Wastewater Remediation Using Algae Grown on a Substrate for Biomass and Biofuel Production

James B. Houser, Mark E. Venable, Yosuke Sakamachi, Michael S. Hambourger, Jacob Herrin, Shea R. Tuberty

Department of Biology, Appalachian State University, Boone, USA.
Department of Chemistry, Appalachian State University, Boone, USA.
Department of Technology and Environmental Design, Appalachian State University, Boone, USA.

DOI: 10.4236/jep.2014.510091                

Abstract

Surging oil, feed and fertilizer costs have impacted farmers particularly hard. Farm-based, local sources of renewable energy could help reduce energy costs for farmers and help develop rural-based processing and manufacturing of biofuel to bolster rural economies. At the same time, nutrient contamination and eutrophication from farming operations have become national problems. Algal-based bioprocessors have the potential to address these problems simultaneously. At Appalachian State University (Appstate) we set out to design, build and test a system that uses algae to capture wastewater nutrients as well as excreted pharmaceuticals, while simultaneously sequestering CO₂, producing oil for conversion to biodiesel and feed for livestock. There are a number of problems with current algae growth systems. Algae grown in an open pond or raceway system are suspended in the water in the presence of soluble and suspended waste making most of the current harvest techniques problematic and expensive. Appstate designed algae troughs in which the algae are immobilized on a solid substrate. The laboratory-scale prototype was constructed of three-sided square plastic pipe open at the top. Inside the pipe, there was a series of cloth filters supported by rigid flow-through baffles. Preliminary results observed an average percent reduction of nitrate and phosphorous of 40 and 43, respectively, from different initial nutrient concentrations. Near complete removal (~96%) of estrogen was observed in 2-day trial experiments. In addition, effective increases in algal biomass which can serve as both biofuel feedstock and livestock feed were observed.

Keywords

Algae, Wastewater Remediation, CAFO, Eutrophication, Biomass Production, Biofuels

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Boyd, C.E. (1990) Water Quality in Ponds for Aquaculture. Birmingham Publishing Co., Alabama.
[2]	Codd, G.A. (1995) Cyanobacterial Toxins: Occurrence, Properties and Biological Significance. Water Science & Technology, 32, 149-156. http://dx.doi.org/10.1016/0273-1223(95)00692-3
[3]	Lawton, L.A. and Codd, G.A. (1991) Cyanobacterial (Blue-Green Algal) Toxins and Their Significance in UK and European Waters. Journal of the Institution of Water and Environmental Management, 5, 460-465. http://dx.doi.org/10.1111/j.1747-6593.1991.tb00643.x
[4]	Izaguirre, G., Hwant, C.J., Krasner, S.W. and McGuire, M.J. (1982) Geosmin and 2-Methlyisoboreol from Cyanobacteria in Three Water Supply Systems. Applied and Environmental Microbiology, 43, 708-714.
[5]	Walker Jr., W.W. (1983) Significance of Eutrophication in Water Supply Reservoirs. Journal of the American Water Works Association, 75, 38-42.
[6]	EPA, United States Environmental Protection Agency (2000) The Quality of Our Nation’s Waters: A Summary of the National Water Quality Inventory: 1998 Report to Congress. USEPA, Washington DC.
[7]	Puckett, L.J. (1995) Identifying the Major Sources of Nutrient Water Pollution. Environmental Science and Technology, 29, 408-414. http://dx.doi.org/10.1021/es00009a743
[8]	Daniel, T.C., Edwards, D.R. and Sharpley, A.N. (1993) Effect of Extractable Soil Surface P on Runoff Water Quality. Transactions of the American Society of Agriculture Engineers, 36, 1079-1085. http://dx.doi.org/10.13031/2013.28437
[9]	Gilliam, J.W. (1995) Phosphorus Control Strategies. Ecological Engineering, 5, 405-414. http://dx.doi.org/10.1016/0925-8574(95)00035-6
[10]	McFarland, A.M.S. and Hauck, L.M. (1999) Relating Agricultural Land Uses to In-Stream Stormwater Quality. Journal of Environmental Quality, 28, 836-844. http://dx.doi.org/10.2134/jeq1999.00472425002800030014x
[11]	Reddy, K.R., Khaleel, R., Overcash, M.R. and Westerman, P.W. (1979) Phosphorus—A Potential Nonpoint Source Pollution Problem in Land Areas Receiving Long-Term Application of Wastes. In: Loehr, R.C., Haith, D.A., Walter, M.F. and Martin, C.S., Eds., Best Management Practices for Agriculture and Silviculture, Ann Arbor Science Publishers Inc., Ann Arbor, 193-212.
[12]	Sharpley, A.N., Daniel, T.C. and Edwards, D.R. (1993) Phosphorus Movement in the Landscape. Journal of Production Agriculture, 6, 492-500. http://dx.doi.org/10.2134/jpa1993.0492
[13]	Finley, R.L., Collignon, P., Larsson, D.G.J., McEwen, S.A., Li, X.Z., Gaze, W.H., Reid-Smith, R., Timinouni, M., Graham, D.W. and Topp, E. (2013) The Scourge of Antibiotic Resistance: The Important Role of the Environment. Clinical Infectious Diseases, 57, 704-710. http://dx.doi.org/10.1093/cid/cit355
[14]	Pruden, A., Larsson, D.G.J., Amézquita, A., Collignon, P., Brandt, K.K., Graham, D.W., Lazorchak, J.M., Suzuki, S., Silley, P., Snape, J.R., Topp, E., Zhang, T. and Zhu, Y.G. (2013) Management Options for Reducing the Release of Antibiotics and Antibiotic Resistance Genes to the Environment. Environmental Health Perspectives, 121, 878-885. http://dx.doi.org/10.1289/ehp.1206446
[15]	Bringolf, R.B., Heltsley, R.M., Newton, T.J., Eads, C.B., Fraley, S.J., Shea, D. and Cope, W.G. (2010) Environmental Occurrence and Reproductive Effects of the Pharmaceutical Fluoxetine in Native Freshwater Mussels. Environmental Toxicology and Chemistry, 29, 1311-1318. http://dx.doi.org/10.1002/etc.157
[16]	Hazelton, P.D., Cope, W.G., Mosher, S., Pandolfo, T.J., Belden, J.B., Barnhart, M.C. and Bringolf, R.B. (2013) Fluoxetine Alters Adult Freshwater Mussel Behavior and Larval Metamorphosis. Science of the Total Environment, 445-446, 94-100. http://dx.doi.org/10.1016/j.scitotenv.2012.12.026
[17]	Fong, P.P. and Molnar, N. (2008) Norfluoxetine Induces Spawning and Parturition in Estuarine and Freshwater Bivalves. Bulletin of Environmental Contamination and Toxicology, 81, 535-538. http://dx.doi.org/10.1007/s00128-008-9558-7
[18]	Painter, M.M., Buerkley, M.A., Julius, M.L., Vajda, A.M., Norris, D.O., Barber, L.B., Furlong, E.T., Schultz, I.M.M. and Schoenfuss, H.L. (2009) Antidepressants at Environmentally Relevant Concentrations Affect Predator Avoidance Behavior of Larval Fathead Minnows (Pimephales promelas). Environmental Toxicology and Chemistry, 28, 2677-2684. http://dx.doi.org/10.1897/08-556.1
[19]	Gaworecki, K.M. and Klaine, S.J. (2008) Behavioral and Biochemical Responses of Hybrid Striped Bass during and after Fluoxetine Exposure. Aquatic Toxicology, 88, 207-213. http://dx.doi.org/10.1016/j.aquatox.2008.04.011
[20]	Hinck, J.E., Blazer, V.S., Schmitt, C.J., Papoulias, D.M. and Tillitt, D.E. (2009) Widespread Occurrence of Intersex in Black Basses (Micropterus spp.) from US Rivers, 1995-2004. Aquatic Toxicology, 95, 60-70. http://dx.doi.org/10.1016/j.aquatox.2009.08.001
[21]	Silva de Assis, H.C., Simmons, D.B.D., Zamora, J.M., Lado, W.E., Al-Ansari, A.M., Sherry, J.P., Blais, J.M., Metcalfe, C.D. and Trudeau, V.L. (2013) Estrogen-Like Effects in Male Goldfish Co-Exposed to Fluoxetine and 17 Alpha-Ethinylestradiol. Environmental Science & Technology, 47, 5372-5382. http://dx.doi.org/10.1021/es3044888
[22]	Gust, D., Kramer, D., Moore, A., Moore, T.A. and Vermaas, W. (2008) Engineered and Artificial Photosynthesis: Human Ingenuity Enters the Game. MRS Bulletin, 33, 383-387. http://dx.doi.org/10.1557/mrs2008.78
[23]	Della Greca, M., Pinto, G., Pistillo, P., Pollio, A., Previtera, L. and Temussi, F. (2008) Biotransformation of Ethinylestradiol by Microalgae. Chemosphere, 70, 2047-2053. http://dx.doi.org/10.1016/j.chemosphere.2007.09.011
[24]	Molina Grima, E., Belarbi, E.H., Acién Fernández, F.G., Robles Medina, A. and Chisti, Y. (2003) Recovery of Microalgal Biomass and Metabolites: Process Options and Economics. Biotechnology Advances, 20, 491-515. http://dx.doi.org/10.1016/S0734-9750(02)00050-2
[25]	Adey, W.H., Kangas, P.C. and Mulbry, W. (2011) Algal Turf Scrubbing: Cleaning Surface Waters with Solar Energy while Producing a Biofuel. Bioscience, 61, 434-441. http://dx.doi.org/10.1525/bio.2011.61.6.5
[26]	Growing ALGAE. Algae Media. http://www.growing-algae.com/algae-media.html
[27]	Song, H.L., Yang, X.L., Nakano, K., Nomura, M., Nishimura, O. and Li, X.N. (2011) Elimination of Estrogens and Estrogenic Activity from Sewage Treatment Works Effluents in Subsurface and Surface Flow Constructed Wetlands. International Journal of Environmental Analytical Chemistry, 91, 600-614. http://dx.doi.org/10.1080/03067319.2010.496046
[28]	Sychrová, E., Stěpánková, T., Nováková, K., Bláha, L., Giesy, J.P. and Hilscherová, K. (2012) Estrogenic Activity in Extracts and Exudates of Cyanobacteria and Green Algae. Environment International, 39, 134-140. http://dx.doi.org/10.1016/j.envint.2011.10.004
[29]	Demirbas, A. and Demirbas, M.F. (2011) Importance of Algae Oil as a Source of Biodiesel. Energy Conversion and Management, 52, 163-170. http://dx.doi.org/10.1016/j.enconman.2010.06.055
[30]	Drapcho, C., Nghiem, J. and Walker, T. (2008) Biofuels Engineering Process Technology. McGraw-Hill Co., New York.
[31]	Biofuels Connect (2013) Daily B100 Biodiesel Prices. http://www.biofuelsconnect.com/
[32]	Brennan, L. and Owende, P. (2010) Biofuels from Microalgae—A Review of Technologies for Production, Processing, and Extractions of Biofuels and Co-Products. Renewable and Sustainable Energy Reviews, 14, 557-577.