Cell Proliferation Ability of Mouse Fibroblast-Like Cells and Osteoblast-Like Cells on a Ti-6Al-4V Alloy Film Produced by Selective Laser Melting
Mayu Kawase, Tatsuhide Hayashi, Masaki Asakura, Akimichi Mieki, Hironari Fuyamada, Masahiro Sassa, Shizuka Nakano, Masashi Hagiwara, Toru Shimizu, Tatsushi Kawai
Advanced Laser and Process Technology Research Association (ALPROT), Tokyo, Japan; Laser Sintering Department, ASPECT Inc., Tokyo, Japan.
Advanced Laser and Process Technology Research Association (ALPROT), Tokyo, Japan; Low-Formability-Materials Processing Group, Advanced Manufacturing Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan.
Department of Dental Materials Science, Aichi Gakuin University School of Dentistry, Nagoya, Japan.
Department of Dental Materials Science, Aichi Gakuin University School of Dentistry, Nagoya, Japan; Advanced Laser and Process Technology Research Association (ALPROT), Tokyo, Japan.
Department of Gerodontology, Aichi Gakuin University School of Dentistry, Nagoya, Japan.
DOI: 10.4236/msa.2014.57051   PDF   HTML     3,247 Downloads   4,596 Views   Citations

Abstract

Successful regeneration of tissues and organs relies on the application of suitable substrates or scaffolds in scaffold-based regenerative medicine. In this study, Ti-6Al-4V alloy films (Ti alloy film) were produced using a three-dimensional printing technique called Selective Laser Melting (SLM), which is one of the metal additive manufacturing techniques. The thickness of produced Ti alloy film was approximately 250 μm. The laser-irradiated surface of Ti alloy film had a relatively smooth yet porous surface. The non-irradiated surface was also porous but also retained a lot of partially melted Ti-6Al-4V powder. Cell proliferation ability of mouse fibroblast-like cells (L929 cells) and mouse osteoblast-like cells (MC3T3-E1 cells) on both the surfaces of Ti alloy film was examined using WST assay. Both L929 and MC3T3-E1 cells underwent cell proliferation during the culture period. These results indicate that selective laser melting is suitable for producing a cell-compatible Ti-6Al-4V alloy film for biomaterials applications.

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Kawase, M. , Hayashi, T. , Asakura, M. , Mieki, A. , Fuyamada, H. , Sassa, M. , Nakano, S. , Hagiwara, M. , Shimizu, T. and Kawai, T. (2014) Cell Proliferation Ability of Mouse Fibroblast-Like Cells and Osteoblast-Like Cells on a Ti-6Al-4V Alloy Film Produced by Selective Laser Melting. Materials Sciences and Applications, 5, 475-483. doi: 10.4236/msa.2014.57051.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Tabata, Y. (2004) Tissue Regeneration Based on Tissue Engineering Technology. Congenital Anomalies (Kyoto), 44, 111-124.
http://dx.doi.org/10.1111/j.1741-4520.2004.00024.x
[2] Langer, R. and Vacanti, J.P. (1993) Tissue Engineering. Science, 260, 920-926.
http://dx.doi.org/10.1126/science.8493529
[3] Warnke, P.H., Douglas, T., Wollny, P., Sherry, E., Steiner, M., Galonska, S., Becker, S.T., Springer, I.N., Witfang, J.W. and Sivananthan, S. (2009) Rapid Prototyping: Porous Titanium Alloy Scaffolds Produced by Selective Laser Melting for Bone Tissue Engineering. Tissue Engineering: Part C Methods, 15, 115-124.
http://dx.doi.org/10.1089/ten.tec.2008.0288
[4] Kim, B.S., Nikolovski, J., Bonadio, J., Smiley, E. and Mooney, D.J. (1999) Engineered Smooth Muscle Tissues: Regulating Cell Phenotype with the Scaffold. Experimental Cell Research, 251, 318-328.
http://dx.doi.org/10.1006/excr.1999.4595
[5] Ueki, K., Takazakura, D., Marukawa, K., Shimada, M., Nakagawa, K., Takatsuka, S. and Yamamoto, E. (2003) The Use of Polylactic Acid/Polyglycolic Acid Copolymer and Gelatin Sponge Complex Containing Human Recombinant Bone Morphogenetic Protein-2 Following Condylectomy in Rabbits. Journal of Cranio-Maxillofacial Surgery, 31, 107-114.
http://dx.doi.org/10.1016/S1010-5182(02)00187-7
[6] Chen, G., Sato, T., Ohgushi, H., Ushida, T., Tateishi, T. and Tanaka, J. (2005) Culturing of Skin Fibroblasts in a Thin PLGA-Collagen Hybrid Mesh. Biomaterials, 26, 2559-2266.
http://dx.doi.org/10.1016/j.biomaterials.2004.07.034
[7] Alvarez, K. and Nakajima, H. (2009) Metallic Scaffolds for Bone Regeneration. Materials, 2, 790-832.
http://dx.doi.org/10.3390/ma2030790
[8] Jacobs, J.J., Skipor, A.K., Patterson, L.M., Hallab, N.J., Paprosky, W.G., Black, J. and Galante, J.O. (1998) Metal Release in Patients Who Have Had a Primary Total Hip Arthroplasty. A Prospective, Controlled, Longitudinal Study. The Journal of Bone and Joint Surgery, 80, 1447-1458.
[9] Bar?o, V.A.R., Mathew, M.T., Assun??o, W.G., Yuan, J.C.-C., Wimmer, M.A. and Sukotjo, C. (2012) Stability of cpTi and Ti-6Al-4V Alloy for Dental Implants as a Function of Saliva pH—An Electrochemical Study. Clinical Oral Implants Research, 23, 1055-1062.
http://dx.doi.org/10.1111/j.1600-0501.2011.02265.x
[10] Sarmiento-Gonzalez, A., Marchante-Gayon, J.M., Tejerina-Lobo, J.M., Paz-Jimenez, J. and Sanz-Medel, A. (2008) High-Resolution ICP-MS Determination of Ti, V, Cr, Co, Ni, and Mo in Human Blood and Urine of Patients Implanted with a Hip or Knee Prosthesis. Analiticaland Bioanalitical Chemistry, 391, 2583-2589.
http://dx.doi.org/10.1007/s00216-008-2188-4
[11] Van Bae, S., Kerckhofs, G., Moesen, M., Pyka, G., Schrooten, J. and Kruth, J.P. (2011) Micro-CT-Based Improvement of Geometrical and Mechanical Controllability of Selective Laser Melted Ti6Al4V Porous Structures. Materials Science and Engineering: A, 528, 7423-7431.
http://dx.doi.org/10.1016/j.msea.2011.06.045
[12] Van Bael, S., Chai, Y.C., Truscello, S., Moesen, M., Kerckhofs, G., Van Oosterwyck, H., Kruth, J.-P. and Schrooten, J. (2012) The Effect of Pore Geometry on the in Vitro Biological Behavior of Human Periosteum-Derived Cells Seeded on Selective Laser-Melted Ti6Al4V Bone Scaffolds. Acta Biomateialia, 8, 2824-2834.
http://dx.doi.org/10.1016/j.actbio.2012.04.001
[13] Mullen, L., Stamp, R.C., Fox, P., Jones, E., Ngo, C. and Sutcliffe, C.J. (2010) Selective Laser Melting: A Unit Cell Approach for the Manufacture of Porous, Titanium, Bone In-Growth Constructs, Suitable for Orthopedic Applications. II. Randomized Structures. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 92, 178-188.
http://dx.doi.org/10.1002/jbm.b.31504
[14] Murr, L.E., Gaytan, S.M., Martinez, E., Medina, F. and Wicke, R.B. (2012) Next Generation Orthopaedic Implants by Additive Manufacturing Using Electron Beam Melting. International Journal of Biomaterials, 2012, 1-14.
http://dx.doi.org/10.1155/2012/245727
[15] Korpela, J., Kokkari, A., Korhonen, H., Malin, M., Narhi, T. and Seppala, J. (2013) Biodegradable and Bioactive Porous Scaffold Structures Prepared Using Fused Deposition Modeling. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 101, 610-619.
http://dx.doi.org/10.1002/jbm.b.32863
[16] Wong, K.C., Kumta, S.M., Sze, K.Y. and Wong, C.M. (2012) Use of a Patient-Specific CAD/CAM Surgical Jig in Extremity Bone Tumor Resection and Custom Prosthetic Reconstruction. Computer Aided Surgery, 17, 284-293.
http://dx.doi.org/10.3109/10929088.2012.725771
[17] Att, W., Hori, N., Takeuchi, M., Ouyang, J., Yang, Y., Anpo, M. and Ogawa, T. (2009) Time-Dependent Degradation of Titanium Osteoconductivity: An Implication of Biological Aging of Implant Materials. Biomaterials, 30, 5352-5363.
http://dx.doi.org/10.1016/j.biomaterials.2009.06.040
[18] Aita, H., Hori, N., Takeuchi, M., Suzuki, T., Yamada, M., Anpo, M. and Ogawa, T. (2009) The Effect of Ultraviolet Functionalization of Titanium on Integration with Bone. Biomaterials, 30, 1015-1025.
http://dx.doi.org/10.1016/j.biomaterials.2008.11.004
[19] Bunyaratavej, P. and Wang, H. L. (2001) Collagen Membranes: A Review. Journal of Periodontology, 72, 215-229.
http://dx.doi.org/10.1902/jop.2001.72.2.215
[20] Stavropoulos, A., Sculean, A. and Karring, T. (2004) GTR Treatment of Intrabony Defects with PLA/PGA Copolymer or Collagen Bioresorbable Membranes in Combination with Deproteinized Bovine Bone (Bio-Oss). Clinical Oral Investigations, 8, 226-232.
http://dx.doi.org/10.1007/s00784-004-0277-0
[21] Teparat, T., Solt, C.W., Claman, L.J. and Beck, F.M. (1998) Clinical Comparison of Bioabsorbable Barriers with NonResorbable Barriers in Guided Tissue Regeneration in the Treatment of Human Intrabony Defects. Journal of Periodontology, 69, 632-641.
http://dx.doi.org/10.1902/jop.1998.69.6.632

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