A Polymerase Chain Reaction Based DNA Fingerprinting Technique: Amplified Fragment Length Polymorphism for Molecular Typing of Perchlorate Reducing Bacteria

Anita Shete^1*, Vikram Ghole², Manju Raina³, Bikash Aich¹

¹Aavanira Biotech Pvt. Ltd., Pune, India.
²Department of Biochemistry, University of Pune, Pune, India.
³Ministry of Environment & Forests, New Delhi, India.

DOI: 10.4236/jep.2014.512109                

Abstract

Genetic profiling of environmentally important organisms is very essential for easy identification of biodegrading bacteria. In the previous study, we have reported the perchlorate biodegrading bacteria and characterized them by biochemical analysis and 16 S sequencing. We have observed a very similar isolates of Arthrobacter (Actinobacteria) degrading 4.1 mM and 4.7 mM of ammonium perchlorate [1]. In this study, we report PCR based DNA fingerprinting technique to generate the genomic signature of these closely related group of Arthrobacter species. This study also effectively generates unique genomic signature for each of these isolates that has potential for use in molecular monitoring as well as for tracking genomic variation and rearrangements.

Keywords

AFLP—Amplified Fragment Length Polymorphism, Arthrobacter, Ammonium Perchlorate, Bio-Degradation

Conflicts of Interest

The authors declare no conflicts of interest.

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