Optical Probe for Near-Infrared (NIR) Fluorescence Signal Detection with High Optical Performance and Thermal Stability

In Hee Shin, Joo Beom Eom, Jae Seok Park, Hyeong Ju Park, Byeong-Il Lee

Bio-Photonics Research Center, Korea Photonics Technology Institute, Gwangju, Korea.

DOI: 10.4236/jbise.2014.710078                

Abstract

We propose a new optical probe for near-infrared (NIR) fluorescence signal detection with high optical performance and thermal stability. The optical probe is composed of an optical source part for efficient excitation of NIR fluorescence signal, a heat dissipation part for stable operation of the NIR fluorescence probe, and an optical detection part for efficient detection of NIR fluorescence signal. From a simulation by use of an optical simulation tool, Light Tools^TM, we could confirm that the optical probe has optical propagation efficiency of 79.6% in case of using a circular detector with 20 cm in diameter located at 20 cm in distance from the optical source. From a measurement of temperature variation of the optical probe, we could also confirm that the optical probe has thermal stability with a standard deviation of 2.19°C under room temperature condition. Finally, from an evaluation of fluorescence image quality, we could confirm that an optical noise which can bring on by overlapped band between optical spectrum of the optical source for fluorescence excitation and optical spectrum of the emitted fluorescence signal decreased effectively in the optical probe.

Keywords

Near-Infrared, Fluorescence, LEDs, Liquid Circulation Module

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Hutteman, M., Mieog, J.S.D., van der Vorst, J.R., Liefers, G.J., Putter, H., Löwik, C.W.G.M., Frangioni, J.V., van de Velde, C.J.H. and Vahrmeijer, A.L. (2011) Randomized, Double-Blind Comparison of Indocyanine Green with or without Albumin Premixing for Near-Infrared Fluorescence Imaging of Sentinel Lymph Nodes in Breast Cancer Patients. Breast Cancer Research and Treatment, 127, 163-170. http://dx.doi.org/10.1007/s10549-011-1419-0
[2]	Shin, I.H., Kim, S.K., Eom, J.B., Park, J.S., Park, H.J., Park, I.-K. and Lee, B.-I. (2013) Novel Imaging System for Positioning of the Indocyanine Green (ICG) Target; Visible Projection of the Near-Infrared Fluorescence Image. Journal of Biomedical Science and Engineering, 6, 896-900. http://dx.doi.org/10.4236/jbise.2013.69109
[3]	van der Vorst, J.R., Hutteman, M., Mieog, J.S.D., de Rooij, K.E., Kaijzel, E.L., Löwik, C.W.G.M., Putter, H., Kuppen, P.J.K., van de Velde, C.J. and Vahrmeijer, A.L. (2012) Near-Infrared Fluorescence Imaging of Liver Metastases in Rats Using Indocyanine Green. Journal of Surgery Research, 174, 266-271. http://dx.doi.org/10.1016/j.jss.2011.01.009
[4]	Miego, J.S.D., Hutteman, M., van der Vorst, J.R., Kuppen, P.J.K., Que, I., Dijkstra, J., Kaijzel, E.L., Prins, F., Lowik, C.W.G.M., Smit, V.T.H.B.M., van de Velde, C.J.H. and Vahrmeijer, A.L. (2011) Image-Guided Tumor Resection Using Real-Time Near-Infrared Fluorescence in a Syngeneic Rat Model of Primary Breast Cancer. Breast Cancer Research and Treatment, 128, 279-689. http://dx.doi.org/10.1007/s10549-010-1130-6
[5]	Troyan, S.L., Kianzad, V., Gibbs-Strauss, S.L., Gioux, S., Matsui, A., Oketokoun, R., Ngo, L., Khamene, A., Azar, F. and Frangioni, J.V. (2009) The FLARE Intraoperative Near-Infrared Fluorescence Imaging System: A First-in-Human Clinical Trial in Breast Cancer Sentinel Lymph Node Mapping. Annals of Surgical Oncology, 16, 2943-2952. http://dx.doi.org/10.1245/s10434-009-0594-2
[6]	Murawa, D., Hirche, C., Dresel, S. and Hunerbein, M. (2009) Sentinel Lymph Node Biopsy in Breast Cancer Guided by Indocyanine Green Fluorescence. British Journal of Surgery, 96, 1289-1294. http://dx.doi.org/10.1002/bjs.6721
[7]	Sevick-Muraca, E.M., Sharma, R., Rasmussen, J.C., Marshall, M.V., Wendt, J.A., Pham, H.Q., Bonefas, E., Houston, J. P., Sampath, L., Adams, K.E., Blanchard, D.K., Fisher, R.E., Chiang, S.B., Elledge, R. and Mawad, M.E. (2008) Imaging of Lymph Flow in Breast Cancer Patients after Microdose Administration of a Near-Infrared Fluoro-Phore: Feasibility Study. Radiology, 246, 734-741. http://dx.doi.org/10.1148/radiol.2463070962
[8]	Tagaya, N., Yamazaki, R. and Nakagawa, A. (2008) In-traoperative Identification of Sentinel Lymph Nodes by Near-Infrared Fluorescence Imaging in Patients with Breast Cancer. American Journal of Surgery, 195, 850-853. http://dx.doi.org/10.1016/j.amjsurg.2007.02.032
[9]	Gioux, S., Kianzad, V., Ciocan, R., Gupta, S., Oketokoun, R. and Frangioni, J.V. (2009) High Power, Computer-Controlled, LED-Based Light Sources for Fluorescence Imaging and Image-Guided Surgery. Molecular Imaging, 3, 156-165.
[10]	Gioux, S., Choi, H.S. and Frangioni, J.V. (2010) Image-Guided Surgery Using Invisible Near-Infrared Light: Fundamentals of Clinical Translation. Molecular Imaging, 9, 237-255.
[11]	Miwa, M. (2008) The Principle of ICG Fluorescence Method. The Open Surgical Oncology Journal, 2, 26-28. http://dx.doi.org/10.2174/1876504101002020026