Adherence of a Clinical Strain of Mycoplasma fermentans to Human Cultured Epithelial Cells

Mónica Rosales-Pérez, Silvia Giono-Cerezo, Jorge Girón, Antonio Yáñez, Lilia Cedillo

Centro de Detección Biomolecular, VI.E.P., B.U.A.P. Puebla, Pue. México.
Escuela Nacional de Ciencias Biológicas, I.P.N, México, D. F. México.
Facultad de Agrobiología, Universidad Autónoma de Tlaxcala, México.
Facultad de Estomatología, B.U.A.P. Puebla, México.

DOI: 10.4236/aim.2014.411079                

Abstract

This study compares the kinetics of adherence of both the prototype strain PG-18 and the recently clinically isolated strain P-140 of Mycoplasma fermentans to HEp-2 cells. M. fermentans may induce respiratory and genitourinary tract infections. We investigated the presence of putative adhesins from strain P-140. A surface protein of 47 kDa, labeled SP47, isolated from M. fermentans P-140, seems to be involved in acterial adherence on HEp-2 cells; this study could allow helping understand the interaction between mycoplasmas and their hosts. Anti-SP47 antibodies inhibited the formation of bacterial clusters and adherence to cultured cells, quantified by ELISA. M. fermentans P-140 was more efficient at adhering to cultured HEp-2 cells than PG-18.

Keywords

Mycoplasma fermentans, Mycoplasmas, Adherence, Pathogenesis

Conflicts of Interest

The authors declare no conflicts of interest.

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