Methods for Breaking Dormancy of Seeds of Tropical Forage Legumes

Leonardo F. de Morais, João C. C. Almeida, Bruno B. Deminicis, Fábio T. de Pádua, Mirton J. F. Morenz, João B. R. de Abreu, Raphael P. Araujo, Delci D. de Nepomuceno

Brazilian Agricultural Research Corporation, National Research Center of Dairy Cattle, Juiz de Fora, Brazil.
Department of Animal Science and Production, Federal Rural University of Rio de Janeiro, Rio de Janeiro, Brazil.
Department of Animal Science and Production, Federal Rural University of Rio de Janeiro,Rio de Janeiro, Brazil.
Department of Animal Science and Production, Federal University of Espírito Santo,Vitória, Brazil.
Department of Animal Science, Federal University of S?o Jo?o Del Rei, S?o Jo?o Del Rei, Brazil.
Federal Institute of Rio de Janeiro-IFRJ, Campus Nilo Pe?anha-Pinheiral-RJ, Pinheiral, Brazil.

DOI: 10.4236/ajps.2014.513196                

Abstract

The aim of this study was to evaluate methods for breaking dormancy of seeds Neonotonia wightii (perennial soybean), Macrotiloma axilare (archer), Pueraria phaseoloides (tropical kudzu), Calopogonium mucunoides (calopo), which were subjected to the following treatments for physical breaks and physiological dormancy: 1) control; 2) scarification with sandpaper; 3) immersion in H₂SO₄ 98% for five minutes and subsequent washing in water; 4) preheating at 60°C for 150 minutes in an air circulating oven; 5) 0.2% KNO₃; and 6) gibberellic acid (GA₃ 0.5%). The results showed that using H₂SO₄ to break seed dormancy archer and perennial soybean and calopo scarification with sandpaper were the most recommended treatments. Tropical Kudzu presented physiological response to treatments with the use of gibberellic acid and physical treatment using immersion H₂SO₄. Thus, it is necessary to use techniques to make the breaking dormancy of seeds of legumes, resulting in an increase in the rate of seed germination and rapid deployment of the legume.

Keywords

Fabaceae, Seed, Dormancy and Germination

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Souza, S.O., Santana, J. and Shimoda, A. (2002) Behavior of Isolated Tropical Forage Grasses and Legumes in Association with the North Fluminense. Science and Agrotechnology, 26, 1554-1561.
[2]	Kyneur, G.W. (1962) The Role of Fertiliseres in Establishing Glycine javanica. L. on Latosol Soil. Proceedings. N. Queensl. Agros. Conf., 10-15.
[3]	Fernandez, C.D., Grof, B. and Carvalho, J. (2000) Mechanical Scarification of Seeds of Stylosanthes spp. with Rice Processing Equipment. Embrapa, 4 p.
[4]	Perez, S.C.J.G.A. (2004) Wraps. In: Ferreira, A.G. and Borghetti, F., Eds., Germination: From Basic to Applied, Artmed, Porto Alegre, 125-134.
[5]	McDonald, M.B. (1998) Seed Quality Assessment. Seed Science Research, Wallingford, 8, 265-275.
[6]	Sune, A.D. and Franke, L.B. (2006) Dormancy Breaking and Germination Testing Methodologies in Trifolium riograndense Burkart and Desmanthus depressus Humb. Brazilian Journal of Seeds, 28, 29-36.
[7]	Deminicis, B.B., Almeida, J.C.C., Blume, M.C., Araújo, S.A.C., Pádua, F.T., Zanine, A.M. and Jaccoud, C.F. (2006) Overcoming Dormancy in Seeds of Eight Tropical Forage Legumes. Archivos de Zootecnia, 55, 401-404.
[8]	Adams, R.M., Koenigsberg, S.S. and Langhans, R.W. (1979) In Vitro Propagation of Cephallotus folicularis (Australian Patcher Plant). Horticultural Science, 14, 512-513.
[9]	Brasil (2009) Ministério da Agricultura, Pecuária e Abastecimento. Rules for Testing Seeds. Brasilia: SNDA/DNDV/CLAV, 395 p.
[10]	Neme, A.N. (1963) Perennial Soybean Seeds. Results of Scarification and Duration of Power Twinning. Bragantia: Scientific Bulletin of the Agricultural Institute of the Sao Paulo State, 22, 785-791.
[11]	Silva, K.B., Mata, M.F. and Bruno, R.L.A. (2012) Pregerminative Treatments to Overcome Dormancy of Sterculia striata A. St. Hil. & Naldin Seeds. Semina: Ciências Agrárias, 33, 857-866. http://dx.doi.org/10.5433/1679-0359.2012v33n3p857
[12]	Santarém, E.R. and Aquila, M.E.A. (1995) Influence of Methods of Scarification and Storage on Seed Germination of Senna macranthera (Colladon) Irwin & Barneby (Leguminosae). Brazilian Journal of Seeds, 17, 205-209.
[13]	Bilia, D.A.C., Barbedo, C.J. and Maluf, A.M. (1998) Germination of Diaspores of Ocotea corymbosa (Meissn.) Mez—(Lauraceae) as a Function of Temperature, Substrate and Numbness. Brazilian Journal of Seeds, 20, 189-194.
[14]	Bertalot, M.J.A. and Nakagawa, J. (1998) Overcoming Dormancy in Seeds of Leucaena diversifolia (Schlecht.) Bentham K156. Brazilian Journal of Seeds, 20, 39-42.
[15]	Medeiros Filho, S., França, E.A. and Innecco, R. (2002) Germination Seeds of Operculina macrocarpa (L.) Farwel e Operculina alata (Ham.) Urban. Brazilian Journal of Seeds, 24, 102-107.
[16]	Roversi, T., Mattei, V.L., Silveira Júnior, P. and Falck, G.L. (2002) Overcoming Dormancy in Seeds of Acacia mearnsii Willd. Brazilian Journal of Agroscience, 8, 161-163.
[17]	Lopes, J.C., Dias, P.C. and Macedo, C.M.P. (2006) Treatments to Accelerate Germination and Reduce the Deterioration of Seeds Ormosia nitida Vog. Tree Magazine, 30, 171-177.
[18]	Cruz, E.D. and Carvalho, J.E.U. (2006) Methods of Overcoming Dormancy in Schizolobium amazonicum Huber ex Ducke (Leguminosae—Caesalpinioideae) Seeds. Brazilian Journal of Seeds, 28, 108-115.
[19]	Dutra, A.S., Medeiros Filho, S., Teófilo, E.M. and Diniz, F.O. (2007) Seeds Germination of Senna siamea (Lam.) H.S. Irwin e Barneby—Caesalpinoideae. Brazilian Journal of Seeds, 29, 160-164.
[20]	Burin, M.E. (1979) Chemical Regulation of Endogenous Dormancy of Stylosanthes humilis H.B.K. Master’s Dissertation, UFV, Viçosa, 51 p.
[21]	Deminicis, B.B. (2009) Potential Physiological Seed for Deployment by Cattle Pastures. Uenf, Thesis (Doctor of Plant Production), Darcy Ribeiro State University of Northern of Rio de Janeiro, Campos dos Goytacazes, 143 p.
[22]	Fowler, J.A.P. and Bianchetti, A. (2000) Dormancy in Tree Seeds. Embrapa Florestais, Colombo (Document, n. 40).
[23]	Coelho, M.F.B., Maia, S.S.S., Oliveira, A.K. and Diógenes, F.E.P. (2010) Overcoming Seed Dormancy in Cutaneous Caesalpinia ferrea Mart ex Tul. Brazilian Journal of Agricultural Sciences, 5, 74-79.
[24]	Benedito, C.P., Torres, S.B., Ribeiro, M.C.C. and Nunes, T.A. (2008) Overcoming Seed Dormancy in Piptadenia moniliformis Benth. Agronomic Science Magazine, 39, 90-93.
[25]	Azeredo, G.A., Paula, R.C., Valeri, S.V. and Moro, F.V. (2010) Overcoming Seed Dormancy in Piptadenia moniliformis Benth. Brazilian Journal of Seeds, 32, 49-58.
[26]	Garcia, J., Duarte, J.B. and Frasseto, E.G. (2002) Overcoming Seed Dormancy in Mimosa caesalpiniaefolia L. Tropical Agricultural Research, 32, 29-31.
[27]	Farias, R.M., Freitas, R.M.O., Nogueira, N.W. and Dombroski, J.L.D. (2013) Overcoming of Dormancy of Piptadenia stipulacea Seeds. Amazonian Journal of Agricultural and Environment Sciences, 56, 160-165.
[28]	Eschiapati-Ferreira, M.S. and Perez, S.C.J.G.A. (1997) Treatments to Overcome Dormancy Seeds of Senna macranthera (Collad.) Irwin et Barn. (Fabaceae-Caesalpinoideae). Brazilian Journal of Seeds, 19, 230-236.
[29]	Bianchetti, A., Teixeira, C.A.D. and Martins, E.P. (1998) Acid Scarification to Overcome Dormancy Seeds of Parkia multijuga Benth. Brazilian Journal of Seeds, 20, 215-218.
[30]	Jeller, H. and Perez, S.C.J.G.A. (1999) Estudo da superação da dormência e da temperatura em sementes de Cassia excelsa Schrad. Brazilian Journal of Seeds, 21, 32-40.
[31]	Alves, M.C.S., Medeiros Filho, S., Andrade Neto, M. and Teófilo, E.M. (2000) Overcoming Seed Dormancy in Bauhinia monandra britt. and Bauhinia ungulata L.-Cesalpinoideae. Brazilian Journal of Seeds, 22, 139-144.
[32]	Barbosa, A.P., Sampaio, P.T.B., Campos, M.A.A., Varela, V.P., Gonçalves, C.Q.B. and Iida, S. (2004) Alternative Technology for Breaking Seed Dormancy of Ochroma lagopus Sw, Bombacaceae. Acta Amazônica, 34, 107-110. http://dx.doi.org/10.1590/S0044-59672004000100013
[33]	Alves, E.U., Bruno, R.L.A., Oliveira, A.P., Alves, A.U. and Alves, A.U. (2006) Sulfuric Acid in Breaking Dormancy of Dispersal Units of Zizyphus joazeiro Mart. Tree Magazine, 30, 187-195.