Enhancement of Periodontal Healing by Application of a Novel Ointment Compared with Hyaluronic Acid, Histological Observation in Animal Model

Ghanbari Habiboallah, Zakeri Mahdi, Saghravanian Nasroallah, Zakeri Massoud, Baradaran Nasseri Ehsan, Zareian Jahromi Mina, Sardari Kamran

Department of Oral and Maxillofacial Pathology, School of Dentistry and Maxillofacial Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Periodontics, School of Dentistry and Dental Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
School of Dentistry, Mashhad University of Medical Science, Mashhad, Iran.
School of Dentistry, Shahed University, Tehran, Iran.
School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.

DOI: 10.4236/mri.2014.33009                

Abstract

Objectives: The early healing of the periodontal wound in oral cavity may be critical to the treatment outcome. Thus, we made a novel ointment based on natural constituents that our recent investigation suggested a positive therapeutic effects on gingival wounds. The present study was conducted to evaluate the role of this novel biomaterial on periodontal wound healing including soft as well as hard tissue. Materials and Methods: Experimental periodontitis was induced by wire tied around the cement-enamel junction of I₂, P₁, P₃ and M₁ in six adult, healthy beagle dogs. After 3 months, mucoperiostal flap elevated and root surfaced was thoroughly scaled and planed. Randomly, three materials including our ointment with two different ratios including materials A and B and hyluronic acid were applied topically and then the flaps were repositioned and sutured. Three dogs at day 7 and the three remaining ones two weeks post-surgery were sacrificed and histological changes were monitored. Results: The result of this study showed that the local applications of this novel ointment promoted the healing process particularly fibroblast proliferation and collagen density remarkably in comparison with hyaluronic acid and control group. Besides, histological finding of our material revealed that in general terms had all the hallmarks of complete healing two weeks post operatively. Conclusion: Our novel material appeared to have outstanding effect on periodontal wound healing which can improve the periodontal healing consequences and provide better strength of periodontal wound flap. From a clinical standpoint, it could play an important role for better repair in diabetic patients who frequently present difficulties in mucosal and cutanous wound healing.

Keywords

Anti-Inflammatory, Healing, Hyaluronic Acid, Ghee, Periodontal Flap Surgery, Animal Model

Conflicts of Interest

The authors declare no conflicts of interest.

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