GenoType MTBDRplus as a Complementary Tool for the Typing of Mycobacterium tuberculosis
Timothée Ouassa, Guillaume Yao Loukou, Hortense Faye-Kette
Department of Bacteriology and Virology, Faculty of Medical Sciences, University Félix Houphou?t-Boigny, Abidjan, C?te d’Ivoire;Institut Pasteur de C?te d’Ivoire, Abidjan, C?te d’Ivoire.
Department of Bacteriology and Virology, Faculty of Pharmaceutical and Biological Sciences, University Félix Houphou?t-Boigny, Abidjan, C?te d’Ivoire;Centre for Diagnostic and Research on AIDS and Opportunistic Infections, Teaching Hospital of Treichville, Abidjan, C?te d’Ivoire.
Department of Bacteriology and Virology, Faculty of Pharmaceutical and Biological Sciences, University Félix Houphou?t-Boigny, Abidjan, C?te d’Ivoire;National Laboratory for Public Health, Abidjan, C?te d’Ivoire.
DOI: 10.4236/aid.2014.41005   PDF    HTML     3,517 Downloads   5,452 Views   Citations


The aim of this study was to investigate the usefulness of combining profiles obtained by using a line probe assay (LPA) originally intended to characterize the resistance of two major anti-tuberculosis drugs to the association of spoligotyping and MIRU-VNTR, in order to improve its discriminatory power. For this purpose, 74 strains of Mycobacterium tuberculosis belonging to the same cluster after spoligotyping were further typed by using the 24 loci MIRU/VNTR. These strains were then tested by the GenoType MTBDRplus, and profiles obtained were analyzed within previously obtained clusters. The combination of spoligotying and MIRU-VNTR led to the consolidation of 56 of them (75.7%) in 9 clusters. Most of the strains (54, 96.4%) were multidrug resistant (MDR). From the 9 initial clusters, the addition of GenoType MTBDRplus helped to define 26 profiles including 11 unique profiles, and 3 original clusters remained undifferentiated. Results obtained express the relevance of combining this method which improved quite significantly the discriminatory power in typing Mycobacterium tuberculosis.

Share and Cite:

Ouassa, T. , Loukou, G. and Faye-Kette, H. (2014) GenoType MTBDRplus as a Complementary Tool for the Typing of Mycobacterium tuberculosis. Advances in Infectious Diseases, 4, 26-29. doi: 10.4236/aid.2014.41005.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Schurch, A.C. and van Soolingen, D. (2012) DNA Fingerprinting of Mycobacterium Tuberculosis: From Phage Typing to Whole-Genome Sequencing. Infection, Genetics and Evolution, 12, 602-609.
[2] Gardy, J.L., Johnston, J.C., Ho Sui, S.J., Cook, V.J., Shah, L., Brodkin, E., Rempel, S., Moore, R., Zhao, Y., Holt, R., et al. (2011) Whole-Genome Sequencing and Social-Network Analysis of a Tuberculosis Outbreak. The New England Journal of Medicine, 364, 730-739.
[3] Roetzer, A., Diel, R., Kohl, T.A., Ruckert, C., Nubel, U., Blom, J., Wirth, T., Jaenicke, S., Schuback, S., Rusch-Gerdes, S., et al. (2013) Whole Genome Sequencing versus Traditional Genotyping for Investigation of a Mycobacterium Tuberculosis Outbreak: A Longitudinal Molecular Epidemiological Study. PLoS Medicine, 10, Article ID: e1001387.
[4] WHO (2008) Molecular Line Probe Assays for Rapid Screening of Patients at Risk of Multi-Drug Resistant Tuberculosis (MDR-TB). World Health Organization, Geneva.
[5] Ouassa, T., Borroni, E., Loukou, G.Y., Faye-Kette, H., Kouakou, J., Menan, H. and Cirillo, D.M. (2012) High Prevalence of Shared International Type 53 among Mycobacterium Tuberculosis Complex Strains in Retreated Patients from Côte d’Ivoire. PloS One, 7, Article ID: e45363.
[6] Hunter, P.R. and Gaston, M.A. (1988) Numerical Index of the Discriminatory Ability of Typing Systems: An Application of Simpson’s Index of Diversity. Journal of Clinical Microbiology, 26, 2465-2466.
[7] Torok, M.E., Reuter, S., Bryant, J., Koser, C.U., Stinchcombe, S.V., Nazareth, B., Ellington, M.J., Bentley, S.D., Smith, G.P., Parkhill, J., et al. (2013) Rapid Whole-Genome Sequencing for Investigation of a Suspected Tuberculosis Outbreak. Journal of Clinical Microbiology, 51, 611-614.
[8] de Beer, J.L., van Ingen, J., de Vries, G., Erkens, C., Sebek, M., Mulder, A., Sloot, R., van den Brandt, A.M., Enaimi, M., Kremer, K., et al. (2013) Comparative Study of IS6110 Restriction Fragment Length Polymorphism and VariableNumber Tandem-Repeat Typing of Mycobacterium Tuberculosis Isolates in the Netherlands, Based on a 5-Year Nationwide Survey. Journal of Clinical Microbiology, 51, 1193-1198.
[9] Supply, P., Allix, C., Lesjean, S., Cardoso-Oelemann, M., Rusch-Gerdes, S., Willery, E., Savine, E., de Haas, P., van Deutekom, H., Roring, S., et al. (2006) Proposal for Standardization of Optimized Mycobacterial Interspersed Repetitive Unit-Variable-Number Tandem Repeat Typing of Mycobacterium Tuberculosis. Journal of Clinical Microbiology, 44, 4498-4510.

Copyright © 2023 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.