Effect of collagen and collagen peptides from bluefin tuna abdominal skin on cancer cells

Sung-Hee Han, Yuki Uzawa, Tatsuya Moriyama, Yukio Kawamura

Department of Applied Biological Chemistry, Graduate School of Agriculture, Kinki University.

DOI: 10.4236/health.2011.33024                

Abstract

In the present study, we investigated the effect of collagen and collagen peptides from bluefin tuna abdominal skin on cancer cells. Collagens were extracted from bluefin tuna (Thunnus orientails) abdominal, mackerel, and carp skin. The calf and salmon collagen were used reagent grade as a standard samples. The main protein band pattern produced by SDS-PAGE of all collagen samples consisted of two chains and one chain. For collagen peptides samples, bluefin tuna ab-dominal skin collagen and salmon skin collagen were hydrolyzed by trypsin. Among samples, salmon, mackerel, carp collagen, and their collagen peptides did not significantly reduce relative cell growth. However, the bluefin tuna abdominal skin collagen dramatically reduced HepG2 and HeLa cell growth by over 50% relative in a concentration-dependent manner when added to cells seeded 96-well plates. This suggests the collagen adding time was mightily important for effect of the collagen.

Keywords

Bluefin Tuna Collagen; Type I Collagen; Hepg2 Cell; Hela Cell

Conflicts of Interest

The authors declare no conflicts of interest.

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