Acute and Sub-Acute Toxicity of Ethanolic Leaf Extracts of Rumex abyssinica Jacq. (Polygonaceae) and Mentha spicata L. (Lamiaceae)

DOI: 10.4236/pp.2014.53038   PDF   HTML     4,395 Downloads   6,469 Views   Citations


Rumex abyssinica Jacq. (Polygonaceae) is locally used in management of allergies and female reproductive healthcare; whereas Mentha spicata L. (Lamiaceae) is used to treat gastrointestinal and respiratory distress, dandruff, halitosis and malaria in Uganda. Owing to the paucity of data on their safety profiles, this study evaluated the acute and sub-acute toxicities of 70% ethanolic leaf extracts of both plants in mice and Wistar albino rats. The oral acute toxicity of both plants was evaluated in Swiss mice of 7-8 weeks old (16-22 g) body weight and LD50 determined. Sub-acute toxicity was evaluated in Wistar albino rats (6 per group) at dose rates of 500, 1000 and 1500 mg/kg for 28 days. The LD50 of R. abyssinica and M. spicata in mice was 7727 mg/kg and 13,606 mg/kg body weight, respectively. General signs of toxicity due to large doses of both plants extract included hyperurination, abdominal muscle twitches and convulsions. In the sub-acute toxicity test, rats treated with both extracts did not exhibit any clinical signs of toxicity; no mortality and changes in body weight were observed. R. abyssinica did not cause significant changes in haematological indices, except a significant increase in HCT (p < 0.05). However, a dose dependant significant decrease in HCT (p < 0.05) and a significant increase (p < 0.05) in the levels of WBC, LMY and MCHC were observed in rats treated with M. spicata extract. Biochemical test showed that both extracts caused a significant dose dependent increase (p < 0.05) in levels of ALT and ALP. Marked increase in the levels of AST was also observed in rats treated with M. spicata extract. Of the two extracts, only rats treated with

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Mugisha, M. , Ndukui, J. , Namutembi, A. , Waako, P. , Karlson, A. and Vudriko, P. (2014) Acute and Sub-Acute Toxicity of Ethanolic Leaf Extracts of Rumex abyssinica Jacq. (Polygonaceae) and Mentha spicata L. (Lamiaceae). Pharmacology & Pharmacy, 5, 309-318. doi: 10.4236/pp.2014.53038.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Daswani, G.P., Brijesh, S. and Birdi, J.T. (2006) Preclinical Testing of Medicinal Plants: Advantages and Approaches. Workshop Proceedings on Approaches towards Evaluation of Medicinal Plants Prior to Clinical Trial, Foundation for Medical Research at Yashwantrao Chavan Academy of Development Administration (YASHADA), Pune, 60-77.
[2] Ogbonnia, S., Adekunle, A.A., Bosa, M.K. and Enwuru, V.N. (2008) Evaluation of Acute and Sub-Acute Toxicity of Alstonia congensis Engler (Apocynaceae) Bark and Xylopia aethiopica (Dunal) A. Rich (Annonaceae) Fruits Mixtures Used in the Treatment of Diabetes. African Journal of Biotechnology, 7, 701-705.
[3] Rahma, A. and Choudhary, M.I. (1999) Recent Studies on Bioactive Natural Products. Pure and Applied Chemistry, 16, 1079-1081.
[4] Bnouham, M., Merhfour, F.Z. and Elachoui, M. (2006) Toxic Effects of Some Medicinal Plants Used in Moroccan Traditional Medicine. Moroccan Journal of Biology, 2, 21-30.
[5] Inamul, H. (2004) Safety of Medicinal Plants. Pakistan Journal of Medical Research, 43, 4.
[6] Vanherweghem, J.L., Depierreux, M. and Tielemans, C. (1993) Rapidly Progressive Interstitial Renal Fibrosis in Young Women: Association with Slimming Regimen Including Chinese Herbs. Lancet, 341, 387-391.
[7] World Health Organization (WHO) (2004) WHO Guidelines on Safety Monitoring of Herbal Medicines in Pharmacovigilance Systems.
[8] Cooper, E.L. (2004) Complementary and Alternative Medicine, When Rigorous, Can Be Science. Evidence-Based Complementary and Alternative Medicine, 1, 1-4.
[9] Suzuki, N. (2004) Complementary and Alternative Medicine: A Japanese Perspective. Evidence-Based Complementary and Alternative Medicine, 1, 113-118.
[10] Adjanohoun, J.F., Aboubakar, N., Dramane, K., Ebot, M.E., Ekpere, J.A., Enow-Orock, E.G., Focho, D., Gbile, Z.O., Kamanyi, A., Kamsu Kom, J., Keita, A., Mbenkum, T., Mbi, C.N., Nkongmeneck, B., Satabie, B., Sofowora, A., Tamze, V. and Wirmum, C.K. (1996) Traditional Medicine and Pharmacopoeia: Contribution to Ethno Botanical and Floristic Studies in Cameroon. Organization of African Unity; Scientific, Technical and Research Commission, Centre Nationale de Production des Manuels Scolaires, Porto-Novo, Benin, 207-209.
[11] Organization for Economic Co-operation and Development (OECD) (2001) The OECD Guideline for Testing of Chemical. The Organization of Economic Co-Operation Development, Paris, 1-14.
[12] OECD (2008) Repeated Dose Oral Toxicity Test Method. OECD Guidelines for Testing of Chemicals. Organization for Economic Cooperation and Development, Paris, 327.
[13] Raza, M., Al-Shabanah, O.A., El-hadiyah, T.M. and Al-Majed, A.A. (2002) Effect of Prolonged Vigabatrin Treatment on Hematological and Biochemical Parameters in Plasma, Liver and Kidney of Swiss Albino Mice. Scientia Pharmaceutica, 70, 135-145.
[14] Gosh, M.N. (1996) Fundamentals of Experimental Pharmacology. 4th Edition, Scientific Book Agency Calcutta, Calcutta.
[15] Cheesebrough, M. (1991) Medical Laboratory Manual for Tropical Countries. 2nd Edition, ELBS, 1, 605.
[16] Feldman, B.F., Zinkl, J.G. and Jain, N.C. (2000) Schalm’s Veterinary Haematology. 5th Edition, Williams & Wilkins, Lippincott.
[17] Sacher, R.A. and McPherson, R.A. (1991) Widmann’s Clinical Interpretation of Laboratory Tests. 10th Edition, Philadelphia, 1-6.
[18] Guyton, A.C. and Hall, J. (2000) Textbook of Medical Physiology. 10th Edition, Harcourt International Edition, W.B Saunder Company, Philadelphia, 279-281.
[19] Hiromich, M.I. (2001) Saponins in Garlic as Modifiers of the Risk of the Cardiovascular Disease. Journal of Nutrition, 131, 1000S-1005S.

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