Response Proliferative Capacity of Undifferentiated Stem Cells of Obtained Human Adult Dental Follicle

Larissa Kim Higashi de Carvalho^1,2, Aline Vieira Pinheiro de Araujo^1,2, Manuela Garcia Laveli da Silva^1,2, Rosa Andréa Nogueira Laiso^1,2, Durvanei Augusto Maria^2*

¹Faculty of Medicine, University of Sao Paulo, Sao Paulo, Brazil.
²Laboratory of Biochemistry and Biophysics, Butantan Institute, Sao Paulo, Brazil.

DOI: 10.4236/scd.2014.44013                

Abstract

Objective: The aim of this study was correlation proliferative activity, markers express stem cells, and lipid peroxides of undifferentiated stem cells of human adult dental follicle (DF) following culture. Methods: For this study, we used 8 samples from DF of impacted third molars to maintain culture conditions and evaluated the growth curve, cell viability, production of lipid peroxidation, cell cycle phases, and proliferative index during 25 days of culture. Results: Cells after culture showed characteristics of fibroblast-like type following 25th day of culture. The results of lipid peroxidation showed that stem cells in culture produce 13 nmoles/ml malondialdehyde at the start of culture, increasing until the 12th day and then began a decline that lasted until the 25th day. We revealed that DFSCs presented a significantly higher percentage of cells in S + G2/M phases by the 15th day of culture compared with cells at the start of culture. Cell surface markers revealed that cell lines were negative for HLA-DR and positive for CD90, CD44, and CD105. The expression of p21 protein, involved in the regulation of the cell cycle, showed a significant increase from the 15th to 25th day of culture. Results of cell division rates show a significant increase between the 6th and 15th day of culture. Conclusions: We conclude that the culture remained stable during the 25 days of culture, presenting the markers of stem cells and markers of control, progression, and cell proliferation that there was an increased production of lipid peroxides between the 6th and 12th days; this increase is related to the increased numbers of cells that also occurs during this period. Then, there is a significantly decline in the production of lipid peroxides and the number of cells, which is accompanied by an increase in cell unviability.

Keywords

Follicle Dental Stem Cells, Lipid Peroxidation, Cell Cycle, Proliferation

Conflicts of Interest

The authors declare no conflicts of interest.

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