Share This Article:

Sweet Potato Starch as a Carbon Source for Growth and Glucoamylase Production from Aspergillus niger

Abstract Full-Text HTML Download Download as PDF (Size:2687KB) PP. 788-795
DOI: 10.4236/aim.2014.412087    3,375 Downloads   4,009 Views   Citations

ABSTRACT

A mesophilic strain of Aspergillus niger isolated from cassava effluent samples produced extracellular glucoamylase in submerged culture containing 2% (w/v) soluble or sweet potato starch. On soluble starch medium the maximum glucoamylase activity in the culture filtrate was 9.40 U/mg compared to 8.24 U/mg on sweet potato starch culture filtrate. The mycelial dry weight for both media was 494 and 418 mg respectively. The maximum glucoamylase activity was obtained at a growth temperature of 40°C and pH 4.5. The implication is that the bioprocess of utilizing sweet potato starch in the culture is attractive due to its relatively cheaper availability in Nigeria, making it even more favorable when economics is considered.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Ubalua, A. (2014) Sweet Potato Starch as a Carbon Source for Growth and Glucoamylase Production from Aspergillus niger. Advances in Microbiology, 4, 788-795. doi: 10.4236/aim.2014.412087.

References

[1] Frost, G.M. and Moss, D.A. (1987) Production of Enzymes by Fermentation. In: Rehm, H.J., Reed, G., Eds., Biotechnology, Vol. 7a, VCH, Weinheim, 65-102.
[2] Kluyver, A.J. and Perquin, L.H.C. (1932) Zur Methodik der Schimmel-stoffwechseluntersuchung. Biochem Z, 266, 68-81.
[3] Schuster, E., Dunn-Coleman, N., Frisvad, J.C. and van Dijck, P.W.M. (2002) On the Safety of Aspergillus niger—A Review. Applied Microbiology and Biotechnology, 59, 426-435.
http://dx.doi.org/10.1007/s00253-002-1032-6
[4] Castellonos, O.F., Sinitsyn, A.P. and Vlasenko, E.Y. (1995) Comparative Evaluation of Hydrolytic Efficiency toward Microcrystalline Cellulose of Penicillium and Trichoderma Cellulases. Bioresource Technology, 52, 119-124. http://dx.doi.org/10.1016/0960-8524(95)00011-3
[5] Fogarty, W.M. and Kelly, C.T. (1980) Amylase, Amyloglucosidase and Related Glucanases. In: Rose, A.H., Ed., Microbial Enzymes and Bioconversions, Vol. 5, Academic Press, London, 770-778.
[6] Chu, W.-H. (2007) Optimization of Extracellular Alkaline Protease Production from Ciacco, Fabricacao de amido e sua utilizacao. Secretaria da Industria, Comercio, Ciencia e Tecnologia, Sao Paulo.
[7] Barnett, H.L. and Hunter, B.B. (1972) Illustrated Genera of Imperfect Fungi. 3rd Edition, Burgess Publishing Co., Minneapolis, 241 p.
[8] Nelson, S. (1944) A Photometric Adaptation of the Somogyi Method for the Determination of Glucose. The Journal of Biological Chemistry, 153, 375-380.
[9] Sani, A., Awe, F.A. and Akinyanju, J.A. (1992) Amylase Synthesis in Aspergillus flavus and Aspergillus niger on Cassava Peel. Journal of Industrial Microbiology Biotechnology, 10, 55-59.
http://dx.doi.org/10.1007/BF01583634
[10] Uguru, G.C., Akinyanju, J.A. and Sani, A. (1997) The Use of Yam Peel for Growth of Locally Isolated Aspergillus niger and Amylase Production. Enzyme and Microbial Technology, 21, 48-51.
http://dx.doi.org/10.1016/S0141-0229(96)00225-6
[11] Gupta, R., Gigras, P., Mohapatra, H., Goswami, K.V. and Chauhan, B. (2003) Microbial α-Amylases: A Biotechnological Perspective. Process Biochemistry, 10, 1-18.
[12] Fogarty, W.M. (1983) Microbial Amylases. In: Fogarty, W.M., Ed., Microbial Enzyme and Biotech, Applied Science Publishers, London, 1-90
[13] Carlsen, M., Spohr, A.B., Nielsen, J. and Villadsen. J. (1996) Morphology and Physiology of an α-Amylase Producing Strain of Aspergillus oryzae during Batch Cultivations. Biotechnology and Bioengineering, 49, 266-276.
http://dx.doi.org/10.1002/(SICI)1097-0290(19960205)49:3%3C266::AID-BIT4%3E3.0.CO;2-I
[14] Djekrif-Dakhmouche, S., Gheribi-Aoulmi, Z., Merihi, Z. and Bennamoun, L. (2005) Application of a Statistical Design to the Optimization of Culture Medium for α-Amylase Production by Aspergilus niger ATCC 16404 Grown on Orange Waste Powder. Journal of Food Engineering, 73, 190-197.
http://dx.doi.org/10.1016/j.jfoodeng.2005.01.021
[15] Oguntimein, G.B. (1993) Growth and Amylase Production by Bacillus licheniformi Isolated from Cassava Processing Waste. Nigerian Food Journal, 11, 60-67.
[16] Cordeira, C.A.M., Martins, M.L.L. and Luciano, A.B. (2002) Production and Properties of α-Amylase from Thermophilic Bacillus sp. Brazilian Journal of Microbiology, 33.
[17] Gomes, E., de Souza, S.R., Grandi, R.P. and Da Silva, R. (2005) Production of Thermostable Glucoamylase by a Newly Isolated Aspergillus flavus A1.1 and Thermomyces lanuginosus A 13.37. Brazilian Journal of Microbiology, 36, 75-82.

  
comments powered by Disqus

Copyright © 2019 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.