Dynamism of Antimicrobial Activity of Actinomycetes—A Case Study from Undisturbed Microbial Niche

Jayshree D. Patel, Mahesh Parmar, Purshottam Patel, Pravin Rohit, Ramesh Taviyad, Parveen Ansari, Bipasha Bhattacharya, Dhaval Vyas, Vipin Kumar, Nirmal S. Sahay, Pawan K. Singh

National Innovation Foundation India, Satellite Complex, Nr. Mansi Circle, Ahmedabad, Gujarat, India.
Sadbhav SRISTI Sanshodhan Laboratory, AES Boys Hostel Campus, Nr. Gujarat University Library & SBI Bank, Navrangpura, Ahmedabad, Gujarat, India.

DOI: 10.4236/aim.2014.46039                

Abstract

Escalation of drug resistant microbes (bacteria) had forced researchers to search new and improved therapeutic compounds from different possible sources, including metabolites secreted by the actinomycetes. The aim of this study was to evaluate the pattern of antimicrobial actinomycetes from physiologically distinct soil of different geographical locations. Forty five soil samples were collected from 5 districts of Gujarat including two sanctuaries as source of survey for bioactive actinomycetes. Crowded plate technique was used for isolation and Agar cylinder method was employed for the antimicrobial screening. A total of 171 actinomycetes were isolated and screened against eighteen pathogens responsible for causing diseases in plants and humans. Results indicate that 79% of the isolates were active against at least one of the eighteen tested pathogens. Some of the actinomycetes strain had shown strong antibacterial and antifungal activity which may be a good source of obtaining novel antimicrobials.

Keywords

Actinomycetes, Screening, Antimicrobial, Drug Resistance, Soil Microflora

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Vining, L.C. (1992) Secondary Metabolism, Inventive Evolution and Biochemical Diversity―A Review. Gene, 115, 135-140. http://dx.doi.org/10.1016/0378-1119(92)90551-Y
[2]	Edwards, C. (1993) Isolation, Properties and Potential Application of Thermophilicactinomycetes. Applied Biochemistry and Biotechnology, 42, 161-179.
[3]	Demain, A.L. (1995) Why Do Microorganisms Produce Antimicrobials? In: Hunter, P.A., Darby, G.K. and Russell, N.J., Eds., Fifty Years of Antimicrobials: Prospective and Future Trends-Symposium 53, Society of General Microbiology, Cambridge University Press, Cambridge, 205-228.
[4]	Jiang, Y., Chen, X., Lou, K. and Mao, P. (2013) Cultivable Soil Actinomycete Communities in Some Areas of Western China. Academy Journal of Biotechnology, 1, 1-13.
[5]	Basilio, A., Gonzalez, I., Vicente, M.F., Gorrochategui, J., Cabello, A., Gonzalez, A. and Genilloud, O. (2003) Patterns of Antimicrobial Activities from Soil Actinomycetes Isolated under Different Conditions of pH and Salinity. Journal of Applied Microbiology, 95, 814-823. http://dx.doi.org/10.1046/j.1365-2672.2003.02049.x
[6]	Moncheva, P., Tishkov, S., Dimitrova, N., Chipeva, V., Antonova-Nikolova, S. and Bogatzevska, N. (2000-2002) Characteristics of Soil Actinomycetes from Antarctica. Journal of Culture Collections, 3, 3-14.
[7]	Miyadoh, S. (1997) Frontispiece. In: Miyadoh, S., Ed., Atlas of Actinomycetes, The Society for Actinomycetes Japan, Asakura Co., 1-5.
[8]	Sahin, N. and Ugur, A. (2003) Investigation of the Antimicrobial Activity of Some Streptomyces Isolates. Turkish Journal of Biology, 27, 79-84.
[9]	Kurtboke, D.I. (2005) Actinophages as Indicator of Actinomycete Taxa in Marine Environments. Anton van Leeuw, 87, 19-28. http://dx.doi.org/10.1007/s10482-004-6535-y
[10]	Aghighi, S., Shahidi Bonjar, G.H., Rawashdeh, R., Batayneh, S. and Saadoun, I. (2004) First Report of Antifungal Spectra of Activity of Iranian Actinomycetes Strains against Alternariasolani, A. alternate, Fusariumsolani, Phytophthoramegasperma, Verticilliumdahliea and Saccharomyces cerevisiae. Asian Journal of Plant Sciences, 3, 463-471. http://dx.doi.org/10.3923/ajps.2004.463.471
[11]	Vali, S., Suvathi, S.S., Ayusha, O.S., Nirmala, P., Vinoth, K.P. and Reena, A. (2012) Antimicrobial Potential of Actinomycetes Species Isolated from Marine Environment. Asian Pacific Journal of Tropical Biomedicine, 469-473.
[12]	Nanjwade, B.K., Chandrashekhara, S., Shamarez, A.M., Goudanavar, P.K. and Manvi, F.V. (2010) Isolation and Morphological Characterization of Antibiotic Producing Actinomycetes. Tropical Journal of Pharmaceutical Research, 9, 231-236.
[13]	Ouhdouch, Y., Barakate, M. and Finance, C. (2001) Actinomycetes of Moroccan Habitats: Isolation and Screening for Antifungal Activities. European Journal of Soil Biology, 37, 69-74. http://dx.doi.org/10.1016/S1164-5563(01)01069-X
[14]	Tortorano, A.M., Cabrini, E. and Vivani, M.A. (1979) Sensibilite in Vitro des Levuresacinq Antibiotiques. Comparaison de deuxmethodes C.M.I. en gelose et methode des disques. Bulletin of Social Frame Mycal Medicine, 8, 69-74.
[15]	Oskay, M., Tamer, A.U. and Azeri, C. (2004) Antibacterial Activity of Some Actinomycetes Isolated from Farming Soils of Turkey. African Journal of Biotechnology, 3, 441-446.
[16]	Ceylan, O., Okmen, G. and Ugur, A. (2008) Isolation of Soil Streptomyces as Source Antibiotics Active against Antibiotic-Resistant Bacteria. European and Asian Journal of BioSciences, 2, 73-82.
[17]	Gurung, T.D., Sherpa, C., Agrawal, V.P. and Lekhak, B. (2009) Isolation and Characterization of Antibacterial Actinomycetes from Soil Samples of Kalapatthar, Mount Everest Region. Nepal Journal of Science and Technology, 10, 173-182.
[18]	Kuchari, M.G.A. (1999) The Isolation of Halotolerant Pigmented Actinomycetes from Jeddah Sea Shore, Saudi Arabia. Journal of King Abdulaziz University, 11, 5-12.
[19]	Lam, K.S. (2006) Discovery of Novel Metabolites from Marine Actinomycetes. Current Opinion in Microbiology, 9, 245-251. http://dx.doi.org/10.1016/j.mib.2006.03.004
[20]	Bredholt, H., Fjaervic, E., Johnsen, G. and Zotchev, S.B. (2008) Actinomycetes from Sediments in the Trondheim Fjord, Norway: Diversity and Biological Activity. Marine Drugs, 6, 12-24. http://dx.doi.org/10.3390/md6010012
[21]	Suthindhiran, K. and Kannabiran, K. (2009) Cytotoxic and Antimicrobial Potential of Actinomycetes Species Saccharopolyspora salina VITSDK4 Isolated from the Bay of Bengal Coast of India. American Journal of Infectious Diseases, 5, 90-98. http://dx.doi.org/10.3844/ajidsp.2009.90.98
[22]	Araujo, J.M., Silva, A.C. and Azevedo, J.L. (2000) Isolation of Endophytic Actinomycetes from Roots and Leaves of Maize (Zea mays L.). Brazilian Archives of Biology and Technology, 43, 447-451. http://dx.doi.org/10.1590/S1516-89132000000400016
[23]	Inderiati, S. and Franco, C.M.M. (2008) Isolation and Identification of Endophytic Actinomycetes and Their Antifungal Activity. Jounal of Biotechnology Research in Tropical Region, 1, 1-6.
[24]	Watanabe, Y., Shinzato, N. and Fukatsu T. (2003) Isolation of Actinomycetes from Termites’ Guts. Bioscience, Biotechnology and Biochemistry, 67, 1797-1801. http://dx.doi.org/10.1271/bbb.67.1797
[25]	Skoko, N., Vujovic, J., Savic, M., Papic, N., Vasiljevic, B. and Ljubijankic, G. (2005) Construction of Saccharomyces cerevisiae Strain FAV20 Useful in Detection of Immunosuppressants Produced by Soil Actinomycetes. Journal of Microbiological Methods, 61, 137-140. http://dx.doi.org/10.1016/j.mimet.2004.11.007
[26]	Busti, E., Monciardini, P., Cavaletti, L., Bamonte, R., Lazzarini, A., Sosio, M. and Donadio, S. (2006) Antibiotic-Producing Ability by Representatives of a Newly Discovered Lineage of Actinomycetes. Microbiology, 152, 675-683. http://dx.doi.org/10.1099/mic.0.28335-0
[27]	Sessitsch, A., Weilharter, A., Gerzabek, M.H., Kirchmann, H. and Kandeler, E. (2001) Microbial Population Structures in Soil Particle Size Fractions of a Long-Term Fertilizer Field Experiment. Applied and Environmental Microbiology, 67, 4215-4224. http://dx.doi.org/10.1128/AEM.67.9.4215-4224.2001
[28]	Vijaykumar, R., Muthukumar, C., Thajuddin, N., Panneerselvam, A. and Saravanamuthu, R. (2007) Studies on the Diversity of Actinomycetes in the Palk Strait Region of Bay of Bangal, India. Actinomycetologica, 21, 59-65. http://dx.doi.org/10.3209/saj.SAJ210203
[29]	Hong, K., Gao, A.H., Xie, Q.Y., Gao, H., Zhuang, L., Lin, H.P., Yu, H.P., Li, J., Yao, X.S., Goodfellow, M. and Ruan, J.S. (2009) Actinomycetes for Marine Drug Discovery Isolated from Mangrove Soils and Plants in China. Marine Drugs, 7, 24-44. http://dx.doi.org/10.3390/md7010024
[30]	Pelaez, F. and Genilloud, O. (2003) Discovering New Drugs from Microbial Natural Products. In: Barredo, J.L., Ed., Microorganisms for Health Care, Foods and Enzyme Production, Research Signpost, Trivandrum, 1-22.