Evaluation of Lipid Peroxidation and Some Antioxidant Activities in Patients with Primary and Metastatic Liver Cancer

Ayse Arslan, Halit Demir, M. Fatih Ozbay, Harun Arslan

Department of Biochemistry, Faculty of Science, Yuzuncu Yil University, Van, Turkey.
Department of Internal Medicine, Training and Research Hospital, Van, Turkey.
Training and Research Hospital, Department of Radiology, Van, Turkey.

DOI: 10.4236/jct.2014.52024                

Abstract

Abstract: HCC is the 6th most common cancer in the world. The main risk factors associated with HCC are hepatitis B (HBV) and hepatitis C viral infections and other factors that play a role in HCC development, include aflatoxin B1 (AFB1) cigarette smoking, and chronic inflammation. The aim of this study is to investigate lipid peroxidation and some antioxidant enzyme activities in patients with primary and metastatic liver cancer. For this purpose, 25 primary and metastatic liver cancer patients and 15 healthy controls were included in the study. In blood samples taken from the patient and control groups, the main product of lipid peroxidation MDA and SOD, GSH, GPx activity levels were examined. In result of study serum MDA level is higher and erythrocyte SOD, GSH, and GPx activities were found to be significantly lower in the patient group compared with the control group (p < 0.05). As a result, liver cancer is associated with oxidative stress and antioxidant system weakens, which is an important indicator of oxidative stress, lipid peroxidation levels increased and promotes the tissue damage.

Keywords

Primary and Metastatic Liver Cancer; Lipid Peroxidation; Antioxidant Enzymes

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	J. Ferlay, H. R. Shin, F. Bray, D. Forman, C. Mather and D. M. Parkin, “Estimates of Worldwide Burden of Cancer in 2008: Globocan 2008,” International Journal of Cancer, Vol. 127, No. 12, 2010, pp. 2893-2917. http://dx.doi.org/10.1002/ijc.25516
[2]	H. B. El-Serag, “Hepatocellular Carcinoma,” New England Journal of Medicine, Vol. 365, No. 12, 2011, pp. 1118-1127. http://dx.doi.org/10.1056/NEJMra1001683
[3]	R. Mazzanti, L. Gramantieri and L. Bolondi, “Hepatocellular Carcinoma: Epidemiology and Clinical Aspects,” Molecular Aspects of Medicine, Vol. 29, No. 1-2, 2008, pp. 130-143. http://dx.doi.org/10. 1016/j.mam.2007.09.008
[4]	C. Müller, “Hepatocellular Carcinoma-Rising Incidence, Changing Therapeutic Strategies,” Wiener Medizinische Wochenschrift, Vol. 156, No. 13-14,2006, pp. 404-409. http://dx.doi.org/10.1007/ s10354-006-0316-3
[5]	N. K. Tangutur, S. F. Medvedev, F. Regenstein and L. A. Balart, “Hepatocellular Carcinoma, a Rapidly Increasing Public Health Problem: The Tulane Experience 2003-2009,” The Journal of the Louisiana State Medical Society,Vol. 163, No. 4, 2011, pp. 185-190.
[6]	B. Halliwell, “Reactive Oxygen Species in Living Systems: Source, Biochemistry and Role in Human Disease,” American Journal of Medicine, Vol. 91, No. 3C, 1991, pp.14-22. http://dx.doi.org/10.1016/ 0002-9343(91)90279-7
[7]	E. Cadenas, “Biochemistry of Oxygen Toxicity,” Annual Review of Biochemistry, Vol. 58, 1989, pp. 79-110. http://dx.doi.org/10.1146/annurev.bi.58.070189.000455
[8]	C. E. Cross, B. Halliwell, E. T. Borish, W. A. Pryor, B. N. Ames, et al., “Oxygen Radicals and Human Disease,” Annals of Internal Medicine, Vol. 107, No. 4, 1987, pp. 526-545. http://dx.doi.org/10. 7326/0003-4819-107-4-526
[9]	K. Asayama, T. Nakane, H. Uchida, H. Hayashibe, K. Dobashi and S. Nakazawa, “Serum Antioxidant Status in Streptazosin-Induced Diabetic Rat,” Hormone and Metabolic Research, Vol. 26, No. 7, 1994, pp. 617-620. http://dx.doi.org/10.1055/s-2007-1001693
[10]	R. K. Ockner, R. M. Kaikaus and N. M. Bass, “Fatty-Acid Metabolism and the Pathogenesis of Hepatocellular Carcinoma: Review and Hypothesis,” Hepatology, Vol. 18, No. 3, 1993, pp. 669-676.
[11]	C. E.Vaca, J. Wilhelm and M. Harms-Ringdal, “Interaction of Lipid Peroxidation Products with DNA. A Review,” Mutation Research, Vol. 195, No. 2, 1988, pp. 137-149. http://dx.doi.org/10.1016/0165-1110(88)90022-X
[12]	K. Ueda, S. Kobayashi, J. Morita and T. Komano, “Sitespecific DNA Damage Caused by Lipid Peroxidation Products,” Biochim Biophys Acta, Vol. 824, No. 4, 1985, pp. 341-348. http://dx.doi.org/10.1016/0167-4781(85)90041-7
[13]	C. G. Cochrane, “Cellular ?njury by Oxidants,” American Journal of Medicine, Vol. 91, No. 3C, 1991, pp. 23-30. http://dx.doi.org/10.1016/0002-9343(91)90280-B
[14]	J. Gromadizinka, W. Wasowichz, M. Andrijewski, M. Sklodowska, et al., “Glutathione and Glutathione Metabolizing Enzymes in Tissue and Blood of Breast Cancer Patients,” Neoplasma, Vol. 44, No. 1, 1997, pp. 45-51.
[15]	A. Klimczak, K. Kubiak, M. Cybulska, A. Kula, L. Dziki, et al., “Etiology of Colorectal Cancer and antioxidant Barrier of the Organism,” Pol Merkur Lekarski, Vol. 28, No. 65, 2010, pp. 223-226.
[16]	H. R. Andersen, J. B. Nielsen, F. Nielsen and P. Grandjean, “Antioxidative Enzyme Activities In Human Erithrocytes,” Clinical Chemistry, Vol. 43, No. 4, 1997, pp. 562-568.
[17]	R. Kumaraguruparan, R. Subapriya, J. Kabalimoorthy and S. Nagini, “Antioxidant Profile in the Circulation of Patients with Fibroadenoma and Adenocarcinoma of the Breast,” Clinical Biochemistry, Vol. 35, No. 4, 2002, pp. 275-279. http://dx.doi.org/10.1016/S0009-9120(02)00310-7
[18]	E. Serin, E. Yilmaz,. S. Yilmaz, E. ünsaldi and A. S. Durmus, “Ischemia-Reperfusion Injury by Oxygen Free Radicals,” Arthroplasty Arthroscopic Surgery, Vol. 9, 1998, pp. 36-39.
[19]	J. B. Mitchel and A. Russo, “The Role of Glutathione in Radiation and Drug ?nduced Cytotoxicity,” The British Journal of Cancer. Supplement, Vol. 8, 1987, pp. 96-104.
[20]	M. Y. Farooqui, W. W. Day and D. M. Zamorano, “Glutathione and Lipid Peroxidation in the Aging Rat,” Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, Vol. 88, No. 1, 1987, pp. 177-180. http://dx.doi.org/10.1016/0305-0491(87)90097-6
[21]	J. D. Hayes and L. I. Mclellan, “Glutathione and Glutathione Dependent-Enzymes Represent a Co-Ordinately Regulated Defence against Oxidative Stress,” Free Radical Research, Vol. 31, No. 4, 1999, pp. 273-300. http://dx.doi.org/10.1080/10715769900300851
[22]	C. Lindholm, A. Acheva and S. Salomaa, “Clastogenic Plasma Factors: A Short Overview,” Radiation and Environmental Biophysics, Vol. 49, No. 2, 2010. pp. 133-138. http://dx.doi.org/10.1007/s00411-009-0259-3
[23]	A. M. Jentzsch, H. Bachmann, P. Fürst and H. K. Biesalski, “Improved Analysis of Malondialdehyde in Human Body Fluids,” Free Radical Biology & Medicine, Vol. 20, No. 2, 1996, pp. 251-256. http://dx.doi.org/10.1016/0891-5849(95)02043-8
[24]	J. M. McCord and I. Fridovich, “Superoxide Dismutase: An Enzymic Function for Erythrocuprein (Hemocuprein),” Journal of Biological Chemistry, Vol. 244, No.22, 1969, pp. 6049-6055.
[25]	D. E. Paglia and W. N. Valent?ne, “Studies on the Quantitative and Qualitative Characterisation of Erytrocyte Glutahione Peroxidase,” Journal of Laboratory and Clinical Medicine, 1967, Vol. 70, No. 1, 1967, pp. 158-169.
[26]	F. Tietz, “Enzymic Method for Quantitative Determination of Nanogram Amounts of Total and Oxidized Glutathione: Applications to Mammalian Blood and Other Tissues,” Analytical Biochemistry, Vol. 27, No. 3, 1969, pp. 502-522. http://dx.doi.org/10.1016/0003-2697(69)90064-5
[27]	A. T. Diplock, “Healty Lifestyles Nutrition and Physical Activity: Antioxidant Nutrients,” ILSI Europe Concise Monograph Series, Belgium, 1998, p. 59.
[28]	R. A. Gonzalez, “Free Radicals, Oxidative Stress and DNA Metabolism in Human Cancer,” Cancer Investigation, Vol. 17, No. 5, 1999, pp. 376-377. http://dx.doi.org/10.3109/07357909909032882
[29]	L. J. Marnett, “Lipid Peroxidation-DNA Damage by Malondialdehyde,” Mutation Research, Vol. 424, No. 1-2, 1999, pp. 83-95. http://dx.doi.org/10.1016/S0027-5107(99)00010-X
[30]	C. C. Lin and M. C. Yin, “B Vitamins Deficiency and Decreased Anti-Oxidative State in Patients with Liver Cancer,” European Journal of Nutrition, Vol. 46, No. 5, 2007, pp. 293-299. http://dx.doi.org/10.1007/s00394-007-0665-8
[31]	D. Chang, F. Wang, Y. S. Zhao and H. Z. Pan, “Evaluation of Oxidative Stress in Colorectal Cancer Patients,” Biomedical and Environmental Sciences, Vol. 21, No. 4, 2008, pp. 286-289. http://dx.doi.org/10.1016/S0895-3988(08)60043-4
[32]	H. Kaynar, M. Meral, H. Turhan, M. Keles, G. Celik et al., “Glutathione Peroxidase, Glutathione-S-Transferase, Catalase, Xanthine Oxidase, Cu-Zn Superoxide Dismutase Activities, Total Glutathione, Nitric Oxide, and Malondialdehyde Levels in Erythrocytes of Patients with Small Cell and Non-Small Cell Lung Cancer,” Cancer Letters, Vol. 227, No. 2, 2005, pp. 133-139. http://dx.doi.org/10.1016/j.canlet.2004.12.005
[33]	C. C. Yeh, M. F. Hou, S. M. Tsai, S. K. Lin, J. K. Hsiao, J. C. Huang, et al., “Superoxide Anion Radical, Lipid Peroxides and Antioxidant Status in the Blood of Patients with Breast Cancer,” Clinica Chimica Acta, Vol. 361, No. 1-2, 2005, pp. 104-111. http://dx.doi.org/10.1016/j.cccn.2005.05.002
[34]	L. J. Niederhofer, J. S. Daniels, C. A. Rauzer, R. E. Greene and L. J. Marnett, “Malonaldehyde, a Product of Lipid Peroxidation ?s Mutagenic in Human Cells,” The Journal of Biological Chemistry, Vol. 278, No. 3, 2003, pp. 31426-31433. http://dx.doi.org/10.1074/jbc.M212549200
[35]	L. J. Marnett, “Oxy Radicals, Lipid Peroxidation and DNA Damage,” Toxicology, No. 181/182, 2002, pp. 219-222. http://dx.doi.org/10.1016/S0300-483X(02)00448-1
[36]	S. P. Hussain, L. J. Hofseth and C. C. Harris, “Radical Causes of Cancer,” Nature Reviews Cancer, Vol. 3, No. 4, 2003, pp. 276-285. http://dx.doi.org/10.1038/nrc1046
[37]	C. Nicco, A. Laurent, C. Chereau, B. Weill and F. Batteux, “Differential Modulation of Normal and Tumor Cell Proliferation by Reactive Oxygen Species,” Biomedicine & Pharmacotherapy, Vol. 59, No. 4, 2005, pp. 169-174. http://dx.doi.org/10.1016/j.biopha.2005.03.009
[38]	M. F. Polat, S. Taysi, M. Gul, O. C?kman, I. Yilmaz, E. Bakan and F. Erdogan, “Oxidant/Antioxidant Status in Blood of Patients with Malignant Breast Tumor and Benign Breast Disease,” Cell Biochemistry and Function, Vol. 20, No. 4, 2002, pp. 327-331. http://dx.doi.org/10.1002/cbf.980
[39]	C. A. Lang, S. Naryshkin, D. L. Schneider, B. J. Mills and R. D. Lindeman, “Low Blood Glutathione Levels in Healthy Aging Adults,” Journal of Laboratory and Clinical Medicine, Vol. 120, No. 5, 1992, pp. 720-725.
[40]	L. Masotti, E. Casali, N. Gesmundo, G. Sartor, T. Galeotti, S. Borrello, M. V. Pitetti and G. Pagl?uca, “Lipid Peroxidation in Cancer Cells: Chemical and Physical Studies,” Annals of the New York Academy of Sciences, Vol. 551, 1988, pp. 47-57. http://dx.doi.org/10.1111/j.1749-6632.1988.tb22319.x
[41]	A. T. Diplock, “Antioxidant Nutrients and Disease Prevention: An Overview,” American Journal of Clinical Nutrition, Vol. 53, No. 1, 1991, pp. 189-193.
[42]	K. T. Lee, S. M. Tsai, S. N. Wang, S. K. Lin, S. H. Wu, S. C. Chuang, S. H. Wu, H. Ma and L. Y. Tsai, “Glutathione Status in the Blood and Tissues of Patients with Virus-Originated Hepatocellular Carcinoma,” Clinical Biochemistry, Vol. 40, No. 15, 2007, pp. 1157-1162. http://dx.doi.org/10.1016/j.clinbiochem.2007.06.012
[43]	S. Kikkawa, M. Kadoharaa and H. Kawasaki, “Superoxide Dismutase and Glutathione in the Gastric Mucosa of Patients with Chronic Liver Disease,” Research Communications in Chemical Pathology and Pharmacology, Vol. 78, No. 3, 1992, pp. 289-309.
[44]	S. S. Chiou, T. T. Chang, S. P. Tsai, R. C. Jang, S. K. Lin, S. C. Lee, S. M. Tsai and L. Y. Tsai, “Lipid Peroxidation and Antioxidative Status in β-Thalassemia Major Patients with or without Hepatitis C Virus Infection,” Clinical Chemistry and Laboratory Medicine, Vol. 44, No. 10, 2006, pp. 1226-1233. http://dx.doi.org/10.1515/CCLM.2006.219
[45]	S. Abel, M. DeKock, D. J. Vanschalkwyk, S. Swanevelder, M. C. Kew and W. C. A. Gelderblom, “Altered Lipid Profile, Oxidative Status and Hepatitis B Virus Interactions in Human Hepatocellular Carcinoma,” Prostaglandins, Leukotrienes and Essential Fatty Acids, Vol. 81, No. 5, 2009, pp. 391-399. http://dx.doi.org/10.1016/j.plefa.2009.08.003
[46]	D. Scibior, M. Skrzycki, M. Podsiad and H. Czeczot, “Glutathione Level and Glutathione-Dependent Enzyme Activities in Blood Serum of Patients with Gastrointestinal Tract Tumors,” Clinical Biochemistry, Vol. 41, No. 10-11, 2008, pp. 852-858. http://dx.doi.org/10.1016/j.clinbiochem.2008. 03.005
[47]	A. Gonenc, A. Hacisevki, S. Aslan, M. Torun and B. Simsek, “Increased Oxidative DNA Damage and Impaired Antioxidant Defense System in Patients with Gastrointestinal Cancer,” European Journal of Internal Medicine, Vol. 23, No. 4, 2012, pp. 350-354. http://dx.doi.org/10.1016/j.ejim.2012.02.001