Chronic Toxicity Study in Rats Orally Exposed to Mulberry Sea-Buckthorn Beverage Concentrate ()
Abstract
Objective: This
study is designed to observe the chronic toxicity after the administration of
mulberry sea-buckthorn beverage concentrate for 3 months on rats and to predict
the possible adverse effect and the potential toxicity target organs. Method:
The rats (SPF level) were randomly divided into high-dose (20 mL/kg BW),
middle-dose (10 mL/kg BW), low-dose (5 mL/kg BW) groups and negative control
group (20 mL/kg BW of purified water) with 30 rats in each group. Each group
was orally given mulberry sea-buckthorn beverage concentrate for 3 months and recovered
by stop feeding samples for 2 weeks for a recovery observation. The rats’
general condition, the organ coefficient, the indexes of hematology and blood biochemistry
and the histological changes of the main organs were determined. Result: The
appearance and behavior of activity in rats showed no anomalies in all these
groups and all the rats put on weight during this period. Comparing to the negative
control group, no obvious differences were observed in the weekly weight and
organ coefficient of each dose group. After 3 months of administration, HGB in
both mulberry sea-buckthorn beverage concentrate low-dose group and high-dose
group were increased. No significant differences were observed in the indexes
of hematology after 2 weeks of recovery. CREA in low-dose, middle-dose and
high-dose groups were significantly increased after 3 months of administration
and it remained in the high level in middle-dose and high-dose group even after
2 weeks of recovery. No drug-related lesions were observed in the histological
changes of major organs. Conclusion: The results show that long term use of
mulberry concentrated sea-buckthorn beverage can lead to increased CREA, which suggested
kidney toxicity. Although no obvious pathological change was found in kidney, we
should pay attention to chronic kidney damage in the further research.
Share and Cite:
Sun, Q. and Xu, Z. (2014) Chronic Toxicity Study in Rats Orally Exposed to Mulberry Sea-Buckthorn Beverage Concentrate.
Pharmacology & Pharmacy,
5, 911-918. doi:
10.4236/pp.2014.59102.
Conflicts of Interest
The authors declare no conflicts of interest.
References
[1]
|
Efferth, T. and Kaina, B. (2011) Toxicities by Herbal Medicines with Emphasis to Traditional Chinese Medicine. Current Drug Metabolism, 12, 989-996. http://dx.doi.org/10.2174/138920011798062328
|
[2]
|
Zhou, J., Ouedraogo, M., Qu, F. and Duez, P. (2013) Potential Genotoxicity of Traditional Chinese Medicinal Plants and Phytochemicals: An Overview. Phytotherapy Research, 27, 1745-1755. http://dx.doi.org/10.1002/ptr.4942
|
[3]
|
Xia, Y.J., Tan, Z.P., Wang, L.P. and Xin, N. (2013) Research Progress of Chinese Herbal Mulberry Used as Medicine or Food. China Medicine and Pharmacy, 3, 52-54.
|
[4]
|
Chao, H.Y. (1999) Research Progress of Mulberry. Lishizhen Medicine and Materia Medica Research, 10, 6262-6281.
|
[5]
|
Ge, X.Y. (1986) Survey of Studies on the Chemical Constituents of Sea-Buckthorn. Chinese Traditional and Herbal Drugs, 17, 42.
|
[6]
|
Ruan, J.C., Huang, Y.X. and Yang, S.Y. (1995) The Acute Toxicity and Anti-Aging Test of Seabuckthorn. Chinese Journal of New Drugs and Clinical Remedies, 14, 325-327.
|
[7]
|
Lu, K.P. (1984) Effects of Sea-Buckthorn Essence for Athletic Ability. Inner Mongolia Medical Journal, 2, 8.
|
[8]
|
Chu, W. and Xu, J. (1994) Effect of Mulberry Fruit on Blood Deficiency, Blood Stasis and Immunity. Hubei Journal of Traditional Chinese Medicine, 16, 46-47.
|