General Theory of Body Contouring: 1. Processes of Quick and Slow Modulation of Subcutaneous Fat Tissue

Ilja L. Kruglikov

Wellcomet GmbH, Karlsruhe, Germany.

DOI: 10.4236/jcdsa.2014.42016                

Abstract

It is shown that modulation of subcutaneous white adipose tissue (sWAT) volume after application of different physical factors in body contouring procedures cannot be characterized by a single physiological reaction with dispersed reaction times, supposing that several different dynamic processes with different characteristic reaction times must be involved. Quick modulation of sWAT with characteristic times of 10³ - 10⁵ s must be connected with such processes as hyaluronan production and consequent water retention in the tissue, as well as with reversible modulation of its mechanical properties. It is supposed that slow processes with characteristic times of up to 3 × 10⁶ s can be caused by modulation of lipolytic activity of adipocytes through changesin their micro-environment or through their transdifferentiation, as well as through death and removal of adipocytes from sWAT.

Keywords

Body Contouring; Characteristic Reaction Time; Subcutaneous Fat Tissue; Volume Modulation; Hyaluronan

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Spalding, K.L., Arner, E., Westermark, P. O., Bernard, S., Buchholz, B. A., Bergmann, O., Blomqvist, L., Hoffstedt, J., Naslund, E., Britton, T., Concha, H., Hassan, M., Ryden, M., Frisen, J. and Arner, P. (2008) Dynamics of Fat Cell Turnover in Humans. Nature, 453, 783-787. http://dx.doi.org/10. 1038/nature06902
[2]	Arner, E., Westermark, P., Spalding, K.L., Britton, T., Ryden, M., Frisen, J., Bernard, S. and Arner, P. (2010) Adipocyte Turnover: Relevance to Human Adipose Tissue Morphology. Diabetes, 59, 105-109. http://dx.doi.org/10.2337/db09-0942
[3]	Kruglikov, I.L. (2012) Biophysical Basics of Body Treatments. Is Hyaluronan a Link That Has Gone Unnoticed? The American Journal of Cosmetic Surgery, 29, 121-129. http://dx.doi.org/10.5992/AJCS-D-12-00004.1
[4]	Comley, K. and Fleck, N.A. (2010) A Micromechanical Model for the Young’s Modulus of Adipose Tissue. International Journal of Solids and Structures, 47, 2982-2990. http://dx.doi.org/10.1016/j. ijsolstr.2010.07.001
[5]	Comley, K. and Fleck, N.A. (2010) The Toughness of Adipose Tissue: Measurements and Physical Basis. Journal of Biomechanics, 43, 1823-1826. http://dx.doi.org/10.1016/j.jbiomech.2010.02.029
[6]	Geerligs, M., Peters, G.W.M., Ackermans, P.A.J., Oomens, C.W.J. and Baaijens, F.P.T. (2008) Linear Viscoelastic Behavior of Subcutaneous Adipose Tissue. Biorheology, 45, 677-688.
[7]	Geerligs, M., Peters, G.W.M., Ackermans, P.A.J., Oomens, C.W.J. and Baaijens, F.P.T. (2010) Does Subcutaneous Adipose Tissue Behave as an (Anti-)thixotropic Material? Journal of Biomechanics, 43, 1153-1159. http://dx.doi.org/10.1016/j.jbiomech.2009.11.037
[8]	Han, C.Y., Subramanian, S., Chan, C.K., Omer, M., Chiba, T., Wight, T.N. and Chait, A. (2007) Adipocyte-Derived Serum Amyloid A3 and Hyaluronan Play a Role in Monocyte Recruitment and Adhesion. Diabetes, 56, 2260-2273. http://dx.doi.org/10.2337/db07-0218
[9]	Kang, L., Lantier, L., Kennedy, A.J., Bonner, J.S., Mayes, W.H., Bracy, D.P., Bookbinder, L.H., Hasty, A.H., Thompson, C.B. and Wasserman, D.H. (2013) Hyaluronan Accumulates with High Fat Feeding and Contributes to Insulin Resistance. Diabetes, 62, 1888-1896. http://dx.doi.org/10.2337/db12-1502
[10]	Khan, T., Muise, E.S., Iyengar, P., Wang, Z.V., Chandalia, M., Abate, N., Zhang, B.B., Bonaldo, P., Chua, S. and Scherer, P.E. (2009) Metabolic Dysregulation and Adipose Tissue Fibrosis: Role of Collagen VI. Molecular and Cellular Biology, 29, 1575-1591. http://dx.doi.org/10.1128/MCB.01300-08
[11]	Kruglikov, I.L. (2012) The Pathophysiology of Cellulite: Can the Puzzle Eventually Be Solved? Journal of Cosmetics, Dermatological Sciences and Applications, 2, 1-7. http://dx.doi.org/10.4236/jcdsa.2012. 21001
[12]	Rosenwald, M., Perdikari, A., Rülicke, T. and Wolfrum, C. (2013) Bi-Directional Interconversion of Brite and White Adipocytes. Nature Cell Biology, 15, 659-667. http://dx.doi.org/10.1038/ncb2740
[13]	Wang, Q.A., Tao, C., Gupta, R.K. and Scherer, P.E. (2013) Tracking AdipogenesisDuring White Adipose Tissue Development, Expansion and Regeneration. Nature Cell Biology, 19, 1338-1344. http://dx.doi.org/10.1038/nm.3324
[14]	Kruglikov, I.L. (2012) Biophysical Basics of Body Treatments: A Preliminary Study into the Correlation between Washout and Circumference Reduction. The American Journal of Cosmetic Surgery, 29, 196-202.
[15]	Kruglikov, I.L. (2013) Neocollagenesis in Non-Invasive Aesthetic Treatments. Journal of Cosmetics, Dermatological Sciences and Applications, 3, 1-5. http://dx.doi.org/10.4236/jcdsa.2013.31A001
[16]	Wegrowski, Y. (1993) Effect of Hyperthermia on the Extracellular Matrix. I. Heat Enhances Hyaluronan and Inhibits Sulphated Glycosaminoglycan Synthesis. FEBS Letters, 334, 121-124. http://dx.doi.org/10.1016/0014-5793(93)81695-V
[17]	Ceperuelo-Mallafre, V., Miranda, M., Chacon, M.R., Vilarrasa, N., Megia, A., Gutierrez, C., Fernandez-Real, J.M., Gomez, J.M., Caubet, E., Frühbeck, G. and Vendrell, J. (2007) Adipose Tissue Expression of the Glycerol Channel Aquaporin-7 Gene Is Altered in Severe Obesity but Not in Type 2 Diabetes. Journal of Clinical Endocrinology & Metabolism, 92, 3640-3645. http://dx.doi.org/10.1210/jc.2007-0531
[18]	Prudente, S., Flex, E., Morini, E., Turchi, F., Capponi, D., De Cosmo, S., Tassi, V., Guida, V., Avogaro, A., Folli, F., Maiami, F., Frittitta, L., Dallapiccola, B. and Trischitta, V. (2007) A Functional Variant of the Adipocyte Glycerol Channel Aquaporin 7 Gene Is Associated With Obesity and Related Metabolic Abnormalities. Diabetes, 56, 1468-1474. http://dx.doi.org/10.2337/db06-1389
[19]	Xu, C., He, J., Jiang, H., Zu, L., Zhai, W., Pu, S. and G. Xu, (2009) Direct Effect of Glucocorticoids on Lipolysis in Adipocytes. Molecular Endocrinology, 23, 1161-1170. http://dx.doi.org/10.1210/me.2008-0464
[20]	Peckett, A.J., Wright, D.C. and Riddell, M.C. (2011) The Effects of Glucocorticoids on Adipose Tissue Lipid Metabolism. Metabolism Clinical and Experimental, 60, 1500-1510. http://dx.doi.org/10.1016/j. metabol.2011.06.012
[21]	Campbell, J.E., Peckett, A.J., D’souza, A.M., Hawke, T.J. and Riddell, M.C. (2011) Adipogenic and Lipolytic Effects of Chronic Glucocorticoid Exposure. American Journal of Physiology-Cell Physiology, 300, C198-C209. http://dx.doi.org/10.1152/ajpcell.00045.2010
[22]	Kaya, G. and Saurat, J.H. (2007) Dermatoporosis: A Chronic Cutaneous Insufficiency/Fragility Syndrome. Clinicopathological Features, Mechanisms, Preventation and Potential Treatments. Dermatology, 215, 284-294. http://dx.doi.org/10.1159/000107621
[23]	Schoepe, S., Schacke, H., May, E. and Asadullah, K. (2006) Glucocorticoid Therapy-Induced Skin Atrophy. Experimental Dermatology, 15, 406-420. http://dx.doi.org/10.1111/j.0906-6705.2006.004 35.x
[24]	Kaya, G., Tran, C., Sorg, O., Hotz, R., Grand, D., Carraux, P., Didierjean, L., Stamenkovic, I. and Saurat, J.-H. (2006) Hyaluronate Fragments Reserve Skin Atrophy by CD44-Dependent Mechanism. PLoS Medicine, 3, e493. http://dx.doi.org/10.1371/journal.pmed.0030493
[25]	Zhang, W., Watson, C.E., Liu, C., Williams, K.J. and Werth, V.P. (2000) Glucocorticoids Induce a Near-Total Suppression of Hyaluronan Synthase mRNA in Dermal Fibroblasts and in Osteoblasts: A Molecular Mechanism Contributing to Organ Atrophy. Biochemical Journal, 349, 91-97. http://dx.doi.org/10.1042/0264-6021:3490091
[26]	Gebhardt, C., Averbeck, M., Diedenhofen, N., Willenberg, A., Anderegg, U., Sleeman, J.P. and Simon, J. (2010) Dermal Hyaluronan Is Rapidly Reduced by Topical Treatment with Glucocorticoids. Journal of Investigative Dermatology, 130, 141-149. http://dx.doi.org/10.1038/jid.2009.210
[27]	Tchoukalova, Y.D., Koutsari, C., Karpyak, M.V., Votruba, S.B., Wendland, E. and Jensen, M.D. (2008) Subcutaneous Adipocytes Size and Body Fat Distribution. American Journal of Clinical Nutrition, 87, 56-63.
[28]	Wilkinson, T.S., Potter-Perigo, S., Tsoi, C., Altman, L.C. and Wight, T.N. (2004) Pro- and Anti-Inflammatory Factors Cooperate to Control Hyaluronan Synthesis in Lung Fibroblasts. American Journal of Respiratory Cell and Molecular Biology, 31, 92-99. http://dx.doi.org/10.1165/rcmb.2003-0380OC
[29]	Stern, R., Asari, A.A. and Sugahara, K.N. (2006) Hyaluronan Fragments: An Information-Rich System. European Journal of Cell Biology, 85, 699-715. http://dx.doi.org/10.1016/j.ejcb.2006.05.009
[30]	Divoux, A., Tordjman, J., Lacasa, D., Veyrie, N., Hugol, D., Aissat, A., Basdevant, A., Guerre-Millo, M., Poitou, C., Zucker, J.-D., Bedossa, P. and Clement, K. (2010) Fibrosis in Human Adipose Tissue: Composition, Distribution, and Link with Lipid Metabolism and Fat Mass Loss. Diabetes, 59, 2817-2825. http://dx.doi.org/10.2337/db10-0585
[31]	Cinti, S., Mitchell, G., Barbatelli, G., Murano, I., Ceresi, E., Faloia, E., Wang, S., Fortier, M., Greenberg, A.S. and Obin, M.S. (2005) Adipocyte Death Defines Macrophage Localization and Function in Adipose Tissue to Obese Mice and Humans. Journal of Lipid Research, 46, 2347-2355. http://dx.doi.org/10.1194/jlr.M500294-JLR200
[32]	Pajvani, U.B., Trujillo, M.E., Combs, T.P., Iyengar, P., Jelicks, L., Roth, K.A., Kitsis, R.N. and Scherer, P.E. (2005) Fat Apoptosis through Targeted Activation of Caspase 8: A New Mouse Model of Inducible and Reversible Lipoatrophy. Nature Medicine, 11, 797-803. http://dx.doi.org/10.1038/ nm1262
[33]	Sun, K., Kusminski, C.M. and Scherer, P.E. (2011) Adipose Tissue Remodelling and Obesity. The Journal of Clinical Investigations, 121, 2094-2101. http://dx.doi.org/10.1172/JCI45887
[34]	Franco, W., Kothare, A., Ronan, S.J., Grekin, R.C. and McCalmont, T.H. (2010) Hyperthermic Injury to Adipocyte Cells by Selective Heating of Subcutaneous Fat with a Novel Radiofrequency Device: Feasibility Studies. Lasers in Surgery and Medicine, 42, 361-370. http://dx.doi.org/10.1002/lsm. 20925
[35]	Vitali, A., Murano, I., Zingaretti, M.C., Frontini, A., Ricquier, D. and Cinti, S. (2012) The Adipose Organ of Obesity-Prone C57BL/6J Mice Is Composed of Mixed White and Brown Adipocytes. Journal of Lipid Research, 53, 619-629. http://dx.doi.org/10.1194/jlr.M018846
[36]	Harms, M. and Seale, P. (2013) Brown and Beige Fat: Development, Function and Therapeutic Potential. Nature Medicine, 19, 1252-1263. http://dx.doi.org/10.1038/nm.3361
[37]	Bonet, M.L., Oliver, P. and Palou, A. (2012) Pharmacological and Nutritional Agents Promoting Browning of White Adipose Tissue. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1831, 969-985. http://dx.doi.org/10.1016/j.bbalip.2012.12.002
[38]	Kruglikov, I.L. (2013) Is the Depletion of Hyaluronan in Hypertrophic Fat Tissue a Key Event in Body-Contouring Procedures? The American Journal of Cosmetic Surgery, 30, 244-245.
[39]	Kruglikov, I.L. (2013) Kontroversen in der ?sthetischen Medizin. 7. Kryolipolyse—Apoptose vs. Thermogenese. Kosmetische Medizin, 34, 202-207.