Ameliorative Potential of Mesenchymal Stem Cells and Hematopoietic Stem Cells against CCl₄ Induced Chromosomal Abnormalities in Experimental Rat Liver Fibrosis

Rokaya H. Ahmed¹, Nagwa K. Roshdy², Hayam E. Saleh¹, Mohammed I. Aref³, Nermeen M. Hassan², Noura R. Mohammed¹

¹Zoology Department, Women’s College, Ain Shams University, Cairo, Egypt.
²Biochemistry Department, Faculty of Medicine, Cairo University, Giza, Egypt.
³Immunology Department, Faculty of Medicine, Al Azhar University, Cairo, Egypt.

DOI: 10.4236/scd.2014.44010                

Abstract

Background and Objective: The liver is responsible for metabolism and detoxification of the most of components that enter the body. Once the liver became injured, its efficient treatment with famous chemical drugs was limited. Therefore, interest concerned the use of alternative medicines for the treatment of hepatic disease has been arisen. The present study was aimed to investigate the therapeutic effect of the two types of stem cells against chromosomal aberrations in bone marrow (BM) cells of rats treated with carbon tetrachloride (CCl₄). Design and Method: BM-derived mesenchymal stem cells (MSCs) were isolated and propagated in culture for 2 weeks and were characterized morphologically. Human umbilical cord blood (UCB) cells were obtained after full-term caesarean delivery from healthy donors. Low-density mononuclear cells were separated over Ficoll-Paque, and CD34⁺ hematopoietic cells were isolated using a magnetic cell sorter. Rats were divided into 4 groups: control, CCl₄, CCl₄ plus MSC, and CCl₄ plus CD34⁺. Liver tissue was examined histopathologically for all groups. Results: The results of the present study indicated a significant increase (p ＜ 0.05) in the number of metaphases with different types of chromosomal aberrations in CCl₄ group. Treatment of the animals with BM-MSCs and UCB-CD34⁺ cells improved both genotoxicity and histopathological changes induced by CCl₄.

Keywords

Cytogenetic and Stem Cells, CCl4 and Chromosomal Aberrations

Conflicts of Interest

The authors declare no conflicts of interest.

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