Induced Accumulation of Polyphenolics and Flavonoids in Cyanobacteria under Salt Stress Protects Organisms through Enhanced Antioxidant Activity


Induced accumulation of polyphenolics and flavonoids in cyanobacterial strains grown under different salt concentrations is correlated with their growth under stress conditions and enhanced antioxidant activity. Plectonema boryanum, Hapalosiphon intricatus, Anabaena doliolum and Oscillatoria acuta grown for 21 days under different salt concentrations (80, 160, 240, 320 and 400 mM) in BG11 medium showed differential growth responses in terms of biomass, total protein, chlorophyll content, total content of polyphenol, flavonoid and carotenoid, accumulation of phenolic acids (gallic, caffeic, chlorogenic, ferullic and vanillic) and flavonoids (rutin and quercetin). Cyanobacterial extracts showed prominent free radical scavenging antioxidant activity (AOA) in terms of % DPPH discoloration. Highly significant (p < 0.05) and strong correlation was found between TPC and AOA (r = 0.974). Other positive but non-significant (p < 0.05) correlations were observed between AOA and gallic acid (r = 0.893) and AOA and caffeic acid (r = 0.931). Significant and strong correlation was also observed between gallic and caffeic acid (r = 0.973). Positive but lesser magnitude correlations were recorded between TPC and caffeic acid (r = 0.905), TPC and gallic acid (r = 0.920), gallic and vanillic acid (r = 0.916) and caffeic and vanillic acid (r = 0.814). An integrated combination of growth parameters, salt-induced accumulation of phenylpropanoids and stress-derived subsequent antioxidant property of cyanobacterial extracts is thought to provide evidence that secondary metabolic changes can act as the possible alternative mechanism to overcome stress-induced damages in cells.

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Singh, D. , Prabha, R. , Meena, K. , Sharma, L. and Sharma, A. (2014) Induced Accumulation of Polyphenolics and Flavonoids in Cyanobacteria under Salt Stress Protects Organisms through Enhanced Antioxidant Activity. American Journal of Plant Sciences, 5, 726-735. doi: 10.4236/ajps.2014.55087.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] López-Rodas, V., Maneiro, E. and Costas, E. (2006) Adaptation of Cyanobacteria and Microalgae to Extreme Environmental Changes Derived from Anthropogenic Pollution. Limnetica, 25, 403-410.
[2] Pandhal, J., Wright, P.C. and Biggs, C.A. (2008) Proteomics with a Pinch of Salt: A Cyanobacterial Perspective. Saline Systems, 4, 1-18.
[3] Mikkat, S., Hagemann, M. and Schoor, A. (1996) Active Transport of Glucosylglycerol Is Involved in Salt Adaptation of the Cyanobacterium Synechocystis sp. Strain PCC 6803. Microbiology, 142, 1725-1732.
[4] Allakhverdiev, S.I., Klimov, V.V. and Hagemann, M. (2005) Cellular Energization Protects the Photosynthetic Machinery against Salt-Induced Inactivation in Synechococcus. BBA-Bioenergetics, 1708, 201-208.
[5] La Camera, S., Gouzerh, G., Dhondt, S., Hoffman, L., Frittig, B., Legrand, M. and Heitz, T. (2004) Metabolic Reprogramming in Plant Innate Immunity: The Contributions of Phenylpropanoid and Oxylipin Pathways. Immunological Reviews, 198, 267-284.
[6] Janas, K.M., Cvikrova, M., Palagiewicz, A. and Eder, J. (2000) Alterations in Phenylpropanoid Content in Soybean Roots during Low Temperature Acclimation. Plant Physiology and Biochemistry, 38, 587-593.
[7] Singh, U.P., Sarma, B.K. and Singh, D.P. (2003) Effect of Plant Growth Promoting Rhizobacteria and Culture Filtrate of Sclerotium rolfsii on Phenolic and Salicylic Acid Contents in Chickpea (Cicer arietinum). Current Microbiology, 46, 131-140.
[8] Vogt, T. (2010) Phenylpropanoid Biosynthesis. Molecular Plant, 3, 2-20.
[9] Oueslati, S., Karray-Bouraoui, N., Attia, H., Rabhi, M., Ksouri, R. and Lachaal, M. (2010) Physiological and Antioxidant Responses of Mentha pulegium (Pennyroyal) to Salt Stress. Acta Physiologiae Plantarum, 32, 289-296.
[10] Edreva, A., Velikova, V., Tsonev, T., Dagnon, S., Gürel, A., AktaS, L. and Gesheva, E. (2008) Stress-Protective Role of Secondary Metabolites: Diversity of Functions and Mechanisms. General and Applied Plant Physiology, XXXIV, 67-78.
[11] Ramakrishna, A. and Ravi Shankar, G.A. (2011) Influence of Abiotic Stress Signals on Secondary Metabolites in Plants. Plant Signal Behaviour, 6, 1720-1731.
[12] Agati, G., Biricolti, S., Guidi, L., Ferrini, F., Fini, A. and Tattini, M. (2011) The Biosynthesis of Flavonoids Is Enhanced Similarly by UV Radiation and Root Zone Salinity in L. vulgare Leaves. Journal of Plant Physiology, 168, 204-212.
[13] Korkina, L.G. (2007) Phenylpropanoids as Naturally Occurring Antioxidants from Plant Defence to Human Health. Cellular and Molecular Biology, 53, 15-25.
[14] Ferjani, A., Mustardy, L., Sulpice, R., Marin, K., Suzuki I., Hageman, M. and Murata, N. (2003) Glucosylglycerol, a Compatible Solute, Sustains Cell Division under Salt Stress. Plant Physiology, 131, 1628-1637.
[15] Kim, D.O., Jeong, S.W. and Lee, C.Y., (2003) Antioxidant Capacity of Phenolic Phytochemicals from Various Cultivars of Plums. Food Chemistry, 81, 321-326.
[16] Beta, T., Nam, S., Dexter, J.E. and Sapirstein, H.D. (2005) Phenolic Content and Antioxidant Activity of Pearled Wheat and Roller Milled Fractions. Cereal Chemistry, 82, 390-393.
[17] Kesheri, M., Richa and Sinha, R.P. (2011) Antioxidants as Natural Arsenal against Multiple Stresses in Cyanobacteria. International Journal of Pharma and Bio Sciences, 2, B169-B187.
[18] Kanesaki, Y., Suzuki, I., Allakhverdiev, S.I., Mikami, K. and Murata, N. (2002) Salt Stress and Hyperosmotic Stress Regulate the Expression of Different Sets of Genes in Synechocystis sp. PCC 6803. Biochemica Biophysica Research Communication, 290, 339-348.
[19] Tang, D., Shi, S., Li, D., Hu, C. and Liu, Y. (2007) Physiological and Biochemical Responses of Scytonema javanicum (cyanobacterium) to Salt Stress. Journal of Arid Environments, 71, 312-320.
[20] Allakhverdiev, S.I. and Murata, N. (2008) Salt Stress Inhibits Photosystems II and I in Cyanobacteria. Photosynthetic Research, 98, 529-39.
[21] Hagemann, M. (2011) Molecular Biology of Cyanobacterial Salt Acclimation. FEMS Microbiology Review, 35, 87-123.
[22] Mpofu, A., Sapirstein, H.D. and Beta, T. (2006) Genotype and Environmental Variation in Phenolic Content, Phenolic Acid Composition, and Antioxidant Activity of Hard Spring Wheat. Journal of Agricultural and Food Chemistry, 54, 1265-1270.
[23] Karamac, M., KosiÑska, A. and Pegg, R.B. (2005) Comparison of Radical-Scavenging Activities for Selected Phenolic Acids. Polish Journal of Food Nutrition Science, 14, 165-170.
[24] Abogadallah, G.A. (2010) Antioxidative Defense under Salt Stress. Plant Signaling & Behavior, 5, 369-374.

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