Analysis of Genetic Diversity among Wild and Cultivated Chickpea Genotypes Employing ISSR and RAPD Markers

Pramod Kumar Singh, Himanshu Sharma, Nidhi Srivastava, Sameer S. Bhagyawant

Department of Biotechnology, Banasthali Univer-sity, Banasthali, India.
Institute of Himalayan Bioresource Technology Palampur, Palampur, India.
School of Studies in Biotechnology, Jiwaji Univer-sity, Gwalior, India.

DOI: 10.4236/ajps.2014.55082                

Abstract

Chickpea (Cicer airetinum L.) is an important and most preferred food legume in many parts of the world especially in the Indian sub-continent. Molecular analysis of chickpea using DNA technology has been carried out to identify the diverse genetic base of the cultivars for selected preferential introductions as efficient edible elements. A few of these genetic stocks have been documented here to tap their genetic diversity. The status shows that the level of polymorphism in this species is low. Using PCR based markers, marker assisted selection of polymorphy is one of the established techniques. Here, this procedure has been employed to expedite gene/QTL pyramiding in the chickpea. The study presented here includes analysis of 12 germplasms of chickpea. Standard CTAB method has been performed, with certain modifications, to get better intensity and resolution of DNA bands. Extracted DNA, amplified with 41 RAPD and 21 ISSR primers are thereby tested to determine genetic diversity. The presentation discusses the results of chickpea germplasm diversity on the basis of these observations.

Keywords

Chickpea; Randomly Amplified Polymorphic DNA and Inter Simple Sequence Repeats; Genetic Diversity

Conflicts of Interest

The authors declare no conflicts of interest.

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