Colon Adenocarcinoma Diagnosis in  Human Samples by Multicontrast  Nonlinear Optical Microscopy of  Hematoxylin and Eosin Stained  Histological Sections

Javier Adur; Mariana Bianchi; Vitor B. Pelegati; Silvia Viale; María F. Izaguirre; Hernandes F. Carvalho; Carlos L. Cesar; Víctor H. Casco

doi:10.4236/jct.2014.513127

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JCT> Vol.5 No.13, November 2014

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Colon Adenocarcinoma Diagnosis in Human Samples by Multicontrast Nonlinear Optical Microscopy of Hematoxylin and Eosin Stained Histological Sections

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DOI: 10.4236/jct.2014.513127 4,088 Downloads 4,535 Views Citations

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Javier Adur^1,2,3, Mariana Bianchi¹, Vitor B. Pelegati³, Silvia Viale¹, María F. Izaguirre¹, Hernandes F. Carvalho^3,4, Carlos L. Cesar^4,5, Víctor H. Casco^1*

Affiliation(s)

¹Microscopy Laboratory Applied to Molecular and Cellular Studies, School of Engineering, National University of Entre Ríos (UNER), Oro Verde, Argentina.
²Research and Transfer Center of Entre Ríos (CITER) CONICET-UNER, Oro Verde, Argentina.
³INFABiC-National Institute of Science and Technology on Photonics Applied to Cell Biology, Campinas, Brazil.
⁴Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, Brazil.
⁵Biophotonic Group, Optics and Photonics Research Center (CEPOF), Institute of Physics “Gleb Wataghin”, State University of Campinas (UNICAMP), Campinas, Brazil.

ABSTRACT

Combined multimodal nonlinear optical (NLO) microscopies were used to detect and quantify morphological changes associated with stroma and epithelial transformation in colon cancer. Our findings provide complementary information about tissue microstructure, displaying distinctive patterns between normal and malignant human colon. Additionally, we have demonstrated the usefulness of using fixed tissues for the disease diagnostic and prognostic. The present work provides a framework for using NLO techniques as a clinical diagnostic tool for human colon cancer. NLO metrics could be applied to other disorders, which are characterized by abnormal cell proliferation and collagen assembly.

KEYWORDS

Nonlinear Microscopy, Nonlinear Multicontrast, Histological Sections, Human Colon Cancer

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Adur, J. , Bianchi, M. , Pelegati, V. , Viale, S. , Izaguirre, M. , Carvalho, H. , Cesar, C. and Casco, V. (2014) Colon Adenocarcinoma Diagnosis in Human Samples by Multicontrast Nonlinear Optical Microscopy of Hematoxylin and Eosin Stained Histological Sections. Journal of Cancer Therapy, 5, 1259-1269. doi: 10.4236/jct.2014.513127.

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