., Fu, J.J., McBride, J. and Wyder, W. (1998) Protective Effect of Topically Applied Conjugated Hexadienes against Ultraviolet Radiation-Induced Chronic Skin Damage in the Hairless Mouse. Photodermatology, Photoimmunology & Photomedicine, 7, 63-67.
[3] Chahary, A., Marcoux, Y., Karimi-Busheri, F. and Tredget, E.E. (2001) Keratinocyte Differentiation Inversely Regulates the Expression of Invo-lucrin and Transforming Growth Factor Beta 1. Journal of Cellular Biochemistry, 83, 239-248.
[4] Chatterjee, R., Benzinger, M.J., Ritter, J.L. and Bissett, D.L. (2007) Chronic Ultraviolet B Radiation-Induced Biochemical Changes in the Skin of Hairless Mice. Photochemistry and Photobiology, 51, 91-97.
[5] Deters, A., Schnetz, E., Schmist, M. and Hensel, A. (2003) Effects of Zinc Histidine and Zinc Sulfate on Natural Human Keratinocytes. Forschende Komplementarmedizin und klassische Naturheilkunde, 10, 19-25.
[6] Fuyimoto, W., Nakamishi, G., Arata, G. and Jetten, A.M. (1997) Differential Expression of Human Cornifin and in Squamous Differential Epithelial Tissues and Several Skin Diseases. Journal of Investigative Dermatology, 108, 200-204.
[7] Landman, L. (2006) The Epidermal Permeability Barrier: Transformation of Lamelar Desks into Intercelular Sheets by a Membrane Fussion Process, a Freeze Fracture Study. Journal of Investigative Dermatology, 887, 202-209.
[8] Fisher, G.J., Wang, Z.Q., Datta, S.C. and Voorhees, J.J. (1998) Pathophysiology of Skin Aging Induced by Ultraviolet Light. New England Journal of Medicine, 237, 412-420.
[9] Brossaud, F. (2009) Composition en Flavonoids des Baieset des Vins de Vitis Vinifera var. Cabernet Franc.These de Doctorat, Angers, 30-35.
[10] Hacquist, G., Von Hevesy, G., Kiss, I., Chen, S. and Tramposch, K.M. (1958) Deuterium and Radiation Sensitivity. Acta Radiologica, 49, 321.
[11] Neldner, K.H. (1990) The Biochemistry and Physiology of Zinc Metabolism in Physiology, Biochemistry and Molecular. Skin Biology, 50, 1329-1350.
[12] Maret, W. (2002) The Function of Zinc Metallothionein: A Link between Cellular Zinc and Redox State. Journal of Nutrition, 130, 1455S-1458S.
[13] Sasaki, H., Akamatsu, H. and Horio, T. (2000) Protective Role of Copper, Zinc Superoxid Dismutase Against UVB- Induced Injury of Human Keratinocyte. Journal of Investigative Dermatology, 114, 502-507.
[14] Somlyai, G., Jancsó, G., Jákli, G., Berkényi, T., Gyongyi, Z. and Ember, I. (2001) The Biological Effect of Deuterium Depleted Water, a Possible New Tool in Cancer Therapy. Anticancer Research, 21, 16-17.
[15] Zheng, P. and Kligman, L.H. (1993) UVA-Induced Ultrastructural Changes in Hairless Mouse Skin: A Comparison to UVB-Induced Damage. Journal of Investigative Dermatology, 100, 194-199.

Copyright © 2023 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.