Extraction and Characterization of Starch Fractions of Five Phenotypes Pachyrhizus tuberosus (Lam.) Spreng

José Luis Ramírez Ascheri¹, Luz Haydee Bravo Zamudio², Carlos Wanderlei Piler Carvalho¹, Arturo Melendez Arevalo³, Lais Martins Fontoura³

¹Rheology Laboratory, Embrapa Food Technology, Avenida das Américas, Rio de Janeiro, Brazil.
²Agronomy and Veterinary Medicine Faculty, Brazilian University, Campus Universitário Darcy Ribeiro, Brasilia, Brazil.
³Graduate Program in Food Science and Technology, Federal Rural University of Rio de Janeiro, Seropédica, Brazil.

DOI: 10.4236/fns.2014.519200                

Abstract

Pachyrhizus tuberosus is a native plant of short life cycle found in South America riverside, which provides easy starch extraction from its tuberous roots. The aim of this study was to determine the physicochemical, rheological and functionality of the starch granules extracted from the roots of five phenotypes identified as V2, V3, V4, V6 and V7. Protein and ash content of all phenotypes were considerable high when compared to other root sources such as cassava varying from 4.35% to 7.43% and 1.58% to 2.49%, respectively, whereas lipid content was lower, between 0.29 and 0.49%. The starch granules were mostly circular and polygonal with varied sizes. The starch granules structural conformation showed cristallinity A type, normally for cereals. The maximum pasting viscosity at 95℃ ranged from 1644 cP (V7) to 2232 cP (V2). The initial temperature of pasting formation occurred at 69.4℃ for V2, 71.5℃ for V3, 87.9℃ for V4, 69.5℃ for V6 and 71.5℃ for V7. These values showed high variability within the phenotypes and generally high for roots and tubers starches. The maximum viscosity at 95℃ for V2, V3, V4, V6 and V7 were 2232, 2150, 1995, 2214 and 1644 cP, respectively. The viscosity curves showed low tendency to retrogradation. The thermal properties showed that the enthalpy of gelatinization varied from 8.91 J/g (V3) to 11.78 J/g (V2). The initial gelatinization temperature varied from 63.19℃ (V6) to 65.14℃ (V4). The swelling power at 90℃ ranged from 14.7% to 20.1% p/p and solubility from 10.3% (V2) to 27.2% (V7). It is concluded that Pachyrhizus tuberosus starch showed low retrogradation (1320 - 1560 cP) comparable to non-common native waxy starches, a feature which indicates the capability of using this natural and easy extraction starch source as gelling agent in certain manufactured food of undesirable retrogradation.

Keywords

Pachyrhizus tuberosus, Physicochemical, Rheological, Starch Properties

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	SØrensen, M., Grüneberg, W.J. and Ørting, B. (1997) Ahipa Pachyrhizus Ahipa (Wedd.) Parodi. In: Hermann, M. and Heller, J., Eds., Andean Roots and Tubers: Ahipa, Arracacha, Maca and Yacon. Promoting the Conservation and Use of Underutilized and Neglected Crops 21, Institute of Plant Genetics and Crop Plant Research, Gatersleben/International Plant Genetic Resources Institute, Rome, 13-73.
[2]	Bergthaller, W., Kjersting, H.J., Velasco, L. and Grüneberg, W.J. (2001) Andean Yam Bean (Pachyrhizus ahipa) Tubers as a New Source of a Legume Starch. Proceedings of the 4th European Conference on Grain Legumes, Cracow, 8-12 July 2001, European Association for Grain Legume Research (AEP), Paris, 392-393.
[3]	Tapia, C. and SØrensen, M. (2003) Morphological Characterization of the Genetic Variation Existing in a Neotropical Collection of Yam Bean, Pachyrhizus tuberosus (Lam.) Spreng. Genetic Resources and Crop Evolution, 50, 681-692. http://dx.doi.org/10.1023/A:1025028617948
[4]	Bermudez, J.J.H. (1997) Valorización de los Amiláceos no-cereales cultivados en los Países Andinos: Estudio de las Propiedades Fisicoquímicas y Funcionales de sus Almidones y de la Resistencia a Diferentes Tratamientos Estresantes. Thesis, Universidad de Bogotá, Colombia, 1-150.
[5]	Leonel, M. and Cereda, M.P. (2002) Caracterização físico-química de algumas tuberosas amiláceas. Ciência e Tecnologia de Alimentos, 22, 65-69. http://dx.doi.org/10.1590/S0101-20612002000100012
[6]	American Association of Cereal Chemists—AACC (2000) Approved Methods of the AACC. 10th Edition, Association of Official Analytical Chemists—AOAC, Saint Paul.
[7]	Association of Official Analytical Chemists (2000) Official Methods of Analysis. 13th Edition, Association of Official Analytical Chemists, Washington DC.
[8]	American Association of Cereal Chemists (1995) Approved Methods of the American Association of Cereal Chemists. 9th Edition, AACC, Saint Paul.
[9]	Leach, H.W., McCowen, L.D. and Schoch, T.J. (1959) Structure of the Starch Granule. I. Swelling and Solubility Patterns of Various Starches. Cereal Chemistry, 36, 534-544.
[10]	Franco, C.M.L., Wong, K.S., Yoo, S.H. and Jane, J.L. (2002) Structural and Functional Characteristics of Selected Soft Wheat Starches. Cereal Chemistry, 79, 243-248. http://dx.doi.org/10.1094/CCHEM.2002.79.2.243
[11]	da Rosa Zavareze, E., El Halal, S.L.M., Pereira, J.M., Radünz, A.L., Elias, M.C. and Dias, A.R.G. (2009) Caracterização química e rendimento de extração de amido de arroz com diferentes teores de amilose. Brazilian Journal of Food Technology. II SSA, 24-30. http://bjft.ital.sp.gov.br/artigos/especiais/especial_2009/v11_edesp_06.pdf
[12]	Carvalho, C.W.P. and Mitchell, J.R. (2001) Effect of Sucrose on Starch Conversion and Glass Transition of Nonexpanded Maize and Wheat Extrudates. Cereal Chemistry, St. Paul, 78, 342-348.
[13]	Leonel, M., Cereda, M. and Jackey, S. (1998) Processamento industrial de fécula de mandioca e batata doce-um estudo de caso. Ciência e Tecnologia de Alimentos, Campinas, 18, 343-345.
[14]	Leonel, M. and Cereda, M.P. (2000) Extração da fécula retida no resíduo fibroso do processo de produção de fécula de mandioca. Ciência e Tecnologia de Alimentos, Campinas, 20, 122-127.
[15]	Cereda, M.P. and Vilpoux, O. (2003) Tecnologia, uso e potencialidades de tuberosas amiláceas latinoamericanas. Série: Culturas de Tuberosas Amiláceas Latinoamericanas, Vol. 3, Fundação Cargill, São Paulo.
[16]	Nunes, L.B., de Jesus dos Santos, W. and Cruz, R.S. (2009) Rendimento de extração e caracterização química e funcional de féculas de mandioca da região do semi-árido baiano. Alimentos e Nutrição Araraquara, Araraquara, 20, 129-134.
[17]	López, O.V., Viña, S.Z., Pachas, A., Sisterna, M.N., Rohatsch, P.H., Mugridge, A., Fassola, H.E. and García, M.A. (2010) Composition and Food Properties of Pachyrhizus ahipa Roots and Starch. International Journal of Food Science & Technology, 45, 223-233. http://dx.doi.org/10.1111/j.1365-2621.2009.02125.x
[18]	Singh, N., Sandhu, K.S. and Kaur, M. (2005) Physicochemical Properties Including Granular Morphology, Amylose Content, Swelling and Solubility, Thermal and Pasting Properties of Starches from Normal, Waxy, High Amylose and Sugary Corn. Progress in Food Biopolymer Research, 1, 44-54.
[19]	Ciacco, F. and Cruz, R. (1982) Fabricação de amido e sua utilização. Série Tecnologia Agroindustrial, No. 7. Secretaria de Indústria e Comércio, Ciência e Tecnologia, São Paulo, 1-152.
[20]	Peroni, F.H.G., Rocha, T.S. and Franco, C.M.L. (2006) Some Structural and Physicochemical Characteristics of Tuber and Root Starches. Food Science and Technology International, 12, 505-513. http://dx.doi.org/10.1177/1082013206073045
[21]	Cereda, M.P., Franco, C.M.L., Daiuto, E.R., Demiate, I.M., Carvalho, L.J.C.B., Leonel, M., Vilpoux, O.F. and Sarmento, S.B.S. (2001) Propriedades gerais do Amido. Série: Culturas de Tuberosas Amiláceas latino-americanas, Vol. 1, Fundação Cargill, Campinas.
[22]	Ascheri, J.L.R. (1996) Characterization of Job’s Tears Starch. I. Extraction and Physical Properties of the Starch Granules. Alimentaria, 96, 97-100.
[23]	Weber, F.H., Collares-Queiroz, F.P. and Chang, Y.K. (2009) Caracterização físico-química, reológica, morfológica e térmica dos amidos de milho normal, ceroso e com alto teor de amilose. Ciência e Tecnologia de Alimentos, Campinas, 29, 748-753.
[24]	Tester, R.F. and Morrison, W.R. (1990) Swelling and Gelatinization of Cereal Starches. I. Effects of Amylopectin, Amylose and Lipids. American Association of Cereal Chemists, 67, 551-557. http://www.aaccnet.org/publications/cc/backissues/1990/documents/67_551.pdf
[25]	Ascheri, J.L.R. (1987) Extração e caracterização do amido de Adlay (Coix lacryma Jobi L.). Master Thesis in Food Science and Technology, Food Engineering Faculty, Universidade Estadual de Campinas-UNICAMP, Campinas, 1-130.
[26]	Felippe, Ó.R. and Andrade, C.T. (2012) Ternary Nanocomposites of Thermoplastic Starch and Maleated Polybutadiene. Quimica Nova, São Paulo, 35, 1146-1150.
[27]	Ascheri, J.L.R. (1996) Characterization of Job’s Tears Starch. II. Pasting Characteristics. Alimentaria, 96, 101-104.
[28]	Teixeira, M.A.V., Ciacco, C.F., Tavares, D.Q. and Bonezzi, A.N. (1998) Ocorrência e caracterização do amido resistente em amidos de milho e de banana. Ciência e Tecnologia de Alimentos, Campinas, 18, 246.