Determination of Candesartan and Hydrochlorothiazide in Human Plasma by HPLC Coupled with Mass Spectrometry

Olga S. Brushinina, Roman V. Gurto, Maksim S. Timofeev, Galina A. Frelikh, Vladimir A. Slepichev, Elena A. Yanovskaya, Natalia U. Polomeeva, Yulya G. Zyuz’kova, Vladimir V. Udut

Federal Governmental Budgetary Institution “Research Institute of Pharmacology E.D. Goldberg’s Name” Siberian Branch of the Russian Academy of Medical Sciences, Tomsk, Russia.
Federal Governmental Budgetary Institution “Research Institute of Pharmacology E.D. Goldberg’s Name”Siberian Branch of the Russian Academy of Medical Sciences, Tomsk, Russia.

DOI: 10.4236/ijamsc.2014.22003                

Abstract

Quantitative determination of hydrochlorothiazide (HCTZ) and candesartan (CDS) in human plasma in volunteers was performed using a sensitive, selective and specific LC-MS method which has been developed and validated before the study. The study was performed by means of a liquid chromatograph Shimadzu Prominence equipped with a mass spectrometer LCMS-2020. Analytical column PerfectBond ODS-HD HPLC-column 5 μm 250 × 3.0 mm with a pre-column cartridge PerfectBond ODS-HD 5 μm 10 × 3.0 mm, double source of ionization for LCMS-2020 (electrospray (ESI) and chemical (APCI)) and software LabSol LCMS V5 LCMS2020 systempack were used. The low limit of the quantitative determination for HCTZ and CDS made up 10 ng/ml. m/z for CDS 441.20—positive scan, m/z for HCTZ 295.90—negative scan. The method has been applied to a pharmacokinetic study of 12.5 mg HCTZ and 16 mg CDS tablet in healthy volunteers.

Keywords

Candesartan, Hydrochlorothiazide, LC-MS, Pharmacokinetic

Conflicts of Interest

The authors declare no conflicts of interest.

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