Qualitative Characterization and Differentiation of Digestates from Different Biowastes Using FTIR and Fluorescence Spectroscopies

DOI: 10.4236/jep.2011.21009   PDF   HTML     5,557 Downloads   10,290 Views   Citations


Anaerobic digestion of biomasses originates different products, the most abundant of which are methane and carbon dioxide. During this process, a 60-70% organic matter reduction occurs and the final product, the digestate, is charac- terized by high biological stability and high contents of recalcitrant organic molecules and nutrients. In the present work digestates obtained by different mixture of biomasses in a full-scale co-digestion plant operating in Italy were characterized as whole samples without any pre-treatment or extraction by means of Fourier transform infrared spec- troscopy and fluorescence spectroscopy in the synchronous-scan mode and results were compared to those obtained on the single fresh substrates. Biomasses considered were: beef cattle slurry, maize or sorghum silage, agro-industrial residues, olive residues and olive mill wastewater. These substrates exhibited typical spectra related to their different chemical composition. Results obtained on digestates provided evidence of distinctive characteristic of the final product as a function of the different composition of the biomasses loaded into the digestion plant. We concluded that FTIR and fluorescence spectra of digestates produced in a real co-digestion plant “inherit” the main spectroscopic features of the organic wastes from which they are produced. Spectroscopic techniques used in this work succeeded in qualitatively characterizing and differentiating digestates obtained from biomasses of different chemical composition.

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M. Provenzano, G. Iannuzzi, C. Fabbri and N. Senesi, "Qualitative Characterization and Differentiation of Digestates from Different Biowastes Using FTIR and Fluorescence Spectroscopies," Journal of Environmental Protection, Vol. 2 No. 1, 2011, pp. 83-89. doi: 10.4236/jep.2011.21009.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] J. M. S. Mata-Alvarez and P. Llabres, “Anaerobic Diges- tion of Organic Wastes. An Overview of Research Achievements and Perspective,” Bioresource Technology, Vol. 74, No. 1, 2000, pp. 3-16. doi:10.1016/S0960-8524(00)00023-7
[2] J. J. Lay, Y. Y. Li, T. Noike, J. Endo and S. Ishimoto “Analysis of Environmental Factors Affecting Methane Production from High Solids Organic Wastes,” Water Science and Technology, Vol. 35, No. 6-7, 1997, pp. 493-500 doi:10.1016/S0273-1223(97)00560-X
[3] S. Filidei, C. Masciandaro and B. Ceccanti “Anaerobic Digestion of Olive Mill Effluents: Evaluation of Wastewater Organic Load and Phytotoxicity Reduction,” Water, Air and Soil Pollution, Vol. 145, No. 1-4, 2003, pp. 79-94. doi:10.1023/A:1023619927495
[4] M. Hamdi, “Toxicity and Biodegradability of Olive Mill Wastewaters in Batch Anaerobic Digestion,” Applied Biochemistry and Biotechnology, Vol. 37, No. 2, 1992, pp. 155-163. doi:10.1007/BF02921667
[5] M. D. Greca, P. Monaco, G. Pinto,A. Pollio, L. Previtera and F. Temussi, 2001. “Phytoxicity of Low-Molecular Weightphenols from Olive Mille Wastewaters,” Bulletin of Environmental Contamination and Toxycology, Vol. 67, 2001, pp. 352-359. doi:10.1007/s001280132
[6] R. Borja, J. Alba and C. J. Banks, “Impact of the Main Phenolic Compounds of Olive Mill (OMW) Wastewaters on the Kinetics of Acetoclastic Methanogenesis,” Process Biochemistry, Vol. 32, 1997, pp. 121-133. doi:10.1016/S0032-9592(96)00055-6
[7] N. Senesi, T. M. Miano, M. R. Provenzano and G. Brunetti, “Spectroscopic and Compositional Comparative Characterization of IHSS Reference and Standard Humic Acids of Various Origin,” The Science of the Total Environment, Vol. 81, No. 82, 1989, pp. 143-156. doi:10.1016/0048-9697(89)90120-4
[8] N. Senesi, T. M. Miano, M. R. Provenzano and G. Brunetti, “Characterization, Differentiation and Classification of Humic Substances by Fluorescence Spectroscopy,” Soil Science, Vol. 152, No. 4, 1991, pp. 259-271. doi:10.1097/00010694-199110000-00004
[9] N. Senesi, T. M. Miano and M. R. Provenzano, “Fluorescence Spectroscopy as a Mean to Distinguish Fulvic and Humic Acids from Dissolved and Sedimentary Aquatic and Terrestrial Sources,” Humic Substances in the “Aquatic and Terrestrial Environment” Lecture Notes in Earth Sciences, Vol. 33, Springer-Verlag, Berlin, 1991, pp. 63-73.
[10] M. R. Provenzano, N. Senesi and G. Piccone, “Thermal and Spectroscopic Characterization of Organic Matter from Municipal Solid Wastes,” Compost Science & Utilization, Vol. 6, 1998, pp. 67-73.
[11] M. R. Provenzano, S. C. de Olivera, M. R. S. Silva and N. Senesi, “Assessment of Maturity Degree of Composts from Domestic Solid Wastes by Fluorescence and Fourier Infrared Spectroscopies,” Journal of Agricultural and Food Chemistry, Vol. 49, 2001, pp. 885-892. doi:10.1021/jf0106796
[12] X. Gomez, M. J. Cuetos, B. Tartarosky, M. F. Martinez-Nunez and A. Moran, “A Comparison of Analytical Techniques for Evaluating Food Waste Degradation by Anaerobic Digestion,” Bioprocess and Biosystem Engineering, Vol. 33, 2010, pp. 427-438 doi:10.1007/s00449-009-0343-8
[13] S. Piccinini, C. Fabbri and M. Soldano, “Monitoring and Assessment of Three Biogas Plants in Italy,” Proceeding of the International Conference “Biogas science”, Germany, 9-11 December 2009.
[14] APHA “Standard Methods for the Examination of Water and Wastewater,” 18th Edition, American Public Health Association, Washington, DC, USA, 1992.
[15] IRSA CNR “Metodi Analitici per le Acque,” Quaderni, Istituto Politecnico e Zecca dello Stato, Rome, Italy, 1994.
[16] T. M. Miano and N. Senesi, “Synchronous Excitation Fluorescence Spectroscopy Applied to Soil Humic Substances Chemistry,” The Science of the Total Environment, Vol. 117, No. 118, 1992, pp. 41-51. doi:10.1016/0048-9697(92)90071-Y
[17] X. Melamane, R. Tandlich and J. Burgess, “Anaerobic Digestion of Fungally Pre-Treated Wine Distillery Waste- water,” African Journal of Biotechnology, Vol. 6, No. 17, 2007, pp. 1990-1993
[18] W. Chen, P. Westerhoff, J. A. Leeheer and K. Booksh, “Fluorescence Excitation-Emission Matrix Regional Integration to Quantify Spectra for Dissolved Organic Matter,” Environmental Science & Technology, Vol. 37, 2003, pp. 5701-5710. doi:10.1021/es034354c

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