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Expression of Telomerase Reverse Transcriptase during the Malignant Transformation of Cadmium-Induced Cells

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DOI: 10.4236/gep.2014.22018    2,954 Downloads   3,807 Views  


The objective of the present study was to investigate human telomerase reverse transcriptase (hTERT) mRNA and protein expressions during the cadmium chloride-induced malignant transformation of human bronchial epithelial (16HBE) cells. Fluorescence quantitative PCR (FQ-PCR) and Western blot analyses were performed to detect the hTERT mRNA and protein expressions in normal 16HBE cells, cadmium chloride-transformed 16HBE cells, and tumorigenic cells from nude mice inoculated with cadmium chloride-transformed 16HBE cells. Under the inner standard of GAPDH, the hTERT mRNA expression was significantly higher at different stages of malignant transformation (cadmium chloride-transformed 16HBE cells at passages 15 and 35 and tumorigenic cells from nude mice) than in normal 16HBE cells, and increased with the development of malignancy (P < 0.01). In addition, hTERT protein expression increased with the development of malignancy. These findings demonstrate that hTERT expression is related to cadmium chlorideinduced malignant transformation. Cadmium chloride-induced malignant transformation is involved in changes in the hTERT activity, and might be an early event in cadmium chloride-induced malignant transformation.

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The authors declare no conflicts of interest.

Cite this paper

Wang, M. and Lei, Y. (2014) Expression of Telomerase Reverse Transcriptase during the Malignant Transformation of Cadmium-Induced Cells. Journal of Geoscience and Environment Protection, 2, 129-133. doi: 10.4236/gep.2014.22018.


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