Changes in the organization of isozyme loci Me1 and Adh1 in-duced with triton X-100 in common wheat lines


The study of PCR-profiles of isozyme loci Me1 and Adh1 in common wheat lines obtained by means of treatment of initial cultivar Alem with detergent Tri-ton X-100 was carried out by the modified ISSR-amplification method. It was demonstrated that exposure to Triton X-100 causes changes of PCR-profiles of enzyme loci. The obtained data are suggestive of the role of chromatin and nuclear mem-brane interaction in structural-functional genome organization.

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Makhmudova, K. , Vinichenko, N. , Bogdanova, E. , Kirikovich, S. and Levites, E. (2011) Changes in the organization of isozyme loci Me1 and Adh1 in-duced with triton X-100 in common wheat lines. Advances in Bioscience and Biotechnology, 2, 128-131. doi: 10.4236/abb.2011.23020.

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The authors declare no conflicts of interest.


[1] Durrant, A. (1962) The environmental induction of heritable change in Linum. Heredity, 17, 27-61. doi:10.1038/hdy.1962.2
[2] Bogdanova, E.D. (1992) Wheat genetic variability induced by nicotinic acid and its derivatives. PH.D. Thesis, Institute of Cytology and Genetics SB RAS, No- vosibirsk.
[3] Bogdanova, E.D. (2003) Epigenetic variation induced in Triticum aestivum L. by nicotinic acid. Russian Journal of Genetics, 39, 1221-1227. doi:10.1023/A:1025775101592
[4] Makhmudova, K.Kh. (2007) Inheritance of epigenetic alterations in common wheat (Triticum aestivum L.). Thesis of Candidate of Sciences, Kazakh National Uni- versity, Almaty.
[5] Makhmudova, K.Kh., Bogdanova E.D. and Levites E.V. (2009) Triton X-100 induces heritable changes of mor- phological characters in Triticum aestivum L. Russian Journal of Genetics, 45, 495-498. doi:10.1134/S1022795409040152
[6] Kirikovich, S.S. and Levites, E.V. (2009) Effect of epimutagene Triton X-100 on morphological traits in sugar beet (Beta vulgaris L.). Sugar Tech, 11, 307-310.
[7] Durrant, A. and Timmis, J.N. (1973) Genetic control of environmentally induced changes in Linum. Heredity, 30, 369-379. doi:10.1038/hdy.1973.45
[8] Cullis, C.A. (1973) DNA differences between flax genotrophs. Nature, 243, 515-516. doi:10.1038/243515a0
[9] Richards, E. (1997) DNA methylation and plant develop- ment. Trends in Genetics, 13, 319-323. doi:10.1016/S0168-9525(97)01199-2
[10] Vanyushin, B.F. and Kirnos, M.D. (1988) DNA meth- ylation in plants. Gene, 74, 117-121.
[11] Lusser, A. (2002) Acetylated, methylated, remodeled: chromatin states for gene regulation. Current Opinion in Plant Biology, 5, 437-443. doi:10.1016/S1369-5266(02)00287-X
[12] Pfluger, J. and Wagner, D. (2007) Histone modifications and dynamic regulation of genome accessibility in plants. Current Opinion in Plant Biology, 10, 645-652. doi:10.1016/j.pbi.2007.07.013
[13] Cullis, C.A. (1981) Environmental induction of heritable changes in flax: defined environments inducing changes in r-DNA and peroxidase isozyme band pattern. Heredity, 47, 87-94. doi:10.1038/hdy.1981.61
[14] Cullis, C.A. (2005) Mechanisms and control of rapid genomic changes in flax. Annuals of Botany, 95, 201- 206. doi:10.1093/aob/mci013
[15] Mosolov, A.N. (1972) New approach to decide a problem of spatial disposition of chromosomes in inter- phase nucleus (polar model of interphase nucleus). Cytology, 14, 542-552.
[16] Taddei, A., Hediger, F., Neumann, F.R. and Gasser, S.M. (2004). The function of nuclear architecture: a genetic approach. Annual Review of Genetics, 38, 305-345. doi:10.1146/annurev.genet.37.110801.142705
[17] Vinichenko, N.A., Kirikovich, S.S. and Levites E.V. (2006) The genetic instability of the Adh1 locus alleles in sugar beet agamospermous progeny. Sugar Tech, 8, 288-291. doi:10.1007/BF02943570
[18] Vinichenko, N.A., Kirikovich, S.S. and Levites, E.V (2008) Polymorphism of PCR profiles and expression of alleles at the locus Adh1 in agamospermous progeny of sugar beet Beta vulgaris L. Russian Journal of Genetics, 44, 1092-1095. doi:10.1134/S1022795408090123
[19] Vinichenko, N.A., Kirikovich, S.S. and Levites, E.V. (2007) Tissue distinctions in the organization of sugar beet locus Adh1. Achievements and Problems of Genetics, Breeding and Biotechnology, 2, 247-251.
[20] Vinichenko, N.A, Bogdanova, E.D, Makhmudova, K.Kh., Kirikovich, S.S. and Levites, E.V. (2010) Molecular differences in genotroph forms of common wheat (Tri- ticum aestivum L.) and their initial cultivars. Advances in Bioscience and Biotechnology, 1, 162-166. doi:10.4236/abb.2010.13022
[21] Doyle, J.J. and Doyle, J.L. (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phyto- chemical Bulletin, 19, 11-15.
[22] Scandalios, J.G. (1967) Genetic control of alcohol dehy- drogenase isozymes in maize. Biochemical Genetics, 1, 1-9. doi:10.1007/BF00487731
[23] Famiani, F., Walker, R.P., László, T., Chen, Z.H., Proietti, P. and Leegood, R.C. (2000) An immunohisto- chemical study of the compartmentation of metabolism during the development of grape (Vitis vinifera L.) berries. Journal of Experimental Botany, 51, 675-683. doi:10.1093/jexbot/51.345.675

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