Age and Gender Differences in Cytokine Profile of Lymph and Blood Serum

Alexandr Poveshchenko, Nikolai Orlov, Oleg Kazakov, Olga Poveshchenko, Irina Kim, Natali Bondarenko, Tatjana Miller, Dmitri Strunkin, Alexei Kabakov, Tatjana Reiter, Vladimir Konenkov

FSBI Scientific Institution of Clinical and Experimental Lymphology SB RAMS, Novosibirsk, Russia.

DOI: 10.4236/aar.2014.33030                

Abstract

We studied the cytokine profile (IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, GM-CSF, interferon-γ, TNFα,) of the lymph, blood serum and conditioned medium of the lymph cells of Wistar rats. It was found that the concentration of cytokines investigated in the lymph of Wistar rats was significantly higher compared to the serum. Age and gender differences between the concentration of pro- and anti-inflammatory cytokines in the lymph and blood serum were found. The concentration of the cytokines was determined using the test system “Bio-Rad”.

Keywords

Cytokines, Lymph, Blood Serum

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Poveshchenko, O., Kolesnikov, A., Kim, I., Ulyanov, E., Mozzherina, A., Jankajte, E., Solovieva, A., Herter, T., Poveshchenko, A. and Konenkov, V. ( 2008 ) Methods for Isolating and Culturing Conditions Mesenchymal Stromal Cells from Human Adipose Tissue Obtained from Various Sources. Bulletin of RAMS, 133, 90-95.
[2]	Poveshchenko, A., Abramov, V. and Kozlov, V. (2007) Cytokines—Factors Neuroendocrine Regulation. Advances of Physiological Sciences, 3, 40-46.
[3]	Kuznetsov, A. (1993) A New Way of Sampling Lymph Animals. Bulletin of Experimental Biology and Medicin, 116, 329-331.
[4]	Xin, D., Harris, M., Wade, K., Amin, M., Barr, A. and Barbe, M. (2011) Aging Enhances Serum Cytokine Response but Not Task-Induced Grip Strength Declines in a Rat Model of Work-Related Musculoskeletal Disorders. BMC Musculoskeletal Disorders, 12, 63. http://dx.doi.org/10.1186/1471-2474-12-63
[5]	Forsey, R., Thompson, J., Ernerudh, J., et al. (2003) Plasma Cytokine Profiles in Elderly Humans. Mechanisms of Ageing and Development, 124, 487-493. http://dx.doi.org/10.1016/S0047-6374(03)00025-3
[6]	Cecchini, G., Dominguez, M., Mocci, S., Wetterwald, A., Felix, R., Fleisch, H., Chisholm, O., Hofstetter, W., Pollard, J. and Stanley, R. (1994) Role of Colony Stimulating Factor-1 in the Establishment and Regulation of Tissue Macrophages during Postnatal Development of the Mouse. Development, 120, 1357-1372.
[7]	Kozlov, V., Poveshchenko, A. and Gromykhina, N. (1990) Some Mechanisms Involved in the Prostaglandin E2 Immunosuppressive Effect in (CBA X C57BL) F1 Mice in Vivo. Cellular Immunology, 128, 242-249. http://dx.doi.org/10.1016/0008-8749(90)90022-J
[8]	Rink, L., Cakman, I. and Kirchner H. (1998) Altered Cytokine Production in the Elderly. Mechanisms of Ageing and Development, 102, 199-209. http://dx.doi.org/10.1016/S0047-6374(97)00153-X