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Hypolipidemic Activity of Microwave-Dehydrated Mango (Mangifera indica L.) Powder in Mice Fed a Hypercholesterolemic Diet

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DOI: 10.4236/jbise.2014.710080    3,039 Downloads   3,657 Views   Citations

ABSTRACT

The effect of dietary supplementation with mango (Mangifera indica L.) powder obtained by microwave dehydration was studied in normal and hypercholesterolemic mice. The phenolic profiles in methanolic extracts and antioxidant activities were determined using high-performance liquid chromatography and DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2'-azino-bis-3-ethyl-benzothiazoline-6-sulphonic acid) assays, respectively. The total phenolic content of microwave-dehydrated mango powder was 1451.7 ± 26.7 μggallic acid equivalents/g dry weight. The main phenolic compounds identified were gallic acid, epicatechin, ferulic acid, and catechin. The anti-oxidant activity was 14.6% higher in microwave-dehydrated mango powder than in conventional mango powder. Mice were fed a hypercholesterolemic diet (1 g cholesterol/100g over 7 days). The hypercholesterolemic mice whose diets were supplemented with microwave-dehydrated mango powder showed a significant decrease (P ≤ 0.05) in total serum cholesterol compared to the hypercholesterolemic mice and the mice fed with conventional dehydrated mango powder. We observed a significant increase (P ≤ 0.05) in HDL-c levels in the microwave-dehydrated mango powder group mice (12.4 ± 1.3 mmol/L) compared to the hypercholesterolemic group (8.6 ± 1.4 mmol/L) and the conventional-dehydrated mango powder group (10.9 ± 1.3 mmol/L). The mice provided with microwave-dehydrated mango powder had significantly lower (P ≤ 0.05) levels of serum LDL-c (36.0%) and total cholesterol (43.3%) compared to the mice in hypercholesterolemic group. Notably, the atherogenic index was 72% lower in the microwave-dehydrated mango powder group compared to the hypercholesterolemic group. These results suggest that the biocompounds, such as polyphenols and dietary fiber, found in mango pulp might improve the lipid profile in mice fed a hypercholesterolemic diet.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Osorio-Esquivel, O. , Cortés-Viguri, V. , Garduño-Siciliano, L. , Ortiz-Moreno, A. and Sánchez-Pardo, M. (2014) Hypolipidemic Activity of Microwave-Dehydrated Mango (Mangifera indica L.) Powder in Mice Fed a Hypercholesterolemic Diet. Journal of Biomedical Science and Engineering, 7, 809-817. doi: 10.4236/jbise.2014.710080.

References

[1] U.S. Department of Agriculture (USDA), Agricultural Research Service (2009) USDA National Nutrient Database for Standard Reference, Release 22. Nutrient Data Laboratory Home Page.
http://www.ars.usda.gov/nutrientdata
[2] Hui, Y.H., Clary, C., Faid, M., Fasina, O., Noomhorn, A. and Welti-Chanes, J. (2007) Drying of Mango (Mangifera indica L.) and Mango Products. In: Food Drying Science and Technology: Microbiology, Chemistry, Applications, DEStech Publications I, Pennsylvania, 477-497.
[3] Ozkan, I.A., Akbudak, B. and Akbudak, N. (2007) Microwave Drying Characteristics of Spinach. Journal Food Engineering, 78, 577-583.
http://dx.doi.org/10.1016/j.jfoodeng.2005.10.026
[4] Matsui, N.K., Gut, W.A.J., de Oliveira, P.V. and Tadini, C.C. (2008) Inactivation Kinetics of Polyphenol Oxidase and Peroxidase in Green Coconut Water by Microwave Processing. Journal Food Engineering, 88, 169-176.
http://dx.doi.org/10.1016/j.jfoodeng.2008.02.003
[5] Hayat, K., Zhang, X., Farooq, U., Abbas, S., Xia, S., Jia, C., Zhong, F. and Zhang, J. (2010) Effect of Microwave Treatment on Phenolic Content and Antioxidant Activity of Citrus Mandarin Pomace. Food Chemistry, 123, 423-429.
http://dx.doi.org/10.1016/j.foodchem.2010.04.060
[6] Pourahmad, J., Eskandari., M.R., Shakibaei., R. and Kamalinejad, M. (2010) A Search for Hepatoprotective Activity of Fruit Extract of Mangifera indica L. against Oxidative Stress Cytotoxicity. Plant Food for Human Nutrition, 65, 83-89.
[7] Ma, X., Wu, H., Liu, L., Yao, Q., Wang, S., Zhan, R., Xing, S. and Zhou, Y. (2011) Polyphenolic Compounds and Antioxidant Properties in Mango Fruits. Scientia Horticultura-Amsterdam, 129, 102-107.
http://dx.doi.org/10.1016/j.scienta.2011.03.015
[8] Venkatesh, S.M. andRaghavan, V.S.G. (2004) An Overview of Microwave Processing and Dielectric Properties of Agri-Food Materials. Biosystems Engineering, 88, 1-18.
http://dx.doi.org/10.1016/j.biosystemseng.2004.01.007
[9] Montgomery, D. (2010) Introducción al diseño de experimentos. Diseño y análisis de experimentos. Limusa-Wiley, México, 1, 170-211.
[10] Rocha-Ribeiro, S., Queiroz, J., Lopes-Ribeiro de Queiroz, M., Campos, F. and Pinheiro Sant’Ana, H. (2007) Antioxidant in Mango (Mangifera indica L.) Pulp. Plant Food for Human Nutrition, 62, 13-17.
http://dx.doi.org/10.1007/s11130-006-0035-3
[11] Singleton, V.L., Orthofer, R. and Lamuela-Raventós, R.M. (1999) Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent. Methods in Enzymology, 299, 152-178.
http://dx.doi.org/10.1016/S0076-6879(99)99017-1
[12] Pandarinathan, S. and Sivakumar, S. (2010) Studies on Biochemical Changes in Mangoes Due to Artificial Ripening. International Journal of Agricultural Sciences, 1, 3347-3355.
[13] Brand-Williams, W., Cuvelier, M.E. and Berset, C. (1995) Use of a Free Radical Method to Evaluate Antioxidant Activity. LWT-Food Science and Technology, 28, 25-30.
http://dx.doi.org/10.1016/S0023-6438(95)80008-5
[14] AOAC (2005) Official Methods of Analysis, Vol. 1. Association of Official Analytic Chemist, Washington DC.
[15] Normas, D.G.N. (1999) Especificaciones Técnicas para la producción, cuidado y uso de los animales de laboratorio. Vol. NOM-062-ZOO-1999, Normas Mexicanas, México.
[16] Hisashi, M., Takeshi, C., Yasushi, K., Johji, Y., Tokunosuke, S., Hajime, F. and Hitoshi, K. (1986) Effects of Crude Drugs on Experimental Hypercholesterolemia. I. Tea and Its Active Principles. Journal of Ethnopharmacology, 17, 213-224.
http://dx.doi.org/10.1016/0378-8741(86)90110-8
[17] Argüelles, N., Sánchez-Sandoval, E., Mendieta, A., Villa-Tanaca, L., Garduño-Siciliano, L., Jiménez, F., Cruz, M., Medina-Franco, J.L., Chamorro-Cevallos, G. and Tamariz, J. (2010) Design, Synthesis, and Docking of Highly Hypolipidemic Agents: Schizosaccharomyces pombe as a New Model for Evaluating α-Asarone-Based HMG-CoA Reductase Inhibitors. Bioorganic & Medicinal Chemistry, 18, 4238-4248.
http://dx.doi.org/10.1016/j.bmc.2010.04.096
[18] Ajila, C.M., Naidu, K.A., Bhat, S.G. and Rao, U.J.S.P. (2007) Bioactive Compounds and Antioxidant Potential of Mango Peel Extract. Food Chemistry, 105, 982-988.
http://dx.doi.org/10.1016/j.foodchem.2007.04.052
[19] Croft, K.D. (1998) The Chemistry and Biological Effects of Flavonoids and Phenolic Acids. Annals of the New York Academy of Sciences, 854, 435-442.
[20] González-Aguilar, G.A., Villegas-Ochoa, M.A., Martínez-Téllez, M.A., Gardea, A.A. and Ayala-Zavala, J.F. (2007) Improving Antioxidant Capacity of Fresh-Cut Mangoes Treated with UV-C. Journal of Food Science, 72, S197-S202.
http://dx.doi.org/10.1111/j.1750-3841.2007.00295.x
[21] Schieber, A., Ullrich, W. and Carle, R. (2000) Characterization of Polyphenols in Mango Puree Concentrates by HPLC with Diode Array and Mass Spectrometric Detection. Innovative Food Science & Emerging Technologies, 1, 161-166. http://dx.doi.org/10.1016/S1466-8564(00)00015-1
[22] Kim, Y., Brencht, K.J. and Stephen, T.T. (2007) Antioxidant Phytochemical and Fruit Quality Changes in Mango (Mangifera indica L.) Following Hot Water Immersion and Controlled Atmosphere Storage. Food Chemistry, 105, 1327-1334.
http://dx.doi.org/10.1016/j.foodchem.2007.03.050
[23] Mahattanatawee, K., Manthey, J.A., Luzio, G., Talcott, S.T., Goodner, K. and Baldwin, E.A. (2006) Total Antioxidant Activity and Fiber Content of Select Florida-Grown Tropical Fruits. Journal of Agricultural and Food Chemistry, 54, 7355-7363.
http://dx.doi.org/10.1021/jf060566s
[24] Robles-Sánchez, M.R., Rojas-Graü, A.M., Odriozola-Serrano, I., González-Aguilar, G.A. and Martín-Belloso, O. (2009) Effect of Minimal Processing on Bioactive Compounds and Antioxidant Activity of Fresh-Cut ‘Kent’ Mango (Mangifera indica L.). Postharvest Biology and Technology, 51, 384-390.
http://dx.doi.org/10.1016/j.postharvbio.2008.09.003
[25] Liu, F.X., Fu, S.F., Bi, X.F., Chen, F., Liao, X.J., Hu, X.S. and Wu, J.H. (2013) Physico-Chemical and Antioxidant Properties of Four Mango (Mangifera indica L.) Cultivars in China. Food Chemistry, 138, 396-405.
http://dx.doi.org/10.1016/j.foodchem.2012.09.111
[26] Kuskoski, E.M., Asuero, A.G., Troncoso, A.M., Mancini-Filho, J. and Fett, R. (2005) Aplicación de diversos métodos químicos para determinar actividad antioxidante en pulpa de frutos. Ciencia y Tecnología de Alimentos, 25, 726-732. http://dx.doi.org/10.1590/S0101-20612005000400016
[27] Rocha-Ribeiro, M.S. and Schieber, A. (2010) Bioactive Compounds in Mango (Mangifera indica L.). In: Bioactive Food in Promoting Health: Fruits and Vegetables, Elsevier Publisher, Oxford, Chapter 34, 507-523.
[28] Ajila, C.M., Leelavathi, K. and Rao, U.J.S.P. (2008) Improvement of Dietary Fiber Content and Antioxidant Properties in Soft Dough Biscuits with the Incorporation of Mango Peel Powder. Journal of Cereal Science, 48, 319-326.
http://dx.doi.org/10.1016/j.jcs.2007.10.001
[29] Galisteo, M.D.J. and Zarzuelo, A. (2008) Effects of Dietary Fibers on Disturbances Clustered in the Metabolic Syndrome. The Journal of Nutritional Biochemistry, 19, 71-84.
http://dx.doi.org/10.1016/j.jnutbio.2007.02.009
[30] Prabhu, S., Mallika, J., Sabitha, K.E. and Shyamala-Devi, C.S. (2006) Effect of Mangiferin on Mitochondrial Energy Production in Experimentally Induced Myocardial Infracted Rats. Vascular Pharmacology, 44, 519-525.
http://dx.doi.org/10.1016/j.vph.2006.03.012
[31] Anila, L. and Vijayalakshmi, R.N. (2002) Flavonoids from Emblica officinalis and Mangifera indica Effectiveness for Dyslipidemia. Journal of Ethnopharmacology, 79, 81-87.
http://dx.doi.org/10.1016/S0378-8741(01)00361-0

  
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