Protein Expression of STAT3, pSTAT3, MMP-7 and VEGF in Colorectal Adenocarcinoma: An Immunohistochemical Study
Rakesh Naidu1*, Lim Vin Nee2, Mak Joon Wah2, Kevin Moissinac3, A. Rahman A. Jamal4, Isa Mohd Rose5, Yunus Gul Alif Gul6, Patricia Lim Kim Chooi2, Gregory Tan Jin San7
1School of Medicine and Health Sciences, Monash University Malaysia, Selangor, Malaysia.
2International Medical University, Kuala Lumpur, Malaysia.
3Department of Surgery, Penang Medical College, Pulau Pinang, Malaysia.
4Department of Paediatrics, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
5Department of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
6Department of Surgery, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Selangor, Malaysia.
7School of Health Sciences, The University of Notre Dame Australia, Fremantle, Australia.
DOI: 10.4236/jct.2014.513119   PDF    HTML   XML   3,030 Downloads   3,779 Views   Citations


Background: The purpose of the present study is to investigate the expression levels of STAT3, pSTAT3, MMP-7 and VEGF in colorectal adenocarcinoma, and also to determine association with the clinico-pathological parameters and co-expression of these genes. Methods: An immunohistochemical method was used to evaluate the expression of MMP-7 and VEGF genes in 93 archival tissues whereas STAT3 and pSTAT3 expression was determined in 75 cases. Results: Overexpression of STAT3 was detected in 26.7% (20/75), pSTAT3 in 13.4% (10/75), MMP-7 in 38.8% (36/93) and VEGF in 59.2% (55/93) of the colorectal carcinomas. STAT3, MMP-7 and VEGF immunopositivity were significantly correlated with poorly-differentiated tumors (P = 0.004; P = 0.03; P = 0.002, respectively) but not with other parameters. However, pSTAT3 immunostaining was not significantly associated with the clinico-pathological characteristics. Significant relationship was noted between overexpression of pSTAT3 and STAT3 (P < 0.001), pSTAT3 and VEGF (P = 0.044), pSTAT3 and MMP-7 (P = 0.003), and STAT3 and VEGF (P = 0.037) but marginal association was detected between STAT3 and MMP-7 (P = 0.057), and MMP-7 and VEGF (P = 0.052). Conclusion: Our data suggest that expression of these genes may have an important role in tumor dedifferentiation and may be useful as indicators of biologic aggressiveness. Co-expression of the biomarkers by cancer cells may have important implications in colorectal cancer biology and could be useful biological markers of the malignant phenotype.

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Naidu, R. , Nee, L. , Wah, M. , Moissinac, K. , Jamal, A. , Rose, I. , Gul, Y. , Chooi, P. and San, G. (2014) Protein Expression of STAT3, pSTAT3, MMP-7 and VEGF in Colorectal Adenocarcinoma: An Immunohistochemical Study. Journal of Cancer Therapy, 5, 1175-1185. doi: 10.4236/jct.2014.513119.

Conflicts of Interest

The authors declare no conflicts of interest.


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