Research of Submerge Fermentation Conditions on Agaricus blazei Murill

Xinzhi Cao, Yingqing Zhao, Li Xiong, Linsheng Ren, Jia Liu

Department of Bioengineering, Sichuan University of Science and Engineering, Zigong, China.

DOI: 10.4236/fns.2014.519204                

Abstract

Rotational speed, temperature, inoculation amount and pH are the four factors that affect the submerge fermentation of Agaricus blazei Murill. The orthogonal experiment was on the basis of single factor experiments. And the optimal submerge fermentation condition of Agaricus blazei Murill was determined through orthogonal test. The results were as follows: temperature (25℃), rotational speed (150 r/min), inoculation amount (6%) and pH (7), in this condition the cell dry weight reached 1.32 g/100mL; temperature (25℃), rotational speed (150 r/min), inoculation amount (8%) and pH (6.5) in this condition the extracellular polysaccharide reached 6.95 mg/mL.

Keywords

Agaricus blazei Murill, Submerged Fermentation, Cell Dry Weight, Extracellular Polysaccharide

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Wang, Y., Nie, Z.Y., He, Y., et al. (2009) Study on the Extraction of Agaricus blazei Murill Polysaccharide with Compound Enzymes under Ultrasonic Wave. Natural Product Research and Development, 21, 866-870.
[2]	Lin, R.B., Lin, C.Q., Zhang, H., et al. (2012) Advances in Research on the Nutritional and Medicinal Value of Agaricus blazei Murill. Acta Edulis Fungi, 19, 117-122.
[3]	Jia, W., Yan, S.L. and Fan, H. (2010) Effect of a Polysaccharide Fraction from Agaricus blazei Fruit Bodies on Murine Immunological Function. Acta Edulis Fungi, 17, 65-68.
[4]	Ge, H.X. and Liao, W. (2012) Progress of the Polysaccharides of Agaricus blazei Murill. Chinese Journal of Animal Husbandry and Veterinary Medicine, 4-5.
[5]	Ding, C.Y., Zhang, X.X., Zhang, C., et al. (2008) Alkaline Extraction Process of Intracellular Polysaccharides from Agaricus blazei Murill. Chinese Journal of Bioprocess Engineering, 6, 21-26.
[6]	Liu, Y., Chen, Y.R. and Chen, Z.F. (2004) Study on Effect Factors of Boletus sp. Liquid Fermentation for Polysaccharides Preperation. Chinese Journal of Bioprocess Engineering, 2, 44-47.
[7]	Zhou, S., Tang, Q.J., Yang, Y., et al. (2010) Comparison of the Efficacy of Six Different Procedures for Determining Protein in Crude Polysaccharide Preparations from Medicinal Fungi. Acta Edulis Fungi, 17, 72-75.
[8]	Liu, D.H., Liu, H., Wang, B., et al. (2007) Liquid Submersion Culture Condition of Agaricus blazei. Journal of Hunan Agricultural University (Natural Sciences), 33, 475-479.
[9]	Zhang, W.J. (1994) Glycoconjugates Biochemical Research Techniques. Zhejiang University Press, Hangzhou.
[10]	You, M.L. (2007) The Food Chinese (Medicine) Uses the Fungus Fermentation Engineering Research Progress. Microbiology, 34, 327-331.
[11]	Lv, W.H., Cong, W. and Cai, Z.L. (2004) Effect of Sucrose Lactate and KH2PO4 on Nisin Production by L. lactis sp. Lactis. The Chinese Journal of Process Engineering, 4, 28-31.
[12]	Cai, W.C. and Yuan, H.J. (1982) Chemical Analysis of Biological Substances. Science Press, Beijing.