Efficient Protein Refolding Using Surfactants at High Final Protein Concentration

Hidetaka Noritomi; Yoshiyuki Kato; Satoru Kato

doi:10.4236/jsemat.2014.41002

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Journal of Surface Engineered Materials ... > Vol.4 No.1, January 2014

Efficient Protein Refolding Using Surfactants at High Final Protein Concentration ()

Hidetaka Noritomi, Yoshiyuki Kato, Satoru Kato

Department of Applied Chemistry, Tokyo Metropolitan University, Tokyo, Japan.

DOI: 10.4236/jsemat.2014.41002 PDF HTML 4,613 Downloads 6,377 Views Citations

Abstract

The refolding of denatured hen egg white lysozyme (HEWL) was examined by surfactants at a high final refolded HEWL concentration (1 mg/mL). Hexadecyltrimethylammonium bromide (CTAB) and sucrose fatty acid monoester (DK-SS) were used to dissolve denatured HEWL without denaturants such as guanidine hydrochloride (GuHCl) and urea. When denatured HEWL was perfectly dissolved in buffer solutions containing surfactants and dithiothreitol (DTT), the concentration of CTAB was about one-twentieth times less than that of DK-SS. The concentration of CTAB strongly affected the refolding yield, and the maximum refolding yield was obtained at 0.88 mM CTAB, which is around the critical micelle concentration of CTAB. The refolding yield was influenced by the molar ratio of oxidized glutathione (GSSG) to DTT, and the maximum refolding yield was obtained when [GSSG]/[DTT] was 1.5. The refolding yield was markedly dependent upon the solution pH of HEWL, and exhibited 80% at pH 5.2.

Keywords

Protein Refolding; Dilution Method; Surfactant; Hen Egg-White Lysozyme

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Noritomi, H. , Kato, Y. and Kato, S. (2014) Efficient Protein Refolding Using Surfactants at High Final Protein Concentration. Journal of Surface Engineered Materials and Advanced Technology, 4, 9-13. doi: 10.4236/jsemat.2014.41002.

Conflicts of Interest

The authors declare no conflicts of interest.

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