Effectiveness of Ringworm Cassia and Turmeric Plant Extracts on Growth Inhibition against Some Important Plant Pathogenic Fungi

Porntip Wongkaew, Wantana Sinsiri

Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Mahasarakham, Thailand.
Plant pathology Division, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand.

DOI: 10.4236/ajps.2014.55076                

Abstract

Crude plant extracts of ringworm cassia, Cassia alata L. and turmeric, Curcuma longa L. were prepared by either hot water or organic solvents such as ethanol and ether. Various concentrations of the crude extract were then subjected to an in vitro test for their effectiveness on mycelia growth inhibition against some important plant pathogenic fungi such as Alternaria alternata, Colletotrichum gloeosporioides, Fusarium oxysporum fsp. lycopersici, Sclerotium rolfsii, Phytophthora infestans and Pythium sp. in comparison to commercial fungicides such as copper oxychloride and mancozeb. Reduction of the fungal growth was significantly obtained with C. longa extracts and the best median effective inhibitory concentration (IC50) value of 6.07, 6.50 and 7.13 mg/ml was from the ethanol extract for S. rolfsii, C. gloeosporioides and F. oxysporum fsp. lycopersici respectively. While all extracts from C. alata were almost the least effective against these fungi. The efficacy of C. longa extracts therefore, provided an alternative regime for the control of the fungal diseases and a promising appreciable choice for a replacement of chemical fungicides.

Keywords

Crude Plant Extracts; Cassia alata; Curcuma longa; Commercial Fungicides; Plant Pathogenic Fungi; Commercial Fungicides; Mycelia Growth Inhibition

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Agrios, G.N. (2005) Plant Pathology. 5th Edition, Elsevier Academic Press, New York.
[2]	Bunyaprapatr, N. and Chokchaichareonporn, O. (1996) Indigenous Medicinal Plants. Medicinal Plants Information Office, Mahidol University Press, Bangkok.
[3]	Markinde, A.A., Igoli, J.O., Amal, L.T.A., Shaibu, S.J. and Garbal, A (2007) Antimicrobial Activity of Cassia alata. African Journal of Biotechnology, 6, 1509-1510.
[4]	Akram, M., Uddini, S., Ahmed, A., Usmanghani, K., Hannan, A., Mohiuddin, E. and Asif, M. (2010) Curcuma longa and Curcumin: A Review article. Romanian Journal of Biology and Plant Biology, 55, 65-70.
[5]	Collazo, C., Chacon, O. and Barras, O. (2006) Programmed cell death in plants and animals. Biotecnología Aplicada, 23, 1-10.
[6]	Barnett, H.L. and Hunter, B.B. (1986) Illustrated Genera of Imperfect Fungi, 4th Edition. Macmillan Publishing Co., New York.
[7]	Owoyale, J.A., Olatunji, G.A. and Oguntoye, S.O. (2005) Antifungal and Antibacterial Activities of an Alcoholic Extract of Senna alata Leaves. Journal of Applied Science and Environment, 9, 105-107.
[8]	Sule, W.F., Okonko, I.O., Joseph, T.A., Ojezele, M.O., Nwanze, J.C., Alli, J.A., Adewale, O.G. and Ojezele, O.J. (2010) In-Vitro Antifungal Activity of Senna Alata Linn. Crude Leaf Extract. Research Journal of Biological Sciences, 5, 275-284. http://dx.doi.org/10.3923/rjbsci.2010.275.284
[9]	Phongpaichit, S., Pujenjob, N., Rukachaisirikul, V. and Ongsakul, M. (2004) Antifungal Activity from Leaf Extracts of Cassia alata L., Cassia fistula L. and Caaaia tora L. Songklanakarin Journal of Science and technology, 26, 741-748.
[10]	Doughari, J.H. and Okafor, B. (2007) Antimicrobial Activity of Senna alata Linn.. East and Central African Journal of Pharmaceutical Sciences, 10, 17-21.
[11]	Timothy, M.S.Y., Wazis, C.H., Adati, R.G. and Maspalma, I.D. (2012) Antifungal Activity of Aqueous and Ethanolic Leaf Extracts of Cassia alata Linn. Journal of Applied Pharmaceutical Science, 2, 182-185.
[12]	Abubacker, M.N., Ramanathan, R. and Kumar, T.S. (2008) In Vitro Antifungal Activity of Cassia alata Linn. Flower Extract. Natural Product Radiance, 7, 6-9.
[13]	Gupta, D.S., Jann, B., Bajpai, K.S. and Sharma, S.C. (1987) Structure of Galactomannan from Cassia alata Seed. Carbohydrate Research, 162, 271-276. http://dx.doi.org/10.1016/0008-6215(87)80222-7
[14]	Villasenor, I.M., Canlas, A.P., Pascua, M.P.I., Sabando, M.N. and Soliven, L.A.P. (2002) Bioactivity Studies on Cassia alata Linn. Leaf Extract. Phytotherapy Researches, 16, S93-S96. http://dx.doi.org/10.1002/ptr.768
[15]	Singh, M R. P. and Jain, D.A. (2011) Evaluation of Antimicrobial Activity of Volatile Oil and Total Curcuminoids Extracted fromTurmeric. International Journal of ChemTech Research, 3, 1172-1178.
[16]	Harit, J., Barapatre, A., Prajapati, M., Aadil, K. R. and Senapati, S. (2013) Antimicrobial Activity of Rhizome of Selected Curcuma Variety. International Journal of Life Sciences Biotechnology and Pharma Research, 2, 183-189.
[17]	Sawant, R.S. and Godghate, A.G. (2013) Qualitative Phytochemical Screening of Rhizomes of Cucurma longa Linn. International Journal of Science, Environment and Technology, 2, 634-641.
[18]	Ungphaiboon, S., Supavita, T., Singchangchai, P., Sungkarak, S., Rattanasuwan, P. and Itharat, A. (2005) Study on Antioxidant and Antimicrobial Activities of Turmeric Clear Liquid Soap for Wound Treatment of HIV Patients. Songklanakarin Journal of Science and Technology, 27, 569-578.
[19]	Lawhavinit, O., Kongkathip, N. and Kongkathip, B. (2010) Antimicrobial Activity of Curcuminoids from Curcuma longa L. on Pathogenic Bacteria of Shrimp and Chicken. Kasetsart Journal (Natural Science), 44, 364-371.
[20]	Saleem, M., Daniel, B. and Murli, K. (2011) Antimicrobial Activity of three Different Rhizomes of Curcuma longa & Curcuma aromatic on Uropathogens of Diabetic Patients. International Journal of Pharmacy and Pharmaceutical Sciences, 3, 273-279.
[21]	Jamal, Q., Munir, S., Sherwani, S.K., Sualeh, M., Jabeen, U., Malik, M. and Hussain, M. (2013) Antibacterial Activity of Two Medicinal Plants: Withania somnifera and Curcuma longa. European Academic Research, 1, 1335-1345.
[22]	Alavijeh, P. K., Alavijeh, P.K. and Sharma, D. (2012) A Study of Antimicrobial Activity of Few Medicinal Herbs. Asian Journal of Plant Science and Research, 2, 496-502.
[23]	Niamsa, N. and Sittiwet, C. (2009) Antimicrobial Activity of Curcuma longa Aqueous Extract. Journal of Pharmacology and Toxicology, 4, 173-77. http://dx.doi.org/10.3923/jpt.2009.173.177
[24]	Hegde, M.N., Shetty, S., Yelapure, M. and Patil, A. (2012) Evaluation of Antimicrobial Activity of Aqueous and Hydro-Alcoholic Curcuma Longa Extracts against Endodontic Pathogens. IOSR Journal of Pharmacy, 2, 192-198. http://dx.doi.org/10.9790/3013-0220192198
[25]	Kumar, A., Bhatii, V., Kumar, A., Patil, S., Bhatia, V. and Kumar, A. (2011) Screening of Various Plant Extracts for Antifungal Activity against Candida Species. World Journal of Science and Technology, 1, 2231-2587.