Antinociceptive Effects of Pomegranate (Punica granatum L.) Juice and Seed Extracts on Acute Corneal Pain in Mice


In the present study, the antinociceptive effects of acute (2, 4 and 6 ml/kg) and chronic (1, 2 and 3 ml/kg for 14 days)oral administration of pomegranate (Punica granatum L.) juice and seed extract with or without morphine and naloxane were investigated on hypertonic saline-induced acute corneal pain perception in mice. The number of eye wipes with a forelimb was counted for a period of 30 seconds as the criterion for pain assessment. Acute oral administration of the extract (at 6 ml/kg dose, once) and chronic oral administration (at 2 and 3 ml/kg for 14 days each) significantly decreased the number of eye wipes after subcutaneous injection of morphine (2 mg/kg, sc), naloxone (2 mg/kg, sc) and normal saline (2 mg/kg, sc) compared with control (p < 0.05). The morphine-induced antinociception was significantly improved by both acute and chronic oral administrations of pomegranate extract (p < 0.05). Naloxone (2 mg/kg, sc) did not reverse the antinociceptive effects of acute (at 6 ml/kg dose, once, oral) and chronic (at 2 and 3 ml/kg for 14 days each) treatments. These findings demonstrate that acute high-dose and long-term lower-dose of pomegranate juice and seed extract can decrease acute corneal pain and improve morphine-induced antinociception in mice.

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Malek, Z. , Dara, S. and Jahromy, M. (2014) Antinociceptive Effects of Pomegranate (Punica granatum L.) Juice and Seed Extracts on Acute Corneal Pain in Mice. World Journal of Neuroscience, 4, 99-105. doi: 10.4236/wjns.2014.42012.

Conflicts of Interest

The authors declare no conflicts of interest.


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