Membrane Proteins as Potential Colon Cancer Biomarkers: Verification of 4 Candidates from a Secretome Dataset
Sum-Fu Chiang1,2, Ming-Hung Tsai3, Reiping Tang1,4, Ling-Ling Hsieh5, Jy-Ming Chiang1, Chien-Yuh Yeh1, Pao-Shiu Hsieh1, Wen-Sy Tsai1, Ya-Ping Liu6, Ying Liang6, Jinn-Shiun Chen1*, Jau-Song Yu7*
1Division of Colon and Rectal Surgery, Chang Gung Memorial Hospital, New Taipei City.
2Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan.
3Graduate Institute of Biomedical Sciences, Chang Gung University, Taoyuan.
4College of Medicine, Chang Gung University, New Taipei City.
5Department of Public Health, Chang Gung University, Taoyuan.
6Pathology Core of the Chang Gung Molecular Medicine Research Center, Taoyuan.
7Department of Biochemistry and Molecular Biology, Chang Gung University, Taoyuan.
DOI: 10.4236/ss.2014.510067   PDF    HTML   XML   4,525 Downloads   5,729 Views   Citations


Colorectal cancer (CRC) is an important health issue in Taiwan. There were over ten thousand newly diagnosed CRC patients each year. The outcome of late stage CRC still remains to be improved, and tumor markers are expected to improve CRC detection and management. From a colorectal cancer cell secretome database, we chose four proteins as candidates for clinical verification, including tumor-associated calcium signal transducer 2 (TROP2, TACSTD2), transmembrane 9 superfamily member 2 (TM9SF2), and tetraspanin-6 (TSPAN6), and tumor necrosis factor receptor superfamily member 16 (NGFR). Different groups of 30 CRC patients’ tissue samples collected from Chang Gung Memorial Hospital were analyzed by immunohistochemistry (IHC) for the four proteins, and the results were scored by pathologist. For all the four candidate proteins, marked differences of IHC score existed between tumor and adjacent non-tumor counterpart. However, there were only trends between higher protein expression levels and worse outcome. Three proteins (TROP2, TM9SF2 and NGFR) had trends between higher tissue expression and tumor stage or lymph node metastasis. Our study revealed that tissue expression of four proteins (TROP2, TM9SF2, TSPAN6, and NGFR) was markedly different between tumor and adjacent non-tumor counterparts. Overexpression of all these four proteins showed some trends with poorer survival.

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Chiang, S. , Tsai, M. , Tang, R. , Hsieh, L. , Chiang, J. , Yeh, C. , Hsieh, P. , Tsai, W. , Liu, Y. , Liang, Y. , Chen, J. and Yu, J. (2014) Membrane Proteins as Potential Colon Cancer Biomarkers: Verification of 4 Candidates from a Secretome Dataset. Surgical Science, 5, 418-438. doi: 10.4236/ss.2014.510067.

Conflicts of Interest

The authors declare no conflicts of interest.


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