Relative Bile Acid Binding Potential of Extruded Lentil Snacks


Previously we have reported that extrusion significantly improved healthful potential of cereals. It was hypothesized that snacks produced by extrusion would be more healthful than their raw formulations. Bile acid binding has been reported to indicate cholesterol lowering and cancer risk reduction potential of food and fractions. Bile acid binding potential of five lentil snack raw formulations and their extruded snacks were evaluated. The raw formulations were 100% lentils (F01), 69% lentils (F02), 57% lentils + 12% supplement (F03), F03 with 125 μg/100g Chromium (F04), F03 with 536 μg/100g Chromium (F05), and their respective extruded (E) snacks E01, E02, E03, E04 and E05. The in vitro bile acid binding on an equal dry matter basis relative to cholestyramine, was F01 (0.5%), E01 (3.7%), F02 (0.6%), E02 (3.0%), F03 (1.6%), E03 (5.1%), F04 (2.0%), E04 (4.2%), F05 (0.8%) and E05 (3.6%). Replacing 12% lentils with high protein supplements (F02 vs. F03) resulted in significantly higher bile acid binding, suggesting that the supplement appears to have higher bile acid binding capacity than that of lentils. All the extruded lentil snacks had significantly higher bile acid binding compared with their raw formulations. Extruding with added chromium containing yeast resulted in significantly lower bile acid binding in a dose dependent manner. Most healthful lentil snacks were produced with the addition of high protein supplement without added chromium-containing yeast (E03). Data proved the hypothesis that lentil snacks produced by extrusion are significantly more healthful than their raw formulations.

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T. Kahlon, J. Berrios, M. Chiu and J. Pan, "Relative Bile Acid Binding Potential of Extruded Lentil Snacks," Food and Nutrition Sciences, Vol. 5 No. 4, 2014, pp. 361-365. doi: 10.4236/fns.2014.54043.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] C. D. Mathers, A. D. Lopez and C. J. L. Murray, “The Burden of Disease and Mortality by Condition: Data, Methods, and Results for 2001,” In: A. D. Lopez, C. D. Mathers, M. Ezzati, D. T. Jamison and C. J. L. Murray, Eds., Global Burden of Disease and Risk Factors, Oxford University Press, New York.
[2] K. E. Suckling, G. M. Benson, B. Bond, A. Gee, A. Glen, C. Haynes, et al., “Cholesterol Lowering and Bile Acid Excretion in the Hamster with Cholestyramine Treatment,” Atherosclerosis, Vol. 89, No. 2, 1991, pp. 183-190.
[3] T. Nakamura and Y. Matsuzawa, “Drug Treatment of Hyperlipoproteinemia: Bile Acid-Binding Resins,” Nippon Rinsho, Vol. 52, No. 12, 1994, pp. 3266-3270.
[4] B. P. Daggy, N. C. O’Connell, G. R. Jerdack, B. A. Stinson and K. D. Setchell, “Additive Hypocholesterolemic Effect of Psyllium and Cholestyramine in the Hamster: Influence on Fecal Sterol and Bile Acid Profiles,” Journal of Lipid Research, Vol. 38, No. 3, 1997, pp. 491-502.
[5] T. S. Kahlon and F. I. Chow, “In Vitro Binding of Bile Acids by Rice Bran, Oat Bran, Wheat Bran and Corn Bran,” Cereal Chemistry, Vol. 77, No. 4, 2000, pp. 518521.
[6] A. F. Hofmann, “The Enterohepatic Circulation of Bile Acids in Man,” Clinics in Gastroenterology, Vol. 6, No. 1, 1977, pp. 3-24.
[7] J. Hageman, H. Herrema, A. K. Groen and F. Kuipers, “A Role of Bile Salt Receptor FXR in Atherosclerosis,” Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 30, 2010, pp. 1519-1528.
[8] H. C. Trowell, “Refined Foods and Disease,” Academic Press, London, 1975, pp. 195-226.
[9] E. K. Lund, J. M. Gee, J. C. Brown, P. J. Wood and I. T. Johnson, “Effect of Oat Gum on the Physical Properties of the Gastrointestinal Contents and on the Uptake of D-Galactose and Cholesterol by Rat Small Intestine in Vitro,” British Journal of Nutrition, Vol. 62, No. 1, 1989, pp. 91-101.
[10] J. W. Anderson and A. E. Siesel, “Hypocholesterolemic Effects of Oat Products,” In: I. Furda, C. J. Brine, Eds., New Developments in Dietary Fiber: Physiological, Physiochemical, and Analytical Aspects, Plenum Press, New York, 1990, pp. 17-36.
[11] V. Costarelli, T. J. Key, P. N. Appleby, D. S. Allen, I. S. Fentiman and T. A. Sanders, “A Prospective Study of Serum Bile Acid Concentrations and Colorectal Cancer Risk in Post-Menopausal Women on the Island of Guernsey,” British Journal of Cancer, Vol. 86, 2002, pp. 17411744.
[12] T. S. Kahlon and F. I. Chow, “In Vitro Binding of Bile Acids by Riceran, Oat Bran, Wheat Bran, and Corn Bran,” Cereal Chemistry, Vol. 77, No. 4, 2000, pp. 518-521.
[13] V. A. Meshcheriakova, O. A. Plotnikova, T. A. Iatsyshina, Kh. Kh. Sharafetdinov, M. L. Faivishevskii and T. N. Lisina, “New Extrusion Products in Diet Therapy of Diseases of Internal Organs,” Voprosy Pitaniia, Vol. 5, 1995, pp. 31-33.
[14] T. S. Kahlon, R. H. Edwards and F. I. Chow, “Effect of Extrusion on Hypocholesterolemic Properties of Rice, Oat, Corn, and Wheat Bran Diets in Hamsters,” Cereal Chemistry, Vol. 75, No. 6, 1998, pp. 897-903.
[15] T. S. Kahlon, J. De J. Berrios, G. E. Smith and J. L. Pan, “Evaluating Hypocholesterolemic Properties of Extruded Wheat Bran Diets in Hamsters,” Cereal Chemistry, Vol. 83, No. 2, 2006, pp. 152-156.
[16] T. S. Kahlon, J. De J. Berrios, G. E. Smith and J. L. Pan, “In Vitro Bile Acid Binding Capacity of Wheat Bran Extruded at Five Specific Mechanical Energy Levels,” Cereal Chemistry, Vol. 83, No. 2, 2006, pp. 157-160.
[17] D. Kritchevsky and J. A. Story, “Binding of Bile Salts in Vitro by Nonnutritive Fiber,” Journal of Nutrition, Vol. 104, No. 4, 1974, pp. 458-462.
[18] T. S. Kahlon and C. L. Woodruff, “In Vitro Binding of Bile Acids by Rice Bran, Oat Bran, Barley and ?-Glucan Enriched Barley,” Cereal Chemistry, Vol. 80, No. 3, 2003, pp. 260-263.
[19] T. S. Kahlon and C. L. Woodruff, “In Vitro Binding of Bile Acids by Various Ready to Eat Breakfast Cereals,” Cereal Foods World, Vol. 48, No. 2, 2003, pp. 73-75.
[20] M. C. Carey and D. M. Small, “The Characteristics of Mixed Micellar Solutions with Particular Reference to Bile,” American Journal of Medicine, Vol. 49, No. 5, 1970, pp. 590-608.
[21] S. S. Rossi, J. L. Converse and A. F. Hoffman, “High Pressure Liquid Chromatography Analysis of Conjugated Bile Acids in Human Bile: Simultaneous Resolution of Sulfated and Unsulfated Lithocholyl Amidates and the Common Conjugated Bile Acids,” Journal of Lipid Research, Vol. 28, No. 5, 1987, pp. 589-595.
[22] US Food and Drug Administration, “FDA Final Rule for Federal Labeling: Health Claims; Oats and Coronary Heart Disease,” Federal Register, Vol. 62, 1997, pp. 3584-3681.
[23] Joint Health Claims Initiative, “Final Report on Generic Health Claim for Oats and Reduction of Blood Cholesterol,” 2004.

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