A Comparative In-Vitro Study of Sealing Ability of Four Different Materials Used in Furcation Perforation

DOI: 10.4236/ojst.2014.48054   PDF   HTML   XML   4,115 Downloads   5,072 Views   Citations


The purpose of this study was to compare the sealing ability and marginal adaptation of four re-storative materials (MTA, Biodentine, portlant cement, and resin modified glass ionomer cement) used to repair iatrogenic furcation perforations. Eighty-four molars were treated endodontically, perforated in the middle of the pulp chamber floor with a round bur and separated randomly into 4 groups of 20 teeth each, while 4 teeth were used as positive and negative controls. The teeth were embedded in a moistened flower sponge and the perforations were filled with the appropriate restorative materials: Group 1: Biodentine; Group 2: MTA Angelus; Group 3: GC Fuji lining LC Paste Pak; Group 4: Aquafix Portland cement. The teeth remained in the soaked sponge for 28 days and then were submerged in basic fuchsine solution 1% for 48 hours. Dye penetration was evaluated after longitudinal sectioning of the teeth. Statistical analysis revealed that perforations restored with MTA exhibited the least microleakage with statistically significant difference among the other three groups (p < 0.05). The worst sealing ability was observed in the teeth restored with Aquafix Portland cement. No statistically significant difference was found between the groups of Biodentine and FC Fuji Lining Paste (p = 0.066).

Share and Cite:

Nikoloudaki, G. , Kontogiannis, T. , Meliou, H. and Kerezoudis, N. (2014) A Comparative In-Vitro Study of Sealing Ability of Four Different Materials Used in Furcation Perforation. Open Journal of Stomatology, 4, 402-411. doi: 10.4236/ojst.2014.48054.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Sinai, I. (1977) Endodontic Perforations: Their Prognosis and Treatment. Journal of the American Dental Association, 95, 90-95.
[2] Jew, R., Weine, F. and Keen, J. (1982) A Histologic Evaluation of Periodontal Tissues Adjacent to Root Perforations Filled with Cavit. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 54, 124-135.
[3] Lemon, R. (1992) Nonsurgical Repair of Perforation Defects. Dental Clinics of North America, 36, 349-457.
[4] Pitt-Ford, T.R., Torabinejad, M., Mckentry, D.J., Hong, C.U. and Kariyawasam, S.P. (1995) Use of MTA for Repair of Furcal Perforations. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 79, 756-772.
[5] Yildirim, T., Gencoglu, N., Firat, I., Perk, C. and Guzel, O. (2005) Histological Study of Furcation Perforation Treated with MTA or Super-EBA in Dog’s Teeth. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 100, 120-124.
[6] El Deeb, M.E., El Deeb, M., Tabibi, A. and Jensen, J.R. (1982) An Evaluation of the Use of Amalgam, Cavit & Calcium Hydroxide in the Repair of Furcation Perforations. Journal of Endodontics, 8, 459-466.
[7] Fischer, E.J., Arens, D.E. and Miller, C.H. (1998) Bacterial Leakage of MTA as Compared with Zinc-Free Amalgam, IRM and Super-EBA as a Root-End Filling Material. Journal of Endodontics, 24, 176-179.
[8] Alhadainy, H.A. and Hinel, V.T. (2003) Comparative Study of the Sealing Ability of Light-Cures versus Chemically Cures Materials Placed into Furcation Perforations. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 76, 338-342.
[9] Tsatsas, D., Meliou, E. and Kerezoudis, N.P. (1995) Sealing Effectiveness of Materials Used in Furcal Perforation in Vitro. International Dental Journal, 55, 133-141.
[10] Torabinejad, M., Smoth, P.W., Kettering, J.D. and Pitt Ford, T.R. (1995) Comparative Investigation of Marginal Adaptation of Mineral Trioxide Aggregate and Other Commonly Used Root-End Filling Materials. Journal of Endodontics, 21, 295-299.
[11] Sluyk, S.K., Hoon, P.C. and Hartwell, G.R. (1998) Evaluation of Setting Properties and Retention Characteristics of Mineral Trioxide Aggregate When Used as a Furcation Perforation Repair Material. Journal of Endodontics, 24, 768-771.
[12] Torabinejad, M. and Parirokh, M. (2010) Mineral Trioxide Aggregate: A Comprehensive Literature Review—Part II: Leakage and Biocompatibility Investigations. Journal of Endodontics, 36, 190-202.
[13] Torabinejad, M., Hong, C.V., McDonald, F. and Ford, T.R.P. (1995) Physical and Chemical Properties of a New Root- End Filling Material. Journal of Endodontics, 21, 349-353.
[14] Septodont, R & D Department, Biodentine, Active Biosilicate Technology, Scientific File.
[15] Zhou, H., Shen, Y., Wang, Z., Li, L., Zheng, Y.F., Häkkinen, L. and Haapasalo, M. (2013) In Vitro Cytotoxicity Evaluation of a Novel Root Repair Material. Journal of Endodontics, 39, 478-483.
[16] Aggarwal, V., Singla, M., Miglani, S. and Kohli, S. (2013) Comparative Evaluation of Push-Out Bond Strength of ProRoot MTA, Biodentine, and MTA Plus in Furcation Perforation Repair. Journal of Conservative Dentistry, 16, 462-465.
[17] Wu, M.K., Kontakiotis, E.G. and Wesselink, P.R. (1998) Decoloration of 1% Methylene Blue Solution in Contact with Dental Filling Materials. Journal of Dentistry, 26, 585-589.
[18] Meliou, H., Karoni, C., Chakmakchi, M. and Kerezoudis, N.P. (2005) Sealing Effectiveness of White ProRoot MTA. Odontostomatol Progress, 59, 372-381.
[19] Nikoloudaki, G., Meliou, H. and Kerezoudis, N.P. (2012) Comparison of Sealing Effectiveness of MTA and Biodentine in Furcation Perforation. Odontostomatol Progress, 66, 454-464.
[20] Fridland, M. and Rosato, R. (2003) Mineral Trioxide Aggregate (MTA) Solubility and Porosity with Different Water-to-Powder Ratios. Journal of Endodontics, 29, 814-817.
[21] Sarkar, N.K., Caidedo, R., Ritwi, P., Moiseyeva, R. and Kawashima, I. (2005) Physicochemical Basis of the Biologic Properties of Mineral Trioxide Aggregate. Journal of Endodontics, 21, 731-738.
[22] Camilleri, J. and Ford, T.R.P. (2006) Mineral Trioxide Aggregate: A Review of Constituents and Biological Properties of the Material. International Endodontic Journal, 39, 747-754.
[23] Al-Hazaimi, K., Al-Shalan, T.A., Naghshbandi, J., Oglesby, S., Simon, J.H. and Rotstein, I. (2006) Antibacterial Effect of Two MTA Preparations against Enterococcus faecalis and Streptococcus sanguis in Vitro. Journal of Endodontics, 32, 1053-1056.
[24] Koh, E.T., Torabinejad, M., Ford, T.R.P., Brady, K. and McDonald, F. (1997) Mineral Trioxide Aggregate Stimulates a Biological Response in Human Osteoblasts. Journal of Biomedical Materials Research, 37, 432-439.
[25] Dammaschke, T., Gerth, H.U., Zuchner, H., Brady, K. and McDonald, F. (2005) Chemical and Physical Surface and Bulk Material Characterization of White ProRoot MTA and Two Portland Cements. Dental Materials, 21, 731-738.
[26] Namazikhah, M.S., Nekoofar, M.H., Sheykhrezae, M.S., Salariyeh, S., Hayes, S.J., Bryant, S.T., Mohammadi, M.M. and Dummer, P.M. (2008) The Effect of pH on Surface Hardness and Microstructure of Mineral Trioxide Aggregate. International Endodontic Journal, 41, 108-116.
[27] Asgary, S., Parirokh, M., Eghabal, M.J. and Brink, F. (2005) Chemical Differences between White and Gray Mineral Trioxide Aggregate. Journal of Endodontics, 31, 101-103.
[28] Attin, T., Buchalla, W., Kiebassa, A.M. and Helwig, E. (1995) Curing Shrinkage and Volumetric Changes of Resin-Modified Glass Ionomer Restorative Materials. Dental Materials, 11, 359-362.
[29] Kanchanavasita, W., Antice, H.M. and Pearson, G.J. (1997) Water Absorption Characteristics of Resin-Modified Glass-Ionomer Cements. Biomaterials, 18, 343-349.
[30] Watts, D.C., Kisumbi, B.K. and Toworfe, G.K. (2000) Dimensional Changes of Resin/Ionomer Restoratives in Aqueous Neutral Media. Dental Materials, 16, 89-96.
[31] Wucherpfenning, A.L. and Green, D.B. (1999) PR 40 Mineral Trioxide vs. Portland Cement: Two Biocompatibile Filling Materials. Journal of Endodontics, 25, 308.
[32] Estrela, C., Bammann, L.L., Estrela, C.R., Silva, R.S. and Pécora, J.D. (2000) Antimicrobial and Chemical Study of MTA, Portland Cement, Calcium Hydroxide Paste, Sealapex and Dycal. Brazilian Dental Journal, 11, 3-9.
[33] De Deus, G., Ximenes, R., Gurgel-Filho, E., Plotkowski, M.C. and Coutinho-Filho, T. (2005) Cytotoxicity of MTA and Portland Cement on Human ECV 304 Endothelial Cells. International Endodontic Journal, 38, 604-609.
[34] Holland, R., De Souza, V., Nery, M.J., Faraco Jr., I.M., Bernabé, P.F., Filho, J.A.O. and Dezan Jr., E. (2001) Reaction of Rat Connective Tissue to Implanted Dentin Tube Filled with Mineral Trioxide Aggregate, Portland Cement or Calcium Hydroxide. Brazilian Dental Journal, 12, 3-8.
[35] Seux, D., Couble, M.L., Hartmann, D.J., Gauthier, J.P. and Magloire, H. (1991) Odontoblast-Like Cytodifferentiation of Human Dental Pulp Cells in Vitro in the Presence of a Calcium Hydroxide-Containing Cement. Archives of Oral Biology, 36, 117-128.
[36] Aquilina, J.W. (1999) The Physical Properties of Accelerated Portland Cement. M.Sc. Project Report, University of London, London.
[37] De Deus, G., Petruccelli, V., Gurgel-Filho, E. and Coutinho-Filho, T. (2006) MTA versus Portland Cement as Repair Material for Furcal Perforations: A Laboratory Study Using a Polymicrobial Leakage Model. International Endodontic Journal, 39, 293-298.

comments powered by Disqus

Copyright © 2020 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.