Histopathology and Immunohistochemistry Assessments of Acute Experimental Infection by Brucella melitensis in Bucks

Nurrul Shaqinah Nasruddin; Mazlina Mazlan; Mohd Zamri Saad; Hazilawati Hamzah; Jasni Sabri

doi:10.4236/ojpathology.2014.42009

Home > Journals > Medicine & Healthcare > OJPathology

Open Journal of Pathology > Vol.4 No.2, April 2014

Histopathology and Immunohistochemistry Assessments of Acute Experimental Infection by Brucella melitensis in Bucks ()

Nurrul Shaqinah Nasruddin, Mazlina Mazlan, Mohd Zamri Saad, Hazilawati Hamzah, Jasni Sabri

Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Selangor, Malaysia.
Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kelantan, Malaysia.
Research Centre for Ruminant Diseases, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Selangor, Malaysia.

DOI: 10.4236/ojpathology.2014.42009 PDF HTML 3,965 Downloads 6,412 Views Citations

Abstract

Background: Brucellosis in male goats is characterized by arthritis, orchitis and epididymitis, which may induce infertility. Nevertheless, these lesions were categorized as chronic while acute lesions had not been described. This study investigates the histopathological and immuno histochemistry reactions in organs of bucks acutely infected by Brucella melitensis. Results: Only testis and prepuce of acutely infected bucks showed significantly severe histological lesions. Other internal organs had mild to moderate lesions. However, positive immunohistochemistry stainings were observed in organs except the bulbourethral gland. There was a significant positive correlation between the distribution of B. melitensis and IHC intensity but no significant correlation between the IHC intensity and histopathology lesions. Conclusion: The results indicate that acute brucellosis did not lead to clinical presentation, although B. melitensis was well distributed in various organs of bucks.

Keywords

Histopathology, Immunohistochemistry, Brucella melitensis, Bucks

Share and Cite:

Nasruddin, N. , Mazlan, M. , Saad, M. , Hamzah, H. and Sabri, J. (2014) Histopathology and Immunohistochemistry Assessments of Acute Experimental Infection by Brucella melitensis in Bucks. Open Journal of Pathology, 4, 54-63. doi: 10.4236/ojpathology.2014.42009.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Seleem, M.N., Boyle, S.M. and Sriranganathan, N. (2010) Brucellosis: A Re-Emerging Zoonosis. Veterinary Microbiology, 140, 392-398. http://dx.doi.org/10.1016/j.vetmic.2009.06.021
[2]	Gupta, V.K., Rout, P.K. and Vihan, V.S. (2007) Induction of Immune Response in Mice with a DNA Vaccine Encoding Outer Membrane Protein (omp31) of Brucella melitensis 16M. Research in Veterinary Science, 82, 305-313. http://dx.doi.org/10.1016/j.rvsc.2006.07.014
[3]	Abdullah, F.F.J., Adamu, L., Hazirah, N., Osman, A. Y., Mansor, R., Harun, A.W., Zamri-Saad, M., Omar, A.R. and Saharee, A.Z. (2013) Clinical and Reproductive Pathological Changes Associated with Brucella melitensis and Its Lipopolysaccharides in Female Mice via Oral Inoculation. American Journal of Animal and Veterinary Science, 8, 104-111. http://dx.doi.org/10.3844/ajavsp.2013.104.111
[4]	Neta, A.V.C., Mol, J.P.S., Xavier, M.N., Paixao, T.A., Lage, A.P. and Santos, R.L. (2010) Pathogenesis of Bovine Brucellosis. Veterinary Journal, 184, 146-155. http://dx.doi.org/10.1016/j.tvjl.2009.04.010
[5]	Adams, L.G. (2002) The Pathology of Brucellosis Reflects the Outcome of the Battle between the Host Genome and the Brucella Genome. Veterinary Microbiology, 90, 553-561. http://dx.doi.org/10.1016/S0378-1135(02)00235-3
[6]	Xavier, M.N., Paixao, T.A., Hartigh, A.B., Tsolis, R.M. and Santos, R.L. (2010) Pathogenesis of Brucella spp. Veterinary Science Journal, 4, 109-118.
[7]	Ilhan, F. and Yener, Z. (2008) Immunohistochemical Detection of Brucella melitensis Antigens in Cases of Naturally Occurring Abortions in Sheep. Journal of Veterinary Diagnostic Investigation, 20, 803-806. http://dx.doi.org/10.1177/104063870802000616
[8]	Xavier, M.N., Paixa~o, T.A., Poester, F.P., Lage, A.P. and Santos, R.L. (2009) Pathological, Immunohistochemical and Bacteriological Study of Tissues and Milk of Cows and Fetuses Experimentally Infected with Brucella abortus. Journal of Comparative Pathology, 140, 149-157. http://dx.doi.org/10.1016/j.jcpa.2008.10.004
[9]	Emikpe, B.O., Tanko, P.N., Onilude, O.M. and Sabri, M.Y. (2013) The Influence of Dexamethasone Treatment and Successive Road Transport Stress on the Occurrence of Caprine Pneumonia in a Hot Humid Tropical Environment. Veterinary World, 6, 497-501.
[10]	Plumeriastuti, H. and Zamri-Saad, M. (2012) Detection of Brucella melitensis in Seropositive Goats. Online Journal of Veterinary Research, 16, 1-7.
[11]	McFarland, J. (1907) An Instrument for Estimating the Number of Bacteria in Suspensions Used for Calculating the Opsonic Index and Vaccines. Journal of American Medical Information Association, 14, 1176-1178. http://dx.doi.org/10.1001/jama.1907.25320140022001f
[12]	Suraud, V., Jacques, I., Olivier, M. and Guilloteau, L.A. (2008) Acute Infection by Conjunctival Route with Brucella melitensis Induces IgG+ Cells and IFN-γ Producing Cells in Peripheral and Mucosal Lymph Nodes in Sheep. Microbes and Infection, 10, 1370-1378. http://dx.doi.org/10.1016/j.micinf.2008.08.003
[13]	Carvalho Junior, C.A., Moustacas, V.S., Xavier, M.N., Costa, E.A., Costa, L.F., Silva, T.M.A., Paixao, T.A., Borges, A.M., Gouveia, A.M.G. and Santos, R.L. (2012) Andrological, Pathologic, Morphometric, and Ultrasonographic Findings in Bucks Experimentally Infected with Brucella ovis. Small Ruminant Research, 102, 213-222. http://dx.doi.org/10.1016/j.smallrumres.2011.08.004
[14]	Gouletsou, P.G., Fthenakis, G.C., Cripps, P.J., Papaioannou, N., Lainas, T., Psalla, D. and Amiridis, G.S. (2004) Experimentally Induced Orchitis Associated with Arcanobacterium pyogenes: Clinical, Ultrasonographic, Seminological and Pathological Features. Theriogenology, 62, 1307-1328.
[15]	Celli, J. (2006) Surviving Inside a Macrophage: The Many Ways of Brucella. Research in Microbiology, 157, 93-98.
[16]	Jansen, B.C. (1980) The Pathology of Bacterial Infection of the Genitalia in Bucks. Onderstepoort Journal of Veterinary Research, 47, 263-267.
[17]	Mense, M.G., Richard, H., Borschel, R.H., Wilhelmsen, C.L., Louise Pitt, M.L. and Hoover, D.L. (2004) Pathologic Changes Associated with Brucellosis Experimentally Induced by Aerosol Exposure in Rhesus Macaques (Macaca mulatta). American Journal of Veterinary Research, 65, 644-652. http://dx.doi.org/10.2460/ajvr.2004.65.644
[18]	Colmenero Jde, D., Queipo-Ortuno, M.I., Maria Reguera, J., Angel Suarez-Munoz, M., Martín-Carballino, S. and Morata, P. (2002) Chronic Hepatosplenic Abscesses in Brucellosis. Clinico-Therapeutic Features and Molecular Diagnostic Approach. Diagostic Microbiology and Infectious Diseases, 42, 159-167.
[19]	OIE (2009) Caprine and Ovine Brucellosis (Excluding Brucella ovis). Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.07.02_caprine_Ovine_bruc.pdf (accessed 07.10.13).
[20]	Gwida, M., El-Gohary, A., Melzer, F., Khan, I., Rosler, U. and Neubauer, H. (2012) Brucellosis in Camels. Research in Veterinary Science, 92, 351-355. http://dx.doi.org/10.1016/j.rvsc.2011.05.002
[21]	Poester, F.P., Nielsen, K., Samartino, L.E. and Yu, W.L. (2010) Diagnosis of Brucellosis. Open Veterinary Science Journal, 4, 46-60.