GenoType MTBDRplus as a Complementary Tool for the Typing of Mycobacterium tuberculosis

Timothée Ouassa, Guillaume Yao Loukou, Hortense Faye-Kette

Department of Bacteriology and Virology, Faculty of Medical Sciences, University Félix Houphou?t-Boigny, Abidjan, C?te d’Ivoire;Institut Pasteur de C?te d’Ivoire, Abidjan, C?te d’Ivoire.
Department of Bacteriology and Virology, Faculty of Pharmaceutical and Biological Sciences, University Félix Houphou?t-Boigny, Abidjan, C?te d’Ivoire;Centre for Diagnostic and Research on AIDS and Opportunistic Infections, Teaching Hospital of Treichville, Abidjan, C?te d’Ivoire.
Department of Bacteriology and Virology, Faculty of Pharmaceutical and Biological Sciences, University Félix Houphou?t-Boigny, Abidjan, C?te d’Ivoire;National Laboratory for Public Health, Abidjan, C?te d’Ivoire.

DOI: 10.4236/aid.2014.41005                

Abstract

The aim of this study was to investigate the usefulness of combining profiles obtained by using a line probe assay (LPA) originally intended to characterize the resistance of two major anti-tuberculosis drugs to the association of spoligotyping and MIRU-VNTR, in order to improve its discriminatory power. For this purpose, 74 strains of Mycobacterium tuberculosis belonging to the same cluster after spoligotyping were further typed by using the 24 loci MIRU/VNTR. These strains were then tested by the GenoType MTBDRplus, and profiles obtained were analyzed within previously obtained clusters. The combination of spoligotying and MIRU-VNTR led to the consolidation of 56 of them (75.7%) in 9 clusters. Most of the strains (54, 96.4%) were multidrug resistant (MDR). From the 9 initial clusters, the addition of GenoType MTBDRplus helped to define 26 profiles including 11 unique profiles, and 3 original clusters remained undifferentiated. Results obtained express the relevance of combining this method which improved quite significantly the discriminatory power in typing Mycobacterium tuberculosis.

Keywords

Mycobacterium tuberculosis; MIRU-VNTR; Spoligotyping; GenoType MTBDRplus; Côte d’Ivoire

Conflicts of Interest

The authors declare no conflicts of interest.

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