Isolation and Characterization of 26 Microsatellite Loci for the Shortfin Silverside Fish Chirostoma humboldtianum Valenciennes1835 (Atherinopsidae: Menidiinae) Derived from Next Generation Sequencing and Their Cross-Amplification in Central Mexican Atherinopsids

Barriga-Sosa Irene de los Angeles, Rosa María García-Martínez, Jhoana Díaz-Larrea, Oscar Adrián Lozano Garza, Francisco Javier García-De-León

Departamento de Hidrobiología, Universidad Autónoma Metropolitana Iztapalapa, México D.F., México.
Laboratorio de Genética y Biología Molecular, Planta Experimental de Producción Acuícola, Departamento de Hidrobiología, Universidad Autónoma Metropolitana Iztapalapa, México D.F., México.
Laboratorio de Genéticapara la Conservación, Centro de Investigaciones Biológicas del Noroeste, Instituto Politécnico Nacional, La Paz, México.

DOI: 10.4236/ojgen.2014.45032                

Abstract

The endemic silverside fish C. humboldtianum is of great ichtyologycal, economical and cultural relevance in central Mexico and it has been suggested that it is among a group of other “peces blancos”, the most ancestral species. Here we characterized a set of 26 microsatellite loci from the species in order to further assess population and phylogeographic issues that aid in evaluating their highly impacted populations. From 58 primer pairs tested on specimens from Villa Victoria dam (Rio Balsas Basin), 26 loci positively amplified on the species and cross-primed on specimens of the closely related and threatened Poblana alchichica, C. promelas and C. riojai. Loci resolved medium and high genetic variation (mean N_A = 14.3, SD = 9.38; mean H_O & H_E = 0.47, SD = 0.32 and 0.58, SD = 0.32, respectively) and it is expected that these polymorphic loci are also useful in studing threatened atherinopsid species.

Keywords

Endemic, Polymorphism, Villa Victoria, Poblana alchichica, C. promelas, C. riojai

Conflicts of Interest

The authors declare no conflicts of interest.

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