Scanning Electron Microscopy Observation of Adhesion Properties of Bifidobacterium longum W11 and Chromatographic Analysis of Its Exopolysaccaride


Bifidobacterium spp. can produce cell-bound or released exopolysaccharide (EPS) that is a beneficial trait mediating commensal-host interactions. However differences in the physico-chemical characteristics of EPS produced by different strains of Bifidobacterium spp. are determinant in adhesion ability and modulation of immune response. The aim of this study was to investigate the in vitro adhesion characteristics of Bifidobacterium longum W11 to intestinal epithelial cell-line HT-29, by Scanning Electron Microscopy (SEM), and chemical characteristics of its exopolysaccharide using Thin-Layer Chromatography (TLC) analysis. SEM observation showed a good adhesion of B. longum W11 to the HT-29 monolayer that could be increased by the production of exocellular polymers formation. TLC analysis of the purified and hydrolyzed EPS showed that the cell-surface and extracellular polysaccharide were composed mainly of fructose and glucose. Moreover, other sugars were present in smaller quantities. Information from this study on physico-chemical characteristics of EPS of B. longum W11 could contribute to understanding the physiology of bifidobacteria and their interaction with the host.

Share and Cite:

Inturri, R. , Stivala, A. , Sinatra, F. , Morrone, R. and Blandino, G. (2014) Scanning Electron Microscopy Observation of Adhesion Properties of Bifidobacterium longum W11 and Chromatographic Analysis of Its Exopolysaccaride. Food and Nutrition Sciences, 5, 1787-1792. doi: 10.4236/fns.2014.518192.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Saarela, M., Mogensen, G. and Fondén, R. (2000) Probiotic Bacteria: Safety, Functional and Technological Properties. Journal of Biotecnology, 84, 197-215.
[2] Medina, M., Izquierdo,E., Ennahar, S. and Sanz Y. (2007) Differential Immunomodulatory Properties of Bifidobacterium logum Strains: Relevance to Probiotic Selection and Clinical Applications. Clinical and Experimental Immunology, 150, 531-538.
[3] Blandino, G., Fazio, D. and Di Marco, R. (2008) Probiotics: Overview of Microbiological and Immunological Characteristics. Expert Review of Anti-Infective Therapy, 64, 497-508.
[4] Serafini, F., Strati, F., Ruas-Madiedo, P., Turroni, F., Foroni, E., Durati, S., Milano, F., Perotti, A., Vioppiani, A., Guglielmetti, S., Buschini, A., Mergolles, A., van Sinderen, D. and Ventura M. (2013) Evaluation of Adhesion Properties and Antibacterial Activities of the Infant Gut Commensal. Bifidobacterium bifidum PRL2010. Anaerobe, 21, 9-17.
[5] Ruas-Madiedo, P., Hugenholtz, J. and Zoon, P. (2002) An Overview of the Functionality of Exopolysaccharides Produced Bylacticacidbacteria. International Dairy Journal, 12, 163-171.
[6] Fanning, S., Hall, L.J., Cronin, M., Zomer, A., MacSharry, J., Goulding, D., O’Connel Motherway, M., Shanahan, F., Nally, K., Dougan, G. and Van Sinderen, D. (2012) Bifidobacterial Surface-Exopolisaccharide Facilitates Commensal-Host Interaction through Immune Modulation and Pathongen Protection. PNAS, 109, 2108-2113.
[7] López, P., Monteserín, D.C., Gueimonde, M., de losReyes-Gavilán, C.G., Margolles, A., Suárez, A. and Ruas-Madiedo, P. (2012) Exopolysaccharide-Production with Human Cells. Food Research International, 46, 99-107.
[8] Hidalgo-Cantabrana, C., Nikolic, M., López, P., Suárez, A., Miljkovic, M., Kojic, M., Margolles, A., Golic, N., RuasMadiedo, P. (2014) Exopolysaccharide-Producing Bifidobacterium animalis subsp. lactis Strains and Their Polymers Elicit Different Responses on Immune Cells from Blood and Gut Associated Lymphoid Tissue. Anaerobe, 26, 24-30.
[9] Ali, Q.S., Farid, A.J., Kabeir, B.M., Zamberi, S., Shuhaimi, M., Ghazali, H.M. and Yazid, A.M. (2009) Adhesion Properties of Bifidobacterium pseudocalenulatum G4 and Bifidobacterium longum BB 536 on HT29 Human Epithelial Cell Line and Different Times and pH. World Academy of Science, Engineering and Technology, 49, 149-153.
[10] Ruas-Madiedo, P., Gueimonde, M., Margolles, A., de los Reyes-Gavilán, C.G. and Salminen, S. (2006) Exopolysaccharides Produced by Probiotic Strains Modify the Adhesion of Probiotics and Enteropathogens to Human Intestinal Mucus. Journal of Food Protection, 69, 2011-2015.
[11] Dubois, M., Gilles, J.K., Hamilton, P.A., Rebers, P.A. and Smith F. (1956) Colorimetric Method for Determination of Sugars. Analytical chemistry, 28, 350-356.
[12] Yang, Z., Li, S., Zhang, X., Zeng, X., Li, D., Zhao, Y. and Zhang, J. (2010) Capsular and Slime-Polysaccharide Production by Lactobacillus rhamnosus JAAS8 Isolated from Chinese Sauerkraut: Potential Application in Fermented Milk Products. Journal of Bioscience and Bioengineering, 110, 53-57.
[13] Leivers, S., Hidalgo-Cantabrana, C., Robinson, G., Margolles, A., Ruas-Madiedo, P. and Laws, A.P. (2011) Structure of the High Molecular Weight Exopolysaccharide Producedby Bifidobacterium animalis subsp. lactis IPLA-R1 and Sequence Analysis of Its Putative Eps Cluster. Carbohydrate Research, 346, 2710-2717.
[14] Fanning, S., Hall, L.J., van Sinderen, D. and Hidalgo-Cantabrana, C. (2014) Bifidobacteriumbreve UCC2003 Surface Exopolysaccharide Production Is a Beneficial Trait Mediating Commensal-Host Interaction through Immune Modulation and Pathogen Protection. Gut Microbes, 3, 420-425.
[15] Hidalgo-Cantabrana, C., Sánchez, B., Milani, C., Ventura, M., Margolles, A. and Ruas-Madiedo, P. (2014) Genomic Overview and Biological Functions of Exopolysaccharide Biosynthesis in Bifidobacterium spp. Applied and Environmental Microbiology, 80, 9-18.

Copyright © 2023 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.