Genotoxicity Assessment of Contaminated Drinking Water Sources in a Rural Community in Edo State of Nigeria

DOI: 10.4236/gep.2014.22009   PDF   HTML     5,263 Downloads   6,622 Views   Citations


In most rural settlements in Nigeria, provision of potable water for drinking and domestic purposes is a big challenge; therefore analysis of drinking water is of great importance as contaminated water jeopardizes both the physical and social health of all people. Water samples were obtained during the dry and wet seasons from a borehole and a man-made lake constructed through self-help effort in Obazuwa community in Ovia North East Local Government Area of Edo State, Nigeria. They were analyzed for physicochemical parameters and subjected to cyto-genotoxic evaluation using the Allium cepa assay. Results of the physicochemical analysis showed that most of the parameters (pH, chromium, copper, chlorides, nickel, iron, zinc, cadmium, lead and manganese) of the lake water in both seasons exceeded World Health Organisation (WHO) permissible limits. Total heterotrophic bacteria and E. coli were present with dry season water samples having higher amounts. Compared to the control, the mitotic index decreased significantly (p < 0.05) in the water samples and were characterized by a number of chromosomal aberrations notably bridges, fragments, sticky chromosomes, disoriented chromosomes, and micronuclei in significant amounts and these were more pronounced in water samples obtained during the dry season. The findings in this study are of public health relevance as access to safe water is a fundamental human need and therefore, a basic human right.

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Olorunfemi, D. , Olorunfemi, O. and Agbozu, I. (2014) Genotoxicity Assessment of Contaminated Drinking Water Sources in a Rural Community in Edo State of Nigeria. Journal of Geoscience and Environment Protection, 2, 52-59. doi: 10.4236/gep.2014.22009.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] American Public Health Association (APHA) (1998). Standard Methods for the Examination of Water and Wastewater (18th ed., pp. 45-60). Washington DC: American Public Health Association.
[2] American Public Health Association (APHA) (2005). Standard Methods for the Examination of Water and Wastewater (21st ed., 1220 p). Washington DC: American Public Health Association.
[3] Anderson, O. (1985). Evaluation of the Spindle Inhibition Effects of Ni2+ Quantitation of Chromosomal Super Condensation. Research Communications in Chemical Pathology & Pharmacology, 50, 379-384.
[4] Arkhipchuk, V. V., Malinovskaya, M. V., & Garanko, N. N. (2000). Cytogenetic Study of Organic and Inorganic Toxic Substances on Allium cepa, Lactuca sativa and Hydra attenuate Cells. Environmental Toxicology, 15, 338-344.<338::AID-TOX10>3.0.CO;2-R
[5] Babatunde, B. B., & Bakare, A. A. (2006). Genotoxicity Screening of Waste Water from Agbara Industrial Estate, Nigeria Evaluated with the Allium cepa Test. Poll Results, 25, 227-234.
[6] Barcelona, M., Gibb, J. P., Helfrich, J. A., & Garske, E. E. (1985). Practical Guide for Groundwater Sampling (374 p). Ilinois State Water Survey ISWS Contract Report, Illinois.
[7] Chauhan, L. K. S., Saxena, P. N., Sundararaman, V., & Gupta, S. K. (1998). Diuron Induced Cytological and Ultrastructural Alterations in the Root Meristem Cells of Allium cepa. Pesticide Biochemistry and Physiology, 62, 152-163.
[8] Claaasen, H. C. (1982). Guidelines and Techniques for Obtaining Water Samples That Accurately Represent the Quality of an Aquifer (49 p). US Geological Survey Open File Report 82-1024.
[9] Fawole, O. O., Yekeen, T. A., Ayandele, A. A., Akinboro, A., Azeez, M. A., & Adewoye, S. O. (2008). Polluted Alamuyo River: Impacts on Surrounding Wells, Microbial Attributes and Toxic Effects on Allium cepa Root Cells. African Journal of Biotechnology, 7, 450-458.
[10] FiskesjÖ, G. (1993). The Allium Test in Wastewater Monitoring. Environmental Toxicology and Water Quality, 8, 291-298. Http://
[11] FiskesjÖ, G. (1997). Allium Test for Screening Chemicals: Evaluation of Cytologic Parameters. In W. Wang, J. W. Gorsuch, & J. S. Hughes (Eds.), Plants for Environmental Studies (pp. 308-333). Boca Raton, New York: CRC Lewis Publishers.
[12] Godet, F., Babut, M., Burnel, D., Veber, A. M., & Vasseur, P. (1993). The Genotoxicity of Iron and Chromium in Electroplating Effluents. Mutation Research, 370, 19-28.
[13] G?mürgen, A. N. (2005). Cytological Effect of the Potassium Metabisulphite and Potassium Nitrate Food Preservative on Root Tip of Allium cepa L. Cytologia, 70, 119-128.
[14] Grant, W. F. (1982). Chromosome Aberration Assays in Allium. A Report of the United States Environmental Protection Agency Gene Toxicity Program. Mutation Research, 99, 273-291.
[15] Gupta, S. K., Gupta, R. C., & Gupta, A. B. (2001). Recurrent Diarrhea in Children Living in Areas with High Levels of Nitrate in Drinking Water. Archives of Environmental Health, 56, 369-373.
[16] Ma, T.-H., Anderson, V. A., Harris, M. M., Neas, R. E., & Lee, T. S. (1985). Mutagenicity of Drinking Water Detected by the Tradescantia Micronucleus Test. Canadian Journal of Genetics and Cytology, 27, 143-150.
[17] Okonko, I. O., Adejoye, O. D., Ogunnusi, T. A., Fajobi, E. A., & Shittu, O. (2008). Microbiological and Physicochemical Analysis of Different Water Samples Used for Domestic Purposes in Abeokuta and Ojota, Lagos State, Nigeria. African Journal of Biotechnology, 7, 617-621.
[18] Olorunfemi, D. I., Ofomata, C. R., & Alimba, C. G. (2013). Cytogenotoxicity Assessment of a University Borehole Water Supply Using the Allium cepa Test. Journal of Scientific Research and Development, 14, 25-34.
[19] Olorunfemi, D. I., Okoloko, G. E., Bakare, A. A., & Akinboro, A. (2011). Cytotoxic and Genotoxic Effects of Cassava Effluent Using the Allium cepa Test. Research Journal of Mutagenesis, 1, 1-9.
[20] Onokerhoraye, A. G. (1995). Urbanization and Environment in Nigeria: Implications for Sustainable Development. The Benin Social Science Series for Africa. Benin City: University of Benin.
[21] Schneiderman, M. H., Dewey, W. C., & Highfield, D. P. (1971). Inhibition of DNA Synthesis in Synchronized Chinise Hamster Cells Treated in G1 with Cyclohexamid. Experimental Cell Research, 67, 147-155.
[22] Shugart, L. R., McCarthy, J. F., & Halbrook, R. S. (1992). Biological Markers of Environmental and Ecological Contamination: An Overview. Risk Analysis, 12, 353-360.
[23] Sia Su, G. (2005). Water-Borne Illness from Contaminated Drinking Water Sources in Close Proximity to a Dumpsite in Payatas, The Philippianes. J. Rural Trop. Pub.Hlth., 4, 43-48.
[24] Sia Su, G. (2007). Impact on Drinking Water Sources in Close Proximity to the Payatas Dumpsite, Philippianes. Journal of Public Health, 15, 51-55.
[25] Standard Organization of Nigeria (SON) (2007). Nigerian Standard for Drinking Water Quality (29 p). Abuja: Lome Street.
[26] Turkoglu, S. (2007). Genotoxicity of Five Food Preservatives Tested on Root Tips of Allium cepa L. Mutation Research, 626, 4-14.
[27] United States Environmental Protection Agency (USEPA) (1996). National Recommended Water Quality Criteria—Correction: EPA 822/Z-99-001. Washington DC: USEPA.
[28] World Health Organization (WHO) (2006). Guidelines for Drinking Water Quality Vol. 1. Geneva: World Health Organization.
[29] World Health Organization (WHO) (2011). Guidelines for Drinking Water Quality (4th ed., 564 p). Geneva: World Health Organization.
[30] Wright, J., Grungy, S., & Conroy, R. (2004). Household Drinking Water in Developing Countries, a Systematic Review of Microbiological Contamination between the Source and Point Use. Tropical Medicine and Health, 8, 106-177.
[31] Yassi, A., Kjellstrom, T., de Kok, T., & Guidotti, T. L. (2001). Basic Environmental Health (456 p). New York: Oxford University Press, Inc.
[32] Zhang, Y., & Yang, X. (1994). The Toxic Effect of Cadmium on Cell Division and Chromosomal Morphology of Hordeum vulgare. Mutation Research, 312, 121-126.

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