Development and Validation of an HPLC Method for Simultaneous Determination of Nine Active Components in ‘Da-Chai-Hu-Tang’

DOI: 10.4236/cm.2011.21004   PDF   HTML     5,044 Downloads   10,054 Views   Citations


In this study, a simple, reliable and accurate method for the simultaneous separation and determination of naringin, hesperidin, neohesperidin, baicalin, wogonoside, baicalein, wogonin, emodin and chrysophanol in ‘Da-Chai-Hu-Tang’ was developed by reverse-phase high-performance liquid chromatography (RP-HPLC). The chromatographic separation was performed on an Agilent ZORBAX C18 column (250 mm × 4.6 mm i.d., 5.0 μm), and the mobile phase composed of methanol and water containing 1% (v/v) acetic acid was used to elute the targets in a gradient elution mode. The flow rate and detection wavelength were set at 0.8 ml/min and 280 nm, respectively. All calibration curves of the nine components expressed good linearities (r2≥0.9992) within the tested ranges. The RSD values demonstrated the intra- and inter-day precisions were less than 2.89%, and the recoveries of the investigated compounds were between 96.22% and 105.28%. The proposed method is simple, precise, specific, sensitive, and successfully applied to determine the nine marker compounds in ‘Da-Chai-Hu-Tang’ for quality control.

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L. Zheng and D. Dong, "Development and Validation of an HPLC Method for Simultaneous Determination of Nine Active Components in ‘Da-Chai-Hu-Tang’," Chinese Medicine, Vol. 2 No. 1, 2011, pp. 20-28. doi: 10.4236/cm.2011.21004.

Conflicts of Interest

The authors declare no conflicts of interest.


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