Escherichia coli Population-Based Study in Pediatric Crohn’s Disease

Serena Schippa, Valentina Totino^*, Massimiliano Marazzato, Maria Stefania Lepanto, Floriana Santangelo, Marta Aleandri, Antonella Gagliardi, Catia Longhi, Fabrizio Pantanella, Valerio Iebba, Maria Pia Conte

Microbiology Section, Department of Public Health and Infectious Diseases, “Sapienza” University of Rome, Rome, Italy.

DOI: 10.4236/aim.2014.413098                

Abstract

Escherichia coli is assumed to be involved in inflammatory bowel disease (IBD) by many authors. The present Short Report was aimed at analyzing E. coli population isolated from ileal samples collected from 4 CD and 3 non-CD (control group) diagnosed pediatric patients. A total of 539 mucosa-associated E. coli strains were characterized by: Random Amplified Polymorphic DNA (RAPD), adhesive and virulence factors, and for their phylogenetic groups. A significant separation between RAPD profiles of the two CD and non-CD cohorts (P < 0.0001), along with a significant reduction of intra-species genomic variability in E. coli populations isolated from CD group (P < 0.0001) was found as assessed by Dice index, indicating a different selective pressure in CD intestinal habitat. A predominance of phylogenetic group A was found in control subjects (P < 0.0001). Results on adhesive and virulence factors evidenced peculiar genes significantly related to CD E. coli populations (K1, IbeA) (P < 0.0001) and to total DNA from biopsy specimens K1 (P < 0.0001). Results suggest that among E. coli population, particular variants may be favorite in the intestinal habitat of CD pediatric patients. These genotype variants could represent the genetic background that, via evolutionary phenomena driven by a persistent inflammatory state, may evolve in Adherent Invasive Escherichia coli (AIEC) like strains found in adult CD patients.

Keywords

Escherichia coli, Mucosa-Associated, Crohn’s Disease

Conflicts of Interest

The authors declare no conflicts of interest.

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