Petit-Suisse Cheese Production with Addition of Probiotic Lactobacillus casei

Ludmilla Santana Soares e Barros, Nelson De Carvalho Delfino

Universidade Federal do Recôncavo da Bahia, Centro de Ciências Agrárias, Ambientais e Biológicas, Laboratório de Investigação Analítica de Alimentos e de água, Rua Rui Barbosa, 710, Centro. Cruz das Almas, Brazil.

DOI: 10.4236/fns.2014.518189                

Abstract

Growing concern to increase life expectancy has promoted several studies in the field of nutrition, especially those on food and their effects on the human body. Assays have been performed to improve their nutritional quality. In fact, there is considerable interest in encouraging development of new ingredients, with the innovation in food products and the establishment of new market niches for these ingredients. The global market for functional foods is growing and always envisages new products with functional technological and physiological characteristics. The petitsuisse cheese is a product of high nutritional value, rich in calcium, phosphorus and fat-soluble vitamins, with a high percentage of milk proteins, coupled to excellent digestion and assimilation by the human body. Current assay describes the production of a petit-suisse cheese to which was added the probiotic Lactobacillus casei BGP 93, and determines the physical, chemical and microbiological viability of lactobacilli in cheese during storage period. Levels of protein, fat in the dry extract, moisture and pH were analyzed for physical and chemical parameters; likewise, detection of coliforms, Staphylococcus, Salmonella, Listeria monocytogenes, and yeasts and molds was performed for microbiological parameters; monitoring of the viability of Lactobacillus casei BGP 93 during its life time was conducted. The petit-suisse cheese proved to be a good vehicle for the addition of probiotic microorganism. Lactobacillus casei remained viable during shelf life even with decreasing pH, and may be considered a functional product during 30 days of storage.

Keywords

Cheese, Probiotic, Lactobacillus casei

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Raizel, R., Santini, E., Koppert, A.M. and Filho, A.D.R. (2011) Efeitos do consumo de probióticos, prebióticos e simbióticos para o organismo humano. Revista Ciência & Saúde, 4, 66-74.
[2]	Souza, J.C.B., Guergoletto, K.B., Garcia, S. and Sivieri, K. (2011) Viabilidade da adição de Lactobacillus casei (LC-1) protegido com trealose e goma acácia em sorvetes. Alimentos e Nutrição, 22, 231-237.
[3]	Brasil (2000) Ministry of Agriculture, Livestock and Supply. Instruction, Paragraph .53 of December 29, 2000. Brasilia.
[4]	Gambelli, L., Manzi P., Panfili, V. and Pizzoferrato, L. (1999) Constituents of Nutritional Relevance in Fermentaded Milk Products Commercialised in Italy. Food Chemistry, 66, 353-358. http://dx.doi.org/10.1016/S0308-8146(99)00075-8
[5]	Van Dender, A.G.F. and Panfili, V. (1985) Adaptação de tecnologia de fabricação e termização do queijo quark. Revista do Instituto de Laticínios Candido Tostes, 40, 33-53.
[6]	Kelly, A.L. and O’Donnell, H.J. (1998) Composition, Gel Properties and Microstruture of Quarg as Affected by Processing Parameters and Milk Quality. International Dairy Journal, 8, 295-301. http://dx.doi.org/10.1016/S0958-6946(98)00084-3
[7]	Albuquerque, L.C. (2002) Queijos no mundo: Origem e Tecnologia. Juiz de Fora: CT/ILCT/EPAMIG.
[8]	Brasil (2006) Ministry of Agriculture, Livestock and Supply. Instruction, n.68 of December 12, 2006. Brasilia.
[9]	Brasil (2003) Ministry of Agriculture, Livestock and Supply. Instruction n.62 of August 26, 2003. Brasilia.
[10]	Banzatto, D.V. and Kronka, S. do N. (1992) Experimentação agrícola. Jaboticabal: FCAV/UNESP.
[11]	Brazil (1996) Ministry of Agriculture, Livestock and Supply. Ordinance No. 146 of 07 Sea 1996. Official Gazette of the Federative Republic of Brazil, Brasilia.
[12]	Maruyama, L.Y., Cardarelli, H.R., Buriti, F.C.A. and Saad, S.M.I. (2006) Textura instrumental de queijo petit-suisse potencialmente probiótico: Influencia de diferentes combinações de gomas. Ciência e Tecnologia de Alimentos, 26, 386-393. http://dx.doi.org/10.1590/S0101-20612006000200022
[13]	Souza, V.R., Carneiro, J.D.S., Pinheiro, A.C.M., Pinto, S.M., Carvalho, L.P. and Menezes, C.C. (2010) Elaboração de queijo petit-suisse sabor morango de baixo valor calórico. Revista do Instituto de Laticínios Candido Tostes, 65, 49-58.
[14]	Alves, C.C.C. (2010) Comportamento da Escherichia coli em queijo minas frescal elaborado com utilização de Lactobacillus acidophilus e de acidificação direta com ácido lático. Master’s Dissertation, Universidade Federal Fluminense, Rio de Janeiro.
[15]	Veiga, P.G., Cunha, R.L., Viotto, W.H. and Petenate, A.J. (2000) Caracterização química, reológica e aceitação sensorial do queijo petit-suisse brasileiro. Ciência e Tecnologia de Alimentos, 20. http://dx.doi.org/10.1590/S0101-20612000000300012
[16]	Veiga, P.G. and Viotto, W.H. (2001) Fabricação de queijo petit-suisse por ultrafiltração do leite coagulado. Efeito do tratamento térmico do leite no desempenho da membrana. Ciência e Tecnologia de Alimentos, 21, 267-272. http://dx.doi.org/10.1590/S0101-20612001000300003
[17]	Boatto, D.A., Mesomo, M.C., Madrona, G.S., Branco, I.G. and Matumoto-Pintro, P.T. (2010) Desenvolvimento e caracterização de queijo tipo petit-suisse de soja comum e de soja livre de lipoxigenase, enriquecidos com cálcio. Ciência e Tecnologia de Alimentos, 30, 766-770. http://dx.doi.org/10.1590/S0101-20612010000300031
[18]	Buriti, F.C.A., Cardarelli, H.R. and Saad, S.N.I. (2008) Textura instrumental e avaliação sensorial de queijo fresco cremoso simbiótico: Implicações da adição de Lactobacillus paracasei e inulina. Revista Brasileira de Ciências Farmacêuticas, 44, 75-84. http://dx.doi.org/10.1590/S1516-93322008000100009
[19]	Ong, L., Henriksson, A. and Shah, N.P. (2007) Chemical Analysis and Sensory Evaluation of Cheddar Cheese Produced with Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus paracasei or Bifidobacterium sp. International Dairy Journal, 17, 937-945. http://dx.doi.org/10.1016/j.idairyj.2007.01.002
[20]	Prudencio, I.D., Prudêncio, E.S., Gris, E.F., Tomazi, T., Marilde, T. and Bordignon-Luiz, T. (2008) Petit-suisse Manufactured with Cheese Whey Retentate and Application of Betalains and Anthocyanins. LWT—Food Science and Technology, 41, 905-910.
[21]	Ong, L. and Shah, N.P. (2009) Probiotic Cheddar Cheese: Influence of Ripening Temperatures on Survival of Probiotic Microorganisms, Cheese Composition and Organic Acid Profiles. LWT—Food Science and Technology, 42, 1260- 1268.
[22]	Ong, L., Henriksson, A. and Shah, N.P. (2007) Proteolytic Pattern and Organic Acid Profiles of Probiotic Cheddar Cheese as Influenced by Probiotic Strains of Lactobacillus acidophilus, Lactobacillus paracasei, Lactobacillus casei or Bifidobacterium sp. International Dairy Journal, 17, 67-78. http://dx.doi.org/10.1016/j.idairyj.2005.12.009
[23]	Buriti, F.C.A. (2005) Desenvolvimento de queijo fresco cremoso simbiótico. Master’s Dissertation, Universidade de São Paulo, Faculdade de Ciência Farmacêutica, São Paulo.
[24]	Dabiza, N. and El-Deb, K. (2007) Biochemical Evaluation and Microbial Quality of Ras Cheese Supplemented with Probiotic Strains. Polish Journal of Food and Nutrition Sciences, 5, 295-300.
[25]	Osman, M.M. and Abbas, F.M. (2001) Fate of Lactobacillus acidophilus La-5 and Bifidobacterium latis Bb-12 in Probiotic Ras Cheese. Proceeding of the 8th Egyptian Conference for Dairy Science & Technology, 3, 653-604.
[26]	Ravila, R.R. and Shah, N.P. (1998) Selective Enumeration of Lactobacillus casei from Yoghurts and Fermented Milk. Biotechnology Techniques, 12, 819-822. http://dx.doi.org/10.1023/A:1008829004888
[27]	Shah, N.P. (2000) Probiotic Bacteria: Selective Enumeration and Survival in Dairy Foods. Journal of Dairy Science, 83, 894-907. http://dx.doi.org/10.3168/jds.S0022-0302(00)74953-8
[28]	Shah, N.P. (2001) Functional Foods, Probiotics and Prebiotics. Food Technology, 55, 46-53.
[29]	Buriti, F.C.A., Rocha, J.S. and Saad, S.M.I. (2005) Incorporation of Lactobacillus Acidophilus in Minas Fresh Cheese and Its Implications for Textural and Sensorial Properties during Storage. International Dairy Journal, 12, 1279-1288. http://dx.doi.org/10.1016/j.idairyj.2004.12.011
[30]	Ribeiro, E.P., Simões, L.G. and Jurkiewicz, C.H. (2009) Desenvolvimento de queijo minas frescal adicionado de Lactobacillus acidophilus produzido a partir de retentados de ultrafiltração. Ciência e Tecnologia de Alimentos, 29, 19-23. http://dx.doi.org/10.1590/S0101-20612009000100004
[31]	Vinderola, C.G., Prosello, W., Ghiberto, D. and Reinheimer, J.A. (2000) Viability of Probiotic (Bifidobacterium, Lactobacillus acidophillus, Lactobacillus casei) and Nonprobiotic Microflora in Argentinian Fresco Cheese. Journal of Dairy Science, 83, 1905-1911. http://dx.doi.org/10.3168/jds.S0022-0302(00)75065-X
[32]	Ong, L., Henriksson, A. and Shah, N.P. (2006) Development of Probiotic Cheddar Cheese Containing Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus paracasei and Bifidobacterium spp. and the Influence of These Bacteria on Proteolytic Patterns and Production of Organic Acid. International Dairy Journal, 16, 446-456. http://dx.doi.org/10.1016/j.idairyj.2005.05.008
[33]	Brazil (2002) Ministry of Health National Health Surveillance Agency (ANVISA). RDC Resolution No. 2 of January 7, 2002.
[34]	Vasiljevic, T. and Saha, N.P. (2008) Probiótics—From Metchnikoff to Bioactives. International Dairy Journal, 18, 714-728. http://dx.doi.org/10.1016/j.idairyj.2008.03.004