Cell Proliferation Ability of Mouse Fibroblast-Like Cells and Osteoblast-Like Cells on a Ti-6Al-4V Alloy Film Produced by Selective Laser Melting
Mayu Kawase, Tatsuhide Hayashi, Masaki Asakura, Akimichi Mieki, Hironari Fuyamada, Masahiro Sassa, Shizuka Nakano, Masashi Hagiwara, Toru Shimizu, Tatsushi Kawai
Advanced Laser and Process Technology Research Association (ALPROT), Tokyo, Japan; Laser Sintering Department, ASPECT Inc., Tokyo, Japan.
Advanced Laser and Process Technology Research Association (ALPROT), Tokyo, Japan; Low-Formability-Materials Processing Group, Advanced Manufacturing Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan.
Department of Dental Materials Science, Aichi Gakuin University School of Dentistry, Nagoya, Japan.
Department of Dental Materials Science, Aichi Gakuin University School of Dentistry, Nagoya, Japan; Advanced Laser and Process Technology Research Association (ALPROT), Tokyo, Japan.
Department of Gerodontology, Aichi Gakuin University School of Dentistry, Nagoya, Japan.
DOI: 10.4236/msa.2014.57051   PDF   HTML     3,247 Downloads   4,596 Views   Citations


Successful regeneration of tissues and organs relies on the application of suitable substrates or scaffolds in scaffold-based regenerative medicine. In this study, Ti-6Al-4V alloy films (Ti alloy film) were produced using a three-dimensional printing technique called Selective Laser Melting (SLM), which is one of the metal additive manufacturing techniques. The thickness of produced Ti alloy film was approximately 250 μm. The laser-irradiated surface of Ti alloy film had a relatively smooth yet porous surface. The non-irradiated surface was also porous but also retained a lot of partially melted Ti-6Al-4V powder. Cell proliferation ability of mouse fibroblast-like cells (L929 cells) and mouse osteoblast-like cells (MC3T3-E1 cells) on both the surfaces of Ti alloy film was examined using WST assay. Both L929 and MC3T3-E1 cells underwent cell proliferation during the culture period. These results indicate that selective laser melting is suitable for producing a cell-compatible Ti-6Al-4V alloy film for biomaterials applications.

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Kawase, M. , Hayashi, T. , Asakura, M. , Mieki, A. , Fuyamada, H. , Sassa, M. , Nakano, S. , Hagiwara, M. , Shimizu, T. and Kawai, T. (2014) Cell Proliferation Ability of Mouse Fibroblast-Like Cells and Osteoblast-Like Cells on a Ti-6Al-4V Alloy Film Produced by Selective Laser Melting. Materials Sciences and Applications, 5, 475-483. doi: 10.4236/msa.2014.57051.

Conflicts of Interest

The authors declare no conflicts of interest.


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