Effect of Arbuscular Mycorrhiza Fungal Inoculation with Compost on Yield and Phosphorous Uptake of Berseem in Alkaline Calcareous Soil


An experiment was conducted in pots under natural conditions in alkaline calcareous soil to determine berseem (Trifolium alexandrium) yield and P uptake as affected by Arbuscular mycorrhizal fungi (AMF) inoculation with compost prepared from fresh animal dung and rock phosphate. Data indicated that berseem shoot and roots yields increased significantly (P ≤ 0.05) by inoculation of indigenous mycorrhiza (AMF-I) and half dose of compost. Shoot yield increased as 98% and 76% roots yield as 60% and 52% over control and N and K fertilizers. Maximum and significantly (P ≤ 0.05) increased plant N and P uptake by berseem was observed in the treatment inoculated by commercial mycorrhiza (AMF-II) with full dose of compost followed by the inoculation of AMF-II with half dose of compost. Plants uptake of Cu, Mn and Fe was improved significantly (P ≤ 0.05) by the inoculation of AMF-II with half dose of compost, while Zn uptake was increased in the treatment of AMF-II inoculation with full dose of compost. Maximum and significantly (P ≤ 0.05) increased soil spores density of AMF as 27 spores per 20 g soil was noted by inoculation of AMF-I with half dose of compost, while maximum roots infection intensity in berseem was observed by the inoculation of AMF-I with full dose of compost. Results suggest that inoculation of AMF with compost has potential to improve berseem yields and plants nutrients uptake under given soil conditions.

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Jan, B. , Ali, A. , Wahid, F. , Shah, S. , Khan, A. and Khan, F. (2014) Effect of Arbuscular Mycorrhiza Fungal Inoculation with Compost on Yield and Phosphorous Uptake of Berseem in Alkaline Calcareous Soil. American Journal of Plant Sciences, 5, 1359-1369. doi: 10.4236/ajps.2014.59150.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Menge, J.A. and Timmer, L.W. (1982) Procedure for Inoculation of Plants Vesicular-Arbuscular Mycorrhizal in Laboratory. Green House and Field. In: Schenck, N.C., Ed., Methods and Principles of Mycorrhizal Research, American Phytopathological Society, St. Paul.
[2] Smith, S.E. and Read, D.J. (1997) Mycorrhizal Symbiosis. Elsevier Science, New York.
[3] Williamson, L.C., Ribrioux, S.P.C.P., Fitter, A.H., and Leyser, H.M.O (2001) Phosphate Availability Regulates Root System Architecture in Arabidopsis. Plant Physiology, 126, 875-882.
[4] Mengel, K. and Kirkby, E.A (1987) Principles of Plant Nutrition. International Potash Institute, Berne, 588-594.
[5] Vance, C.P., Uhde-Stone, C. and Allan, D.L. (2003) Phosphorus Acquisition and Use: Critical Adaptations by Plants for Securing a Nonrenewable Resource. New Phytologist, 157, 423-447.
[6] Raghothama, K.G. and Karthikeyan, A.S. (2005) Phosphate Acquisition. In: Lambers, H. and Colmer, T.D., Ed., Root Physiology: From Gene to Function, Springer Netherlands, 37-49.
[7] Harris, J.N., New, P.B. and Martin, P.M. (2006) Laboratory Tests Can Predict Beneficial Effects of Phosphate-Solubilising Bacteria on Plants. Soil Biology and Biochemistry, 38, 1521-1526.
[8] Millner, P.D., Sikora, L.J., Kaufman, D D. and Simpson, M.E. (1998) Agricultural Uses of Biosolids and Other Recyclable Municipal Residues. USDA Agricultural Research Service Conservation Research Report, 9-38.
[9] Stewart, W.M., Hammond, L.L. and Van Kauwenbergh, S.J. (2005) Phosphorus as a Natural Resource. In: Sims, J.T. and Sharpley, A.N., Eds., Phosphorus: Agriculture and the Environment, American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, 3-22.
[10] Cordell, D., Drangert, J.O. and White, S. (2009) The Story of Phosphorus: Global Food Security and Food for Thought. Global Environmental Change, 19, 292-305.
[11] Ranjhan, S.K. (2001) Animal Nutrition in the Tropics. Vikas Publishing House Pvt. Ltd., New Delhi.
[12] Koehler, F.E., Moudre, C.D. and McNeal, B.L. (1984) Laboratory Manual for Soil Fertility. Washington State University, Pulman, 456-461.
[13] Mclean, E.O. (1982) Soil pH and Lime Requirement. In: Page, A.L., Ed., Methods of Soil Analysis. Part 2. Chemical and Microbiological Properties, American Society of Agronomy, Soil Science Society of America, 199-224.
[14] Richards, L.A. (1947) Diagnosis and Improvement of Saline and Alkaline Soils. Soil Science, 64, 432.
[15] Nelson, D.W. and Sommers, L.E. (1996) Total Carbon, Organic Carbon, and Organic Matter. In: Sparks, D.L., Page, A.L., Helmke, P.A., Loeppert, R.H., Soltanpour, P.N., Tabatabai, M.A., Johnston, C.T. and Sumner, M.E., Eds., Methods of Soil Analysis. Part 3-Chemical Methods, Soil Science Society of America Inc., Madison, 961-1010.
[16] Bremner, J.M. (1996) Nitrogen-Total. In: Sparks, D.L., Page, A.L., Helmke, P.A., Loeppert, R.H., Soltanpour, P.N., Tabatabai, M.A., Johnston, C.T. and Sumner, M.E., Eds., Methods of Soil Analysis. Part 3-Chemical Methods, Soil Science Society of America Inc., Madison, 1085-1121.
[17] Soltanpour, P.N. and Schwab, A.P. (1977) A New Soil Test for Simultaneous Extraction of Macro- and Micro-Nuients in Alkaline Soils 1. Communications in Soil Science & Plant Analysis, 8, 195-207.
[18] Hauck, F.W. (1978) China: Recycling of Organic Wastes in Agriculture. Food and Agricultural Organization of the United Nations, Rome.
[19] BioMycTM (2010) Production of AMF Spores and Hyphae. BioMycorrhizal Environment GmbH, Germany.
[20] Walsh, L.M., and Beaton, J.D. (1977) Soil Testing and Plant Analysis. Soil Science Society of America, Inc., Wisconsin.
[21] Barber, S.A. (1995) Soil Nutrient Bioavailability: A Mechanistic Approach. John & Wiley Sons, Inc., New York.
[22] Ryan, M.H., Norton, R.M., Kirkegaard, J.A., McCormick, K.M., Knights, S.E. and Angus, J.F. (2002) Increasing Mycorrhizal Colonisation Does Not Improve Growth and Nutrition of Wheat on Vertosols in South-Eastern Australia. Crop and Pasture Science, 53, 1173-1181.
[23] Jackson, M.L.R. and Barak, P. (2005) Soil Chemical Analysis: Advanced Course. UW-Madison Libraries Parallel Press, USA.
[24] Brundrett, M.C. (1991) Mycorrhizas in Natural Ecosystems. Academic Press, Inc., San Diego.
[25] Phillips, J.M. and Hayman, D.S. (1970) Improved Procedures for Clearing Roots and Staining Parasitic and Vesicular-Arbuscular Mycorrhizal Fungi for Rapid Assessment of Infection. Transactions of the British Mycological Society, 55, 158-161, IN16-IN18.
[26] Koske, R.E. and Gemma, J.N. (1989) A Modified Procedure for Staining Roots to Detect VA Mycorrhizas. Mycological Research, 92, 486-488. http://dx.doi.org/10.1016/S0953-7562(89)80195-9
[27] Schenck, N.C. and Perez, Y. (1990) Manual for Identification of Vesicular Arbuscular Mycorrhizal Fungi. (INVAM). University of Florida, Gainesville.
[28] Steel, R.G.D. and Torrie, J.H. (1980) Principles and Procedures of Statistics, a Biometrical Approach. McGraw-Hill Kogakusha, Ltd., Tokyo.
[29] Habashy, N.R., El-Khair, A.W.A. and Zaki, R.N. (2008) Effect of Organic and Bio-Fertilizers on Phosphorus and Some Micronutrients Availability in a Calcareous Soil. Research Journal of Agricultural and Biological Science, 4, 454-552.
[30] El-Goud, A.K.S.A. (2010) Effect of Endomycorrhizal Fungi and Compost on the Yield and Quality of Maize and Sunflower Plants in Poor Nutrients Soil. PhD Thesis, University of Kassel, Kassel.
[31] El-Sayed, S.E.M., Ramadan, H.M. and El-Fayoumy. M.E. (2006) Impact of Compost and Mineral Fertilizers Application on Cereal Crops in Calcareous Soil. Menofia Journal of Agricultural Research, 31, 1067-1085.
[32] Filho, P.F.M., de F. Vasconcellos, R.L. and Cardoso, E.J.B.N. (2011) Growth and Development of Jack-Bean and Pigeon-Pea in Cassiterirte Mine Spoil. Journal of Soil Science and Environmental Management, 2, 74-79.
[33] Sharif, M. and Jan, B. (2008) Growth and Nutrient Accumulation of Maize Plants as Affected by the Inoculation of AMF with Rock Phosphate. Soil and Environment, 27, 116-122.
[34] Sharif, M., Sarir, M.S., Bakht, J., Saud, S., Ali, A. and Ahmad, M. (2009) Response of Wheat to the Inoculation of Arbuscular Mycorrhizal Fungi in Salt Affected Soil. Sarhad Journal of Agriculture, 25, 209-216.
[35] Lambert, D.H., Baker, D.E. and Cole, H. (1979) The Role of Mycorrhizae in the Interactions of Phosphorus with Zinc, Copper, and Other Elements. Soil Science Society of America Journal, 43, 976-980.
[36] Maksoud, M.A., Haggag, L.F., Azzay, M.A. and Saad, R.N. (1994) Effect of VAM Inoculation and Phosphorous Application on Growth and Nutrient Content (P and K) of Tamarindus indica L. (Tamarind) Seedlings. Annals of Agricultural Science, 30, 355-363.
[37] Schachtman, D.P., Reid, R.J. and Ayling, S.M. (1998) Phosphorus Uptake by Plants: From Soil to Cell. Plant Physiology, 116, 447-453. http://dx.doi.org/10.1104/pp.116.2.447
[38] Ness, R.L.L. and Vlek, P.L.G. (2000) Mechanism of Calcium and Phosphate Release from Hydroxy-Apatite by Mycorrhizal Hyphae. Soil Science Society of America Journal, 64, 949-955.
[39] Celik, I., Ortas, I. and Kilic, S. (2004) Effects of Compost, Mycorrhiza, Manure and Fertilizer on Some Physical Properties of a Chromoxerert Soil. Soil and Tillage Research, 78, 59-67.

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