Osmotic Stress Effect over Carbohydrate Production in a Native Starin of Scenedesmus sp.


The production of biofuels is currently presented as a possible answer in the search for sustainable alternatives for the total or partial substitution of fossil fuels. One of the most successful biofuels that have been developed is bioethanol. However, bioethanol production has been limited since it relies on the use of sugar cane or cereals. These materials are important sources of food and their demand as both a biofuel and a foodstuff has led to the price increase and may lead to possible shortages. Our group has focused on searching for native microalgae as sources of carbohydrates and bioethanol, with the goal of finding a sustainable source of bioethanol. Currently, twelve different strains which reach growth rates between 0.7 - 1.8 g/L and present carbohydrate production under osmotic shock conditions have been isolated. In this work, we demonstrate the results obtained with the Chlorella sp. [1] strain and the results obtained with the Scenedesmus sp. strain. The Scenedesmus sp. strain showed an increase in the production from 22 to 650 mg/sugar/g of biomass (dry weight), after 24 hours of osmotic shock with 0.1 M NaCl. The osmolytes which were produced after osmotic shock were identified as sucrose and trehalose, both of which are fermentable. These results demonstrate that this strain, through the photosynthetic pathway and osmotic shock, is a potential source of fermentable sugars.

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P. Bremauntz, L. Fernández-Linares and R. Cañizares-Villanueva, "Osmotic Stress Effect over Carbohydrate Production in a Native Starin of Scenedesmus sp.," Natural Resources, Vol. 5 No. 1, 2014, pp. 5-9. doi: 10.4236/nr.2014.51002.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] M. P. Bremauntz, L. G. Torres, L. O. Canizares, E. Duran, L. Fernándz, “Trehalose and Sucrose Osmolytes Accumulated by Algae as Potential Raw Material for Bioethanol,” Natural Resources, Vol. 2, No. 3, 2011, pp. 173-179.
[2] R. Joset and M. Jeanjean, “Dynamics of the Response of Cyanobacteria to Salt-Stress: Deciphering the Molecular Events,” Physiologia Plantarum, Vol. 96, No. 4, 1996, pp. 738-744. http://dx.doi.org/10.1111/j.1399-3054.1996.tb00251.x
[3] K. Satoh and K. Murata, “Stress Responses of Photosynthetic Organisms” Elsevier Science, Amsterdam, 1998.
[4] J. Chow, “Energy Resources and Global Development,” Science, Vol. 302, No. 5650, 2003, pp. 1528-1531.
[5] E. Blumwald, J. Mehlhorn and L. Packer, “Studies of Osmoregulation in Salt Adaptation of Cyanobacteria with ESR Spin-Probe Techniques,” Proceedings of the National Academy of Sciences, Vol. 80, No. 9, 1983, pp. 2599-2602. http://dx.doi.org/10.1073/pnas.80.9.2599
[6] D. Los and N. Murata, “Membrane Fluidity and Its Role in the Perseption of Environmental Signals,” Biochimica et Biophysica Acta, Vol. 1666, No. 1-2, 2004, pp. 142-157. http://dx.doi.org/10.1016/j.bbamem.2004.08.002
[7] A. Ben-Amotz and M. Avron, “Accumulation of Metabolites by Halotolerant Algae and Its Industrial Potential,” Annual Review of Microbiology, Vol. 37, 1983, pp. 95119.
[8] A. Oren, “Diversity of Organic Osmotic Compounds and Osmotic Adaptation in Cyanobacteria and Algae,” In: J. Seckbach, Ed., Algae and Cyanobacteria in Extreme Environments, Springer, Berlin, 2007, pp. 639-655.
[9] Goldember, “Ethanol for a Sustainable Energy Future,” Science, Vol. 315, No. 5813, 2007, pp. 808-810.
[10] G. Antolin, F. Tinaut, Y. Brinceno, V. Castano, C. Perez and A. Ramirez, “Optimisation of Diesel Production by Sunflower Oil Transesterification,” Bioresource Technology, Vol. 83, No. 2, 2002, pp. 111-114.
[11] J. R. Miranda, P. C. Passarinho and L. Gouveia, “PreTreatment Optimization of Scenedesmus obliquus Microalga for Bioethanol Production,” Bioresource Technology, Vol. 104, 2012, pp. 342-348.
[12] R. Rippka, D. Josette, J. Waterbury, M. Herdman and R. Y. Stanier, “Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria,” Journal of General Microbiology, Vol. 111, No. 1, 1979, pp. 1-61.
[13] M. Dubois, K. A. Gilles, J. K. Hamilton, P. A. Rebers and F. Smith, “Colorimetric Method for the Determination of Sugars and Related Substances,” Analytical Biochemistry, Vol. 28, No. 3, 1956, pp. 350-356.
[14] G. Hodaifa, M. Martínez, R. Orpez and S. Sanchez, “Inhibitory Effects of Industrial Olive-Oil Waste Water on Biomass Production of Scedesmus obliquus,” Ecological Engineering, Vol. 42, 2012, pp. 30-34.
[15] H. Shih-Hsin, C. Chun-Yen and C. Jo-Shu, “Effect of Light Intensity and Nitrogen Starvation on CO2 Fixation and Lipid/Carbohydrat Production of an Indigenous Microalga Scenedesmus obliquus CNW-N,” Bioresource Technology, Vol. 113, 2012, pp. 244-252.
[16] Y.-H. Wu, Y. Yu, X. Li, H.-Y. Hu and Z.-F. Su, “Biomass Production of a Scedesmus sp. under Phosphorous Starvation Cultivation Condition,” Bioresource Technology, Vol. 112, 2012, pp. 193-198.
[17] P. Siver and I. Trainor, “Morpholical Control and Physiology of Scenedesmus Strain 170,” Phycologia, Vol. 20, No. 1, 1981, pp. 1-11.
[18] I. Fedina and K. Benderliev, “Response of Scenedesmus incrasstulus to Salt Stress as Affected by Methyl Jamonate,” Biologia Plantarum, Vol. 43, No. 4, 2000, pp. 625-627. http://dx.doi.org/10.1023/A:1002816502941
[19] G. Demetriou, C. Neonaki, E. Navakoudis and K. Kotzabasis, “Salt Stress Impact on the Molecular Structure and Function of the Photosynthetic Apparatus—The Protective Role of Polyamines,” Biochimica et Biophysica Acta, Vol. 1767, No. 4, 2007, pp. 272-280.
[20] S. Shanab and H. Galal, “The Interactive Effect of Salinity and Urea on Growth, Some Related Metobolites an Antioxidants Enzymes of Chlorella sp. and Scenedesmus sp.,” New Egyptian Journal of Microbiology, Vol. 7, No. 2, 2007, pp. 64-75.
[21] A. B. El-Sayed, M. M El-Fouly and E. A. A. Abou ElNour, “Immobilized Microalga Scenedesmus sp. for Biological Desalination of Red Sea Water: I. Effect on Growth,” Nature and Science, Vol. 8, No. 9, 2010, pp. 69-76.
[22] S. Shu, L.-Y. Yuan, S.-R. Guo, J. Sun and C.-J. Liu, “Effects of Exogenous Spermidine on Photosynthesis, Xanthophyll Cycle and Endogenous Polyamines in Cucumber Seedlings Exposed to Salinity,” African Journal of Biotechnology, Vol. 11, No. 22, 2012, pp. 6064-6074.
[23] A. Kirrolia, N. Bishnoi and N. Singh, “Salinity as a Factor Affecting the Physiiologucal and Biochemical Trait of Scenedesmus quadricauda,” Journal of Algal Biomass Utilization, Vol. 2, No. 4, 2011, pp. 28-34.
[24] M. Page-Sharp; C. Behm and G. Smith, “Involvement of Compatible Solutes Trehalose and Sucrose in the Response to SALT Stress of Cyanobaterial Scytonema Species Isolated from Desert Solils,” Biochimica et Biophysica Acta, Vol. 1472, No. 3, 1999, pp. 519-528.
[25] G. Markou, I. Angelidaki and G. Dimitris, “Microbial Carbohydrates: An Overview of the Factors Influencing Carbohydrates Production, and of Main Bioconversion Technologies for Production of Biofuels,” Applied Microbiology and Biotechnology, Vol. 96, No. 3, 2012, pp. 631-645. http://dx.doi.org/10.1007/s00253-012-4398-0
[26] H. Gou, D. Maurycy, L. Liu, G. Qiu, S. Geng and G. Wang, “Biochemical Features and Bioethanol Production of Microalgae from Coastal Waters of Pearl River Delta,” Bioresource Technology, Vol. 127, 2013, pp. 422-428.
[27] P. J. Rojan, G. Anisha, K. Nampoothiri and A. Pandey, “Micro and Macroalgal Biomass: A Renewable Source for Bioethanol,” Bioresource Technology, Vol. 102, No. 1, 2011, pp. 186-193.

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