Reactive Arrays of Colorimetric Sensors for Metabolite and Steroid Identification

DOI: 10.4236/jst.2014.41001   PDF   HTML     3,460 Downloads   5,262 Views   Citations


The work described herein examines a rapid mix-and-measure method called DETECHIP suitable for screening of steroids and metabolites. The addition of steroids and metabolites to reactive arrays of colorimetric sensors generated characteristic color “fingerprints” that were used to identify the analyte. A color analysis tool was used to identify the analyte pool that now includes biologically relevant analytes. The mix-and-measure arrays allowed the detection of disease metabolites, orotic acid and argininosuccinic acid; and the steroids androsterone, 1,4-androstadiene, testosterone, stanozolol, and estrone. The steroid 1,4-androstadiene was also detected by this method while dissolved in synthetic urine. Some of the steroids, such as androstadiene, stanozolol, and androsterone were co-dissolved with (2-hydroxypropyl)-β-cyclodextrin in order to increase solubility in aqueous buffered solutions. The colorimetric arrays do not intend to eliminate ELISA or mass spectroscopy based screening, but to possibly provide an alternative analytical detection method for steroids and metabolites.

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Batres, G. , Jones, T. , Johnke, H. , Wilson, M. , Holmes, A. and Sikich, S. (2014) Reactive Arrays of Colorimetric Sensors for Metabolite and Steroid Identification. Journal of Sensor Technology, 4, 1-6. doi: 10.4236/jst.2014.41001.

Conflicts of Interest

The authors declare no conflicts of interest.


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