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Effects of Ascorbic Acid in Controlling Lethal Browning in in Vitro Culture of Brahylaena huillensis Using Nodal Segments

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DOI: 10.4236/ajps.2014.51024    5,078 Downloads   6,924 Views   Citations

ABSTRACT

Brachylaena huillensis (Asteraceae) is a threatened resourceful timber tree species. B. huillensis regenerates only through seeds. However, the seeds have poor germination rate and are also not obtainable. Developing tissue culture techniques for B. huillensis will permit the application of biotechnology to its propagation and provide alternative method for its regeneration. The current study was conducted to investigate the effect of antioxidant ascorbic acid in controlling lethal browning caused by oxidized phenols in in vitro culture of Brachylaena huillensis using nodal segments. The treatments included four levels of ascorbic acid (0, 50, 100, 150, 200, & 250 mg/litre) supplied into basal woody plant medium supplemented with Benzylaminopurine (BAP). The results of the current study revealed that production of phenolic compounds of explants was significantly controlled by incorporating higher levels of ascorbic acid into the medium. The best control was achieved by supplying 200-250 mg/litre of ascorbic acid in the woody plant medium supplemented with BAP.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

C. Ndakidemi, E. Mneney and P. Ndakidemi, "Effects of Ascorbic Acid in Controlling Lethal Browning in in Vitro Culture of Brahylaena huillensis Using Nodal Segments," American Journal of Plant Sciences, Vol. 5 No. 1, 2014, pp. 187-191. doi: 10.4236/ajps.2014.51024.

References

[1] S. K. Chonge, “Study of Economic Aspects of the Wood Carving Industry in Kenya: Implications for Policy Development to Make the Industry More Sustainable,” Thesis for Award of MSc Degree at University of Natal, Natal, South Africa, 2002, 55 p.
[2] WCMC, “Brachylaena Huillensis: IUCN Red List of Threatened Species,” 2008. http://www.iucnredlist.org
[3] C. K. Ruffo and S. M. Maliondo, “Forest Plant Genetic Resources in Tanzania,” In: F. M. Shao, F. S. Magingo, A. N. Minja, H. F. Bitanyi and R. L. Mahuna, Eds., Plant Genetic Resources and Biotechnology, Proceedings of the First National Workshop, Arusha, 1990, pp. 16-20.
[4] IUCN, “IUCN Red List of Threatened Species,” 2008.
http://www.iucnredlist.org
[5] L. P. Mbuya, H. P. Msanga, C. K. Ruffo, A. Birniel and B. Tengnas, “Useful Trees and Shrubs for Tanzania: Identification, Propagation and Management for Agricultural and Pastoral Communities,” Region Soil Conservation Unit, SIDA, Arusha, 1994, 542 p.
[6] A. B. Cunningham, “Kenya’s Carvings, the Ecological Footprint of the Wooden Rhino,” Africa Wildlife and Environment, Vol. 6, No. 2, 1998, pp. 43-50.
[7] J. M. Bryce and A. W. Chihongo, “The Commercial Timbers of Tanzania,” KAD Publishers, Dar es Salaam, 1999, 293 p.
[8] N. T. Marshall and M. Jenkins, “Hard Times for Hardwood: Indigenous Timber and the Timber Trade in Kenya,” Traffic International, London, 1994. 53 p.
[9] G. Laxmisita and B. V. Raghavaswamy, “Application of Biotechnology in Forest Trees Clonal Multiplication of Sandal Wood, Rose Wood, Teak, Eucalypts and Bamboos by Tissue Culture in India,” In: Puri, Ed., Tree Improvement, Oxford, 1998, pp. 233-248.
[10] M. R. Ahuja, “Microprapagation of Woody Plants,” Kluwer Academic Publication, Dordrechet, 1993, 507 p.
[11] T. A. Thorpe, I. S. Harry and P. P. Kumar, “Application of Micropropagation to Forestry,” In: P. C. Debergh and R. H. Zimmermann, Eds., Micropropagation Technology and Application, Kluwer Academic Publishers, Dordrecht, 1991, pp. 311-336.
[12] S. Seeni and P. G. Latha. “In Vitro Multiplication and Eco-Rehabilitation of the Endangered Blue Vanda,” Plant Cell, Tissue and Organ Culture, Vol. 61, 2000, pp. 1-8.
http://dx.doi.org/10.1023/A:1006444614657
[13] I. I. Ozyigit, “Phenolic Changes during in Vitro Organogenesis of Cotton (Gossypium hirsutum L) Shoot Tips,” African Journal of Biotechnology, Vol. 7, 2008, pp. 1145-1150.
[14] V. I. Kefeli, M. V. Kalevitch and B. Borsari, “Phenolic Cycle in Plants and North et al. 645 Environment,” Journal of Molecular Cell Biology, Vol. 2, 2003, pp. 13-18.
[15] M. Antolovich, P. Prenzler, K. Robards and D. Ryan, “Sample Preparation of Phenolic Compounds in Fruits,” Analyst, Vol. 125, 2000, pp. 989-1009.
http://dx.doi.org/10.1039/b000080i
[16] K. Robards, P. D. Prenzeler, G. Turcker, P. Swatsitang and. W. Glover, “Phenolic Compounds and Their Role in Oxidative Process in Fruits,” Food Chemistry, Vol. 66, 1999, pp. 401-436.
http://dx.doi.org/10.1016/S0308-8146(99)00093-X
[17] A. Lux-Endrich, D. Treautter and W. Feucht, “Influence of Nutrients and Carbohydrate Supply on the Phenol Composition of Apple Shoot Cultures,” Plant Cell, Tissue and Organ Culture, Vol. 60, 2000, pp. 15-21.
http://dx.doi.org/10.1023/A:1006406527242
[18] H. R. Kerns and M. M. Meyer Jr., “Tissue Culture Propagation of Acer Freemanii Using Thidiazuron to Stimulate Shoot Tip Proliferation,” HortScience, Vol. 21, 1986, pp. 1209-1210.
[19] H. Strosse, I. Van den Houwe and B. Panis, “Banana Cell and Tissue Culture—A Review,” In: S. M. Jain and R. Swennen, Eds., Banana Improvement: Cellular, Molecular Biology, and Induced Mutations, Science Publishers, Enfield, 2004.
[20] R. N. Abdelwahd, M. Hakam, S. M. Labhilili and Udupa, “Use of an Adsorbent and Antioxidants to Reduce the Effects of Leached Phenolics in in Vitro Plantlet Regeneration of Faba Bean,” African Journal of Biotechnology, Vol. 7, No. 8, 2008, pp. 997-1002.
[21] E. F. George, “Plant Propagation by Tissue Culture,” Parts 1 and 2, Edington, Wilts, Exegetics Ltd., Eversley, 1996.
[22] J. Arditti and R. Ernst, “Micropropagation of Orchids,” John Wiley and Sons, New York, 1993, p. 640.
[23] S. Titov, S. K. Bhowmik, A. Mandal, M. D. S. Alam and S. N. Uddin, “Control of Phenolic Compound Secretion and Effect of Growth Regulators for Organ Formation from Musa spp. cv. Kanthali Floral Bud Explants,” American Journal of Biochemistry and Biotechnology, Vol. 2, No. 3, 2006, pp. 97-104.
http://dx.doi.org/10.3844/ajbbsp.2006.97.104
[24] Y. He, X. Guo, R. Lu, B. Niu, V. Pasapula and P. Hou, “Changes in Morphology and Biochemical Indices in Browning Callus Derived from Jatropha curcas Hypocotyls,” Plant Cell, Tissue and Organ Culture, Vol. 98, 2009, pp. 11-17.
http://dx.doi.org/10.1007/s11240-009-9533-y
[25] M. Sujatha and N. Mukta, “Morphogenesis and Plant Regeneration from Tissue Cultures of Jatropha curcas,” Plant Cell, Tissue and Organ Culture, Vol. 44, 1996, pp. 135-141. http://dx.doi.org/10.1007/BF00048191
[26] Q. Wei, W. D. Lu, Y. Liao, S.-L. Pan, Y. Xu and L. Tang, “Plant Regeneration from Epicotyl Explant of Jatropha curcas,” Journal of Plant Physiology and Molecular Biology, Vol. 30, 2004, pp. 475-478.
[27] J. C. Lovett and T. Pocs, “Assessment of the Condition of the Catchment Forest Reserves: A Botanical Appraisal,” Government Printers, Dar es Salaam, 1993, 300 p.
[28] G. Lloyd and B. McCown, “Commercially Feasible Micropropagaation of Mountain Laurel, Kalmia latifolia, by Use of Shoot-Tip Culture,” Combined Proceedings, International Plant Propagators’ Society, Vol. 30, 1981, pp. 421-427.
[29] M. Ziv and A. H. Halevy, “Control of Oxidative Browning and in Vitro Propagation of Strelitzia reginae,” Hortscience, Vol. 18, No. 4, 1983, pp. 434-436.
[30] R. G. D. Steel and J. H. Torrie, “Principles and Procedures of Statistics,” 2nd Edition, McGraw Hill Book Co. Inc., New York, 1980, pp. 232-249.
[31] K. Welsh, K. C. Sink and H. Davidson, “Progress on in Vitro Propagation of Red Maple,” Combined Proceedings —International Plant Propagators’ Society, Vol. 29, 1979, pp. 382-386.
[32] P. Das, S. G. R. Samantaray and Rout, “In Vitro Propagation of Acacia Catechu, a Xerophilous Tree,” Plant Tissue Culture, Vol. 6, No. 2, 1996, pp. 117-126.
[33] W. H. Ko, C. L. Chen and C. P. Chao, “Control of Lethal Browning of Tissue Culture Plantlets of Cavendish Banana cv. Formosana with Ascorbic Acid,” Plant Cell, Tissue and Organ Culture, Vol. 96, 2009, pp. 137-147.
http://dx.doi.org/10.1007/s11240-008-9469-7
[34] P. A. Roussos and C. A. Pontikis, “Phenolic Compounds in Olive Explants and Their Contribution to Browning during the Establishment Stage in Vitro,” Gartenbauwissenschaft, Vol. 66, No. 6, 2001, pp. 298-303.
[35] T. L. Arnaldos, R. Munoz, M. A. Ferrer and A. A. Calderon, “Changes in Phenol Content during Strawberry (Fragaria x ananasa, cv. Chandler) Callus Culture,” Physiologia Plantarum, Vol. 113, No. 3, 2001, pp. 315-322.
http://dx.doi.org/10.1034/j.1399-3054.2001.1130303.x
[36] M. E. Compton and J. E. Preece, “Exudation and Explants Establishment,” NS International Association of Plant Tissue Culture, Vol. 50, 1986, pp. 9-18.
[37] H. Laukkanen, H. Häggman, S. Kontunen-Soppela and A. Hohtola, “Tissue Browning of in Vitro Cultures of Scots Pine: Role of Peroxidase and Polyphenol Oxidase,” Physiologia Plantarum, Vol. 106, 1999, pp. 337-343.
http://dx.doi.org/10.1034/j.1399-3054.1999.106312.x

  
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