A New Immunosuppressive Protein in Pig Spleen

Hongyan Fu, Juan Feng, Shaoguang Fan

Department of Physiology and Pathophysiology, Peking University Health Science Center, Beijing, China.

DOI: 10.4236/wjv.2014.43013                

Abstract

Chronic stress has been the popular phenomenon in the modern society. But we have not yet resolved the problem effectively. A novel protein in pig spleen, which possesses immunosuppressive activity, has been purified in this study. Its molecular weight about 200 kDa and three subunits composition were fractionated by SDS-PAGE. The protein inhibited mouse T-lymphocyte proliferation by the induced concanavalin A (ConA) and regulatory volume decrease (RVD), which may be due to the decreased Kv1.3 expression and activity. In addition, we have raised successfully its polyclonal antibody, which could reverse the inhibited lymphocyte proliferation by the immuno-suppressive protein. And the antibody could be used to coat plate for ELISA assay. Therefore, the antibody will have the potential to provide a basis for applying to clinical stress diseases in the future.

Keywords

Immunosuppressive Protein, Lymphocyte Proliferation, Pig, Kv1.3, RVD

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Zha, H.B., Ding, G.F. and Fan, S.G. (1992) Serum Factors Induced by Restraint Stress in Mice and Rats Suppress Lymphocyte Proliferation. Brain, Behavior, and Immunity, 6, 18-31. http://dx.doi.org/10.1016/0889-1591(92)90056-T
[2]	Shao, L., Gao, S., Zhang, Q. and Ding, G.F. (1995) A Suppressive Factor Generated in Lymph Tissue on Lymphocyte Proliferation Induced by Restraint Stress. Chinese Science Bulletin, 40, 164-166.
[3]	Fan, S.G., Gao, S., Shao, L., Mei, L. and Ding, G.F. (1995) A Suppressive Factor in Lymph Node and Spleen Induced by Restraint Stress Suppresses Lymphocyte Proliferation. Neuroimmunomodulation, 2, 274-281. http://dx.doi.org/10.1159/000097206
[4]	Fan, S.G. and Zha, H.B. (1989) Effects of Restraint Stress on the Generation of Suppressive Factors in Mouse Serum. Chinese Science Bulletin, 20, 1584-1587.
[5]	Zha, H.B., Xu, H., Zhe, Q.Z., Cheng, W. and Fan, S.G. (1991) A Study of Serum Suppressive Factors on Lymphocyte Proliferation under Restraint Stress in Rats. The Journal of Physiology, 43, 31-37.
[6]	Keller, S.E., Weiss, J.M., Schleifer, S.T., Miller, N.E. and Stein, M. (1981) Suppression of Immunity by Stress: Effect of Graded Series of Stresses on Lymphocyte Stimulation in the Rat. Science, 213, 1397-1400. http://dx.doi.org/10.1126/science.6973822
[7]	Kohler, G. and Milstein, C. (1975) Continuous Culture of Fused Cells Secreting Antibody of Predefined Specificity. Nature, 256, 495-498. http://dx.doi.org/10.1038/256495a0
[8]	Harlow, E. and Lane, D. (1975) Using Antibodies: Laboratory Manual. CSHL Press, New York, 313-346.
[9]	Wolf, J.H.N., Wu, A.V.O., Connor, N.E., Saporoschetz, I. and Manick, J.A. (1982) Immunosuppressive Serum. An Impaired Lymphocyte Blastogenesis in Burns Patients. Archives of Surgery, 117, 1266-127. http://dx.doi.org/10.1001/archsurg.1982.01380340002002
[10]	McLaughlin, G.A., Wu, A.V., Saporoschetz, I., Ninberg, R.B. and Mannick, J.A. (1979) Correlation between Anergy and a Circulating Immunosuppressive Factor Following Major Surgical Trauma. Annals of Surgery, 190, 297-304. http://dx.doi.org/10.1097/00000658-197909000-00004
[11]	Ding, G.F., Deng, H.Y., Deng, Y.L., Pang, J. and Fan, S.G. (1984) The Effect of 5, 6-Dihydroxytryptryptamine on Lymphocyte Transformation Reaction of spleen Cells in Rats. Chinese Science Bulletin, 29, 1086-1090.
[12]	Gao, S., Mei, L., Zhang, G.F. and Fan, S.G. (1984) An Inhibitory Effect of Restraint Stress on the Development of Diabetes Induced by Alloxan. Chinese Journal of Neuroimmunology and Neurology, 3, 95-98.
[13]	Fan, S.G., Shao, L. and Ding, G.F. (1996) A Suppressive Protein Generated in Peripheral Lymph Tissue Induced by Restraint Stress. Advances in Neuroimmunology, 6, 278-288. http://dx.doi.org/10.1016/S0960-5428(96)00023-X
[14]	Weber, K. and Osborn, M.J. (1969) The Reliability of Molecular Weight Determination by Dodecyl Sulphate Polyacrylamide Gel Electrophoresis. The Journal of Biological Chemistry, 244, 4406.
[15]	Walker, J.M. (1996) The Bradford for Protein Quantitation. In: Walker, J.M., Ed., The Protein Protocols Handbook, 15-20.
[16]	Alain, T. and Eric, V. (2001) Agrin—A Bridge between the Nervous and Immune Systems. Science, 292, 1667-1669. http://dx.doi.org/10.1126/science.1061542