Malpighian Tubules in Larvae of Diatraea saccharalis (Lepidoptera; Crambidae): A Morphological Comparison between Non-Parasitized and Parasitized by Cotesia flavipes (Hymenoptera; Braconidae)

Gislei Maria Rigoni¹, Helio Conte²

¹Departamento de Ciências Biológicas, Universidade Estadual do Centro Oeste (UNICENTRO), Guarapuava, Brazil.
²Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá (UEM), Maringá, Brazil.

DOI: 10.4236/ae.2014.24029                

Abstract

In Diatraea saccharalis larvae, the Malpighian tubules are found along the digestive tube, extending from the middle mesenteric region to the end of the posterior intestine, where they come in contact with the rectum to form the cryptonephridium. Scanning and transmission electron microscopy of non-parasitized and parasitized larvae by Cotesia flavipes have indicated that the tubules consist of secretory and reabsorption cells. In parasitized larvae, the occurrence of hemocytes and teratocytes around the tubules is indicative of their role in immunological defense; however, they were not observed in non-parasitized larvae. At day 9 of parasitism, the mitochondria-containing vacuoles and myelin-like figures show signs of degeneration. The results of this study have confirmed that C. flavipes manipulates the physiology and biochemistry of D. saccharalis because the Malpighian tubules of the parasitized larvae remain active until the parasitoid completes its pupal stage and is released from the host organism.

Keywords

Endoparasitism, Sugarcane Borer, Morphology, Cotesia flavipes, Biological Control

Conflicts of Interest

The authors declare no conflicts of interest.

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