Colon Adenocarcinoma Diagnosis in Human Samples by Multicontrast Nonlinear Optical Microscopy of Hematoxylin and Eosin Stained Histological Sections
Javier Adur1,2,3, Mariana Bianchi1, Vitor B. Pelegati3, Silvia Viale1, María F. Izaguirre1, Hernandes F. Carvalho3,4, Carlos L. Cesar4,5, Víctor H. Casco1*
1Microscopy Laboratory Applied to Molecular and Cellular Studies, School of Engineering, National University of Entre Ríos (UNER), Oro Verde, Argentina.
2Research and Transfer Center of Entre Ríos (CITER) CONICET-UNER, Oro Verde, Argentina.
3INFABiC-National Institute of Science and Technology on Photonics Applied to Cell Biology, Campinas, Brazil.
4Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, Brazil.
5Biophotonic Group, Optics and Photonics Research Center (CEPOF), Institute of Physics “Gleb Wataghin”, State University of Campinas (UNICAMP), Campinas, Brazil.
DOI: 10.4236/jct.2014.513127   PDF   HTML   XML   4,408 Downloads   5,101 Views   Citations


Combined multimodal nonlinear optical (NLO) microscopies were used to detect and quantify morphological changes associated with stroma and epithelial transformation in colon cancer. Our findings provide complementary information about tissue microstructure, displaying distinctive patterns between normal and malignant human colon. Additionally, we have demonstrated the usefulness of using fixed tissues for the disease diagnostic and prognostic. The present work provides a framework for using NLO techniques as a clinical diagnostic tool for human colon cancer. NLO metrics could be applied to other disorders, which are characterized by abnormal cell proliferation and collagen assembly.

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Adur, J. , Bianchi, M. , Pelegati, V. , Viale, S. , Izaguirre, M. , Carvalho, H. , Cesar, C. and Casco, V. (2014) Colon Adenocarcinoma Diagnosis in Human Samples by Multicontrast Nonlinear Optical Microscopy of Hematoxylin and Eosin Stained Histological Sections. Journal of Cancer Therapy, 5, 1259-1269. doi: 10.4236/jct.2014.513127.

Conflicts of Interest

The authors declare no conflicts of interest.


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